Details of the Target

General Information of Target

Target ID LDTP07458
Target Name RPA-related protein RADX (RADX)
Gene Name RADX
Gene ID 55086
Synonyms
CXorf57; RPA-related protein RADX; RPA-related and RAD51-antagonist, X-chromosome
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGESGQPEAGPSHAGLDWPNPERNRAGVPGGVIRRAGSQGPRSWIQKVLEQIMDSPRQC
VTPSEVVPVTVLAVQRYLLEDEPRDTVPKPPLYCYDVTISDGVYQEKCYLDPSLNSLVYQ
NILKVGIQMRISRVSCLYNEKRIGQGILCIDNVHCGETSDSISLETPFRNRAHQEKPERP
LRGGKSHYLALWNNEDPYGDIWLTDKQPEEHNFSDTKIISLSHLEMTWTNRRNFPALLVR
ILHKSKLRYYGKPDKKMIEPYQTFLEVADSSGTVSVIMWNALCPEWYKSLRVGLVLLLQD
YSVKKSYPFRIQPVPVDPQIKLISTMEICLNLRDPPTNIIIIPEKQVKPEWRLPKLNHRF
TTRSELDDMPENCICDVIGLLVFVGRVQRSKKKENREDFWSYRWIHIADGTSEQPFIVEL
FSTSQPEIFENIYPMAYFVCTQLKVVRNDNQVPKLLYLTTTNESGVFITGHRGQPYTYDA
KVKNFIQWIRTKSDSGEQKNMVIGGYYPYPPVPETFSKYSSSIKVESLLTAISEVRKEIE
DLQYREQKRIAIQGIITAIKYIPHSSATESASASETLRNANRPSTSQAARVEIQERNGKR
HQDDEPVNSQYFQTTSTNLSLSNKIRILQGPHANPVAVPQPGASVQTKGIKPGMPSIFNR
RANINANLQGKARKTISDRWESQLWREKKFGLIDHLHYSRVYPESIPRKFMFEHRKFLSD
QYNSQPAKYVPPEGRPPKLDDFKSARSLGHFEVTILGLNHEIAIDVAFLPMYCPEDIRTS
QIDTLLTSMNYSCAYPQDTTGNDRLPGPRAVAGDIIKAATELDRVHIVGILDICNLGNNK
VEVYLHKIYSPENTS
Target Bioclass
Other
Subcellular location
Chromosome
Function
Single-stranded DNA-binding protein recruited to replication forks to maintain genome stability. Prevents fork collapse by antagonizing the accumulation of RAD51 at forks to ensure the proper balance of fork remodeling and protection without interfering with the capacity of cells to complete homologous recombination of double-strand breaks.
Uniprot ID
Q6NSI4
Ensemble ID
ENST00000372544.6
HGNC ID
HGNC:25486

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO320 SNV: p.A571T DBIA    Probe Info 
HKGZCC SNV: p.F710L .
Ishikawa (Heraklio) 02 ER SNV: p.S412L .
KYSE150 SNV: p.F168Y .
LS180 SNV: p.K252N DBIA    Probe Info 
MELJUSO SNV: p.E266D DBIA    Probe Info 
NCIH2172 SNV: p.P641L .
NCIH23 SNV: p.I320F .
OCIAML2 SNV: p.I532T .
SNGM SNV: p.Y198C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C793(2.76); C108(2.75)  LDD0080  [1]
IA-alkyne
 Probe Info  		C149(0.00); C793(0.00)  LDD0165  [2]
IPM
 Probe Info  		C60(0.00); C283(0.00); C136(0.00); C108(0.00)  LDD2156  [3]
AOyne
 Probe Info  		13.40  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C136(0.91); C329(1.02)  LDD1507  [5]
 LDCM0215  AC10 HEK-293T C136(0.85); C329(1.00)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C136(1.02)  LDD1509  [5]
 LDCM0259  AC14 HEK-293T C136(1.07)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C136(1.00)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C136(0.95); C329(1.16)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C136(1.00); C329(0.74)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C136(0.92)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C136(0.92); C329(1.61)  LDD1518  [5]
 LDCM0282  AC22 HEK-293T C136(1.16)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C136(1.00)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C136(1.03)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C136(1.00); C329(1.11)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C136(0.90); C329(0.73)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C136(0.95)  LDD1526  [5]
 LDCM0290  AC3 HEK-293T C136(1.01)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C136(0.99)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C136(0.99)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C136(1.05)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C136(1.27); C329(1.28)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C136(0.97); C329(0.75)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C136(0.99)  LDD1535  [5]
 LDCM0299  AC38 HEK-293T C136(1.01)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C136(0.94)  LDD1539  [5]
 LDCM0302  AC40 HEK-293T C136(1.06)  LDD1541  [5]
 LDCM0303  AC41 HEK-293T C136(1.05); C329(1.25)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C136(0.85); C329(0.73)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C136(0.99)  LDD1544  [5]
 LDCM0308  AC46 HEK-293T C136(1.06)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C136(1.01)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C136(0.89)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C136(1.05); C329(1.16)  LDD1550  [5]
 LDCM0313  AC50 HEK-293T C136(0.89); C329(0.89)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C136(1.03)  LDD1553  [5]
 LDCM0317  AC54 HEK-293T C136(1.06)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C136(1.01)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C136(1.03)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C136(0.79); C329(1.03)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C136(0.92); C329(1.23)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C136(0.96)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C136(0.93)  LDD1562  [5]
 LDCM0326  AC62 HEK-293T C136(1.15)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C136(0.96)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C136(1.01)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C136(0.99)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C136(1.04)  LDD1569  [5]
 LDCM0356  AKOS034007680 HEK-293T C136(0.92); C329(1.01)  LDD1570  [5]
 LDCM0275  AKOS034007705 HEK-293T C136(1.12)  LDD1514  [5]
 LDCM0368  CL10 HEK-293T C136(1.19)  LDD1572  [5]
 LDCM0371  CL102 HEK-293T C136(1.17)  LDD1575  [5]
 LDCM0375  CL106 HEK-293T C136(0.96)  LDD1579  [5]
 LDCM0379  CL11 HEK-293T C136(1.13)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C136(0.84)  LDD1584  [5]
 LDCM0384  CL114 HEK-293T C136(0.90)  LDD1588  [5]
 LDCM0388  CL118 HEK-293T C136(1.16)  LDD1592  [5]
 LDCM0390  CL12 HEK-293T C136(1.20)  LDD1594  [5]
 LDCM0393  CL122 HEK-293T C136(1.08)  LDD1597  [5]
 LDCM0397  CL126 HEK-293T C136(1.01)  LDD1601  [5]
 LDCM0401  CL14 HEK-293T C136(1.11)  LDD1605  [5]
 LDCM0404  CL17 HEK-293T C136(0.94); C329(1.05)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C136(0.88); C329(1.01)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C136(1.08)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C136(1.00)  LDD1611  [5]
 LDCM0410  CL22 HEK-293T C136(1.00)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C136(1.10)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C136(1.25)  LDD1616  [5]
 LDCM0414  CL26 HEK-293T C136(0.88)  LDD1618  [5]
 LDCM0417  CL29 HEK-293T C136(0.78); C329(0.92)  LDD1621  [5]
 LDCM0419  CL30 HEK-293T C136(0.96); C329(1.20)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C136(1.11)  LDD1624  [5]
 LDCM0423  CL34 HEK-293T C136(1.03)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C136(1.11)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C136(1.23)  LDD1629  [5]
 LDCM0431  CL41 HEK-293T C136(0.75); C329(1.04)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C136(0.97); C329(0.99)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C136(1.01)  LDD1637  [5]
 LDCM0436  CL46 HEK-293T C136(1.02)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C136(1.11)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C136(0.89)  LDD1642  [5]
 LDCM0440  CL5 HEK-293T C136(0.97); C329(1.01)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C136(1.01)  LDD1645  [5]
 LDCM0444  CL53 HEK-293T C136(0.81); C329(0.80)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C136(0.90); C329(0.71)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C136(1.03)  LDD1649  [5]
 LDCM0449  CL58 HEK-293T C136(1.01)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C136(1.02)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C136(0.95); C329(0.63)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C136(0.93)  LDD1655  [5]
 LDCM0454  CL62 HEK-293T C136(1.02)  LDD1657  [5]
 LDCM0457  CL65 HEK-293T C136(1.04); C329(1.02)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C136(0.88); C329(1.01)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C136(0.96)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C136(1.12)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C136(1.33)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C136(0.98)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C136(1.02)  LDD1668  [5]
 LDCM0467  CL74 HEK-293T C136(0.73)  LDD1670  [5]
 LDCM0470  CL77 HEK-293T C136(1.17); C329(0.92)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C136(0.89); C329(0.73)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C136(0.98)  LDD1675  [5]
 LDCM0476  CL82 HEK-293T C136(1.03)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C136(1.00)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C136(1.07)  LDD1681  [5]
 LDCM0480  CL86 HEK-293T C136(1.14)  LDD1683  [5]
 LDCM0483  CL89 HEK-293T C136(1.00); C329(1.05)  LDD1686  [5]
 LDCM0485  CL90 HEK-293T C136(1.01); C329(0.75)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C136(1.03)  LDD1689  [5]
 LDCM0489  CL94 HEK-293T C136(1.12)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C136(1.06)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C136(1.06)  LDD1694  [5]
 LDCM0493  CL98 HEK-293T C136(0.91)  LDD1696  [5]
 LDCM0427  Fragment51 HEK-293T C136(1.00)  LDD1631  [5]
 LDCM0022  KB02 HCT 116 C793(2.76); C108(2.75)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C793(2.61); C108(4.65)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C793(3.60); C108(3.12)  LDD0082  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA mismatch repair protein Mlh1 (MLH1) DNA mismatch repair MutL/HexB family P40692
NudC domain-containing protein 3 (NUDCD3) . Q8IVD9

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402