General Information of Target

Target ID LDTP07432
Target Name Mitochondrial sodium/calcium exchanger protein (SLC8B1)
Gene Name SLC8B1
Gene ID 80024
Synonyms
NCKX6; NCLX; SLC24A6; Mitochondrial sodium/calcium exchanger protein; Na(+)/K(+)/Ca(2+)-exchange protein 6; Sodium/calcium exchanger protein, mitochondrial; Sodium/potassium/calcium exchanger 6; Solute carrier family 24 member 6; Solute carrier family 8 member B1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGRRLNLRWALSVLCVLLMAETVSGTRGSSTGAHISPQFPASGVNQTPVVDCRKVCGLN
VSDRCDFIRTNPDCHSDGGYLDYLEGIFCHFPPSLLPLAVTLYVSWLLYLFLILGVTAAK
FFCPNLSAISTTLKLSHNVAGVTFLAFGNGAPDIFSALVAFSDPHTAGLALGALFGAGVL
VTTVVAGGITILHPFMAASRPFFRDIVFYMVAVFLTFLMLFRGRVTLAWALGYLGLYVFY
VVTVILCTWIYQRQRRGSLFCPMPVTPEILSDSEEDRVSSNTNSYDYGDEYRPLFFYQET
TAQILVRALNPLDYMKWRRKSAYWKALKVFKLPVEFLLLLTVPVVDPDKDDQNWKRPLNC
LHLVISPLVVVLTLQSGTYGVYEIGGLVPVWVVVVIAGTALASVTFFATSDSQPPRLHWL
FAFLGFLTSALWINAAATEVVNILRSLGVVFRLSNTVLGLTLLAWGNSIGDAFSDFTLAR
QGYPRMAFSACFGGIIFNILVGVGLGCLLQISRSHTEVKLEPDGLLVWVLAGALGLSLVF
SLVSVPLQCFQLSRVYGFCLLLFYLNFLVVALLTEFGVIHLKSM
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, SLC24A subfamily
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, by mediating the exchange of 3 sodium ions per 1 calcium ion. Plays a central role in mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion: calcium efflux is essential for mitochondrial function and cell survival, notably in cardiomyocytes. Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses. Required for store-operated Ca(2+) entry (SOCE) and Ca(2+) release-activated Ca(2+) (CRAC) channel regulation: sodium transport by SLC8B1 leads to promote calcium-shuttling that modulates mitochondrial redox status, thereby regulating SOCE activity. Involved in B-lymphocyte chemotaxis. Able to transport Ca(2+) in exchange of either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange. In contrast to other members of the family its function is independent of K(+).
TTD ID
T32232
Uniprot ID
Q6J4K2
DrugMap ID
TTJN46R
Ensemble ID
ENST00000202831.7
HGNC ID
HGNC:26175
ChEMBL ID
CHEMBL3763001

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.N71S .
HT115 SNV: p.D205N; p.E275D .
MDST8 SNV: p.R307W .
MELJUSO SNV: p.C89F .
MFE319 SNV: p.L401F .
RL952 SNV: p.L509I .
RVH421 SNV: p.P389S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info 
C123(0.97)  LDD1509  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C123(0.97)  LDD1509  [1]
 LDCM0259  AC14 HEK-293T C123(0.97)  LDD1512  [1]
 LDCM0278  AC19 HEK-293T C123(0.91)  LDD1517  [1]
 LDCM0281  AC21 HEK-293T C123(1.09)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C123(1.02)  LDD1521  [1]
 LDCM0284  AC24 HEK-293T C123(0.81)  LDD1523  [1]
 LDCM0287  AC27 HEK-293T C123(0.89)  LDD1526  [1]
 LDCM0289  AC29 HEK-293T C123(1.06)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C123(0.77)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C123(0.93)  LDD1530  [1]
 LDCM0293  AC32 HEK-293T C123(0.86)  LDD1532  [1]
 LDCM0296  AC35 HEK-293T C123(0.92)  LDD1535  [1]
 LDCM0298  AC37 HEK-293T C123(0.83)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C123(0.83)  LDD1538  [1]
 LDCM0302  AC40 HEK-293T C123(1.05)  LDD1541  [1]
 LDCM0305  AC43 HEK-293T C123(0.86)  LDD1544  [1]
 LDCM0307  AC45 HEK-293T C123(1.07)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C123(1.32)  LDD1547  [1]
 LDCM0310  AC48 HEK-293T C123(0.98)  LDD1549  [1]
 LDCM0312  AC5 HEK-293T C123(0.92)  LDD1551  [1]
 LDCM0314  AC51 HEK-293T C123(0.95)  LDD1553  [1]
 LDCM0316  AC53 HEK-293T C123(1.08)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C123(1.12)  LDD1556  [1]
 LDCM0319  AC56 HEK-293T C123(1.03)  LDD1558  [1]
 LDCM0322  AC59 HEK-293T C123(0.90)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C123(1.11)  LDD1562  [1]
 LDCM0325  AC61 HEK-293T C123(0.99)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C123(1.10)  LDD1565  [1]
 LDCM0328  AC64 HEK-293T C123(0.99)  LDD1567  [1]
 LDCM0345  AC8 HEK-293T C123(1.09)  LDD1569  [1]
 LDCM0248  AKOS034007472 HEK-293T C123(0.85)  LDD1511  [1]
 LDCM0275  AKOS034007705 HEK-293T C123(0.95)  LDD1514  [1]
 LDCM0367  CL1 HEK-293T C123(1.17)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C123(3.85)  LDD1572  [1]
 LDCM0369  CL100 HEK-293T C123(0.99)  LDD1573  [1]
 LDCM0370  CL101 HEK-293T C123(1.03)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C123(1.18)  LDD1575  [1]
 LDCM0373  CL104 HEK-293T C123(1.04)  LDD1577  [1]
 LDCM0374  CL105 HEK-293T C123(1.05)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C123(0.93)  LDD1579  [1]
 LDCM0377  CL108 HEK-293T C123(0.87)  LDD1581  [1]
 LDCM0378  CL109 HEK-293T C123(1.33)  LDD1582  [1]
 LDCM0380  CL110 HEK-293T C123(1.06)  LDD1584  [1]
 LDCM0382  CL112 HEK-293T C123(0.83)  LDD1586  [1]
 LDCM0383  CL113 HEK-293T C123(1.68)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C123(0.97)  LDD1588  [1]
 LDCM0386  CL116 HEK-293T C123(0.78)  LDD1590  [1]
 LDCM0387  CL117 HEK-293T C123(0.92)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C123(0.89)  LDD1592  [1]
 LDCM0390  CL12 HEK-293T C123(0.97)  LDD1594  [1]
 LDCM0391  CL120 HEK-293T C123(0.77)  LDD1595  [1]
 LDCM0392  CL121 HEK-293T C123(1.18)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C123(1.08)  LDD1597  [1]
 LDCM0395  CL124 HEK-293T C123(1.06)  LDD1599  [1]
 LDCM0396  CL125 HEK-293T C123(1.32)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C123(0.93)  LDD1601  [1]
 LDCM0399  CL128 HEK-293T C123(1.13)  LDD1603  [1]
 LDCM0400  CL13 HEK-293T C123(1.01)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C123(0.76)  LDD1605  [1]
 LDCM0403  CL16 HEK-293T C123(0.83)  LDD1607  [1]
 LDCM0406  CL19 HEK-293T C123(0.98)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C123(0.93)  LDD1611  [1]
 LDCM0409  CL21 HEK-293T C123(1.35)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C123(1.28)  LDD1614  [1]
 LDCM0412  CL24 HEK-293T C123(0.99)  LDD1616  [1]
 LDCM0413  CL25 HEK-293T C123(1.32)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C123(0.89)  LDD1618  [1]
 LDCM0416  CL28 HEK-293T C123(0.61)  LDD1620  [1]
 LDCM0420  CL31 HEK-293T C123(1.00)  LDD1624  [1]
 LDCM0422  CL33 HEK-293T C123(5.33)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C123(1.16)  LDD1627  [1]
 LDCM0425  CL36 HEK-293T C123(0.96)  LDD1629  [1]
 LDCM0426  CL37 HEK-293T C123(1.22)  LDD1630  [1]
 LDCM0429  CL4 HEK-293T C123(1.24)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C123(0.88)  LDD1634  [1]
 LDCM0433  CL43 HEK-293T C123(0.98)  LDD1637  [1]
 LDCM0435  CL45 HEK-293T C123(1.18)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C123(1.20)  LDD1640  [1]
 LDCM0438  CL48 HEK-293T C123(0.98)  LDD1642  [1]
 LDCM0439  CL49 HEK-293T C123(0.97)  LDD1643  [1]
 LDCM0441  CL50 HEK-293T C123(1.02)  LDD1645  [1]
 LDCM0443  CL52 HEK-293T C123(0.99)  LDD1646  [1]
 LDCM0446  CL55 HEK-293T C123(0.71)  LDD1649  [1]
 LDCM0448  CL57 HEK-293T C123(1.88)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C123(0.98)  LDD1652  [1]
 LDCM0452  CL60 HEK-293T C123(1.12)  LDD1655  [1]
 LDCM0453  CL61 HEK-293T C123(1.03)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C123(0.74)  LDD1657  [1]
 LDCM0456  CL64 HEK-293T C123(0.93)  LDD1659  [1]
 LDCM0459  CL67 HEK-293T C123(0.77)  LDD1662  [1]
 LDCM0461  CL69 HEK-293T C123(1.04)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C123(1.12)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C123(1.08)  LDD1666  [1]
 LDCM0465  CL72 HEK-293T C123(1.13)  LDD1668  [1]
 LDCM0466  CL73 HEK-293T C123(1.05)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C123(0.81)  LDD1670  [1]
 LDCM0469  CL76 HEK-293T C123(0.73)  LDD1672  [1]
 LDCM0472  CL79 HEK-293T C123(0.86)  LDD1675  [1]
 LDCM0475  CL81 HEK-293T C123(0.93)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C123(1.27)  LDD1679  [1]
 LDCM0478  CL84 HEK-293T C123(1.68)  LDD1681  [1]
 LDCM0479  CL85 HEK-293T C123(0.84)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C123(0.98)  LDD1683  [1]
 LDCM0482  CL88 HEK-293T C123(0.93)  LDD1685  [1]
 LDCM0484  CL9 HEK-293T C123(1.15)  LDD1687  [1]
 LDCM0486  CL91 HEK-293T C123(0.75)  LDD1689  [1]
 LDCM0488  CL93 HEK-293T C123(1.32)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C123(1.41)  LDD1692  [1]
 LDCM0491  CL96 HEK-293T C123(1.04)  LDD1694  [1]
 LDCM0492  CL97 HEK-293T C123(1.30)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C123(0.80)  LDD1696  [1]
 LDCM0427  Fragment51 HEK-293T C123(0.85)  LDD1631  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402