Details of the Target

General Information of Target

Target ID LDTP07406
Target Name GRAM domain-containing protein 4 (GRAMD4)
Gene Name GRAMD4
Gene ID 23151
Synonyms
DIP; KIAA0767; GRAM domain-containing protein 4; Death-inducing protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTL
IMATGVQDFNRTEFDRLNEIKGHLEIALLEKHFLQEELRKLREETNAEMLRQELDRERQR
RMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQGLSSRLQKWFYERFGEYVED
FRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSALSDWYSVYTSAIAFTV
YMNAVWHGWAIPLFLFLAILRLSLNYLIARGWRIQWSIVPEVSEPVEPPKEDLTVSEKFQ
LVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVGLA
VGLYAGIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERSSAAVSRRLQTTSSR
SYVPSAPAGLGKEEDAGRFHSTKKGNFHEIFNLTENERPLAVCENGWRCCLINRDRKMPT
DYIRNGVLYVTENYLCFESSKSGSSKRNKVIKLVDITDIQKYKVLSVLPGSGMGIAVSTP
STQKPLVFGAMVHRDEAFETILSQYIKITSAAASGGDS
Target Bioclass
Other
Subcellular location
Mitochondrion membrane
Function
Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. Inhibits TLR9 response to nucelic acids and regulates TLR9-mediated innate immune response.
Uniprot ID
Q6IC98
Ensemble ID
ENST00000361034.7
HGNC ID
HGNC:29113

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC1937 SNV: p.F74I .
HCT116 SNV: p.R270M .
HCT15 SNV: p.Q310H .
Ishikawa (Heraklio) 02 ER SNV: p.R121W .
SKNSH SNV: p.S406Y .
SNU1 SNV: p.I568T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y177(8.38)  LDD0260  [1]
STPyne
 Probe Info  		K385(5.56)  LDD0277  [2]
DBIA
 Probe Info  		C463(0.84)  LDD1511  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C388
 Probe Info  		99.73  LDD2047  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C463(1.04)  LDD1512  [3]
 LDCM0281  AC21 HEK-293T C463(0.90)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C463(1.06)  LDD1521  [3]
 LDCM0284  AC24 HEK-293T C463(1.04)  LDD1523  [3]
 LDCM0289  AC29 HEK-293T C463(0.94)  LDD1528  [3]
 LDCM0291  AC30 HEK-293T C463(1.25)  LDD1530  [3]
 LDCM0293  AC32 HEK-293T C463(0.83)  LDD1532  [3]
 LDCM0298  AC37 HEK-293T C463(0.98)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C463(0.96)  LDD1538  [3]
 LDCM0302  AC40 HEK-293T C463(1.04)  LDD1541  [3]
 LDCM0307  AC45 HEK-293T C463(0.94)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C463(1.05)  LDD1547  [3]
 LDCM0310  AC48 HEK-293T C463(1.13)  LDD1549  [3]
 LDCM0312  AC5 HEK-293T C463(0.94)  LDD1551  [3]
 LDCM0316  AC53 HEK-293T C463(0.88)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C463(1.24)  LDD1556  [3]
 LDCM0319  AC56 HEK-293T C463(0.81)  LDD1558  [3]
 LDCM0323  AC6 HEK-293T C463(1.00)  LDD1562  [3]
 LDCM0325  AC61 HEK-293T C463(0.87)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C463(1.07)  LDD1565  [3]
 LDCM0328  AC64 HEK-293T C463(0.96)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C463(1.08)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C463(0.84)  LDD1511  [3]
 LDCM0275  AKOS034007705 HEK-293T C463(0.98)  LDD1514  [3]
 LDCM0368  CL10 HEK-293T C463(0.85)  LDD1572  [3]
 LDCM0390  CL12 HEK-293T C463(0.88)  LDD1594  [3]
 LDCM0409  CL21 HEK-293T C463(0.98)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C463(0.97)  LDD1614  [3]
 LDCM0412  CL24 HEK-293T C463(0.90)  LDD1616  [3]
 LDCM0422  CL33 HEK-293T C463(0.84)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C463(1.15)  LDD1627  [3]
 LDCM0425  CL36 HEK-293T C463(0.83)  LDD1629  [3]
 LDCM0435  CL45 HEK-293T C463(0.91)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C463(0.97)  LDD1640  [3]
 LDCM0438  CL48 HEK-293T C463(0.88)  LDD1642  [3]
 LDCM0448  CL57 HEK-293T C463(0.81)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C463(0.99)  LDD1652  [3]
 LDCM0452  CL60 HEK-293T C463(0.91)  LDD1655  [3]
 LDCM0461  CL69 HEK-293T C463(1.12)  LDD1664  [3]
 LDCM0463  CL70 HEK-293T C463(1.15)  LDD1666  [3]
 LDCM0465  CL72 HEK-293T C463(1.03)  LDD1668  [3]
 LDCM0475  CL81 HEK-293T C463(0.96)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C463(1.16)  LDD1679  [3]
 LDCM0478  CL84 HEK-293T C463(0.98)  LDD1681  [3]
 LDCM0484  CL9 HEK-293T C463(0.98)  LDD1687  [3]
 LDCM0488  CL93 HEK-293T C463(0.89)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C463(1.18)  LDD1692  [3]
 LDCM0491  CL96 HEK-293T C463(1.06)  LDD1694  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Myotubularin-related protein 9 (MTMR9) Protein-tyrosine phosphatase family Q96QG7
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Exocyst complex component 8 (EXOC8) EXO84 family Q8IYI6
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
Growth arrest and DNA damage-inducible protein GADD45 gamma (GADD45G) GADD45 family O95257
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 (SMARCD1) SMARCD family Q96GM5
Cation channel sperm-associated targeting subunit tau (C2CD6) . Q53TS8
Coiled-coil domain-containing protein 146 (CCDC146) . Q8IYE0
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4
SH2 domain-containing protein 4A (SH2D4A) . Q9H788

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587