Details of the Target

General Information of Target

Target ID LDTP07116
Target Name Protrudin (ZFYVE27)
Gene Name ZFYVE27
Gene ID 118813
Synonyms
SPG33; Protrudin; Spastic paraplegia 33 protein; Zinc finger FYVE domain-containing protein 27
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDG
VRYLLRWQMPLCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDS
ELMRRKYHSVRQEDLQRGRLSRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPV
VSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEE
HAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLE
DDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVL
KKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK
Target Bioclass
Transporter and channel
Subcellular location
Recycling endosome membrane
Function
Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport. Promotes axonal elongation and contributes to the establishment of neuronal cell polarity. Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections. Acts as an adapter protein and facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner.
Uniprot ID
Q5T4F4
Ensemble ID
ENST00000337540.11
HGNC ID
HGNC:26559

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A549 SNV: p.L78M .
DU145 SNV: p.L71F .
HEC1 SNV: p.C350R .
HEC1B SNV: p.C350R .
RKO SNV: p.R116Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C374(0.98); C402(1.27); C165(1.11)  LDD1507  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C374(0.98); C402(1.27); C165(1.11)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C374(1.18); C402(0.97)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C374(1.06); C165(0.95)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C165(1.08)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C165(1.11)  LDD1512  [1]
 LDCM0270  AC15 HEK-293T C374(1.10); C165(1.13)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C374(1.04); C402(0.93); C165(1.08)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C374(0.80); C402(0.99)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C374(1.06); C165(0.66)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C374(0.98); C402(0.94)  LDD1518  [1]
 LDCM0280  AC20 HEK-293T C165(1.01)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C165(0.93)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C165(1.06)  LDD1521  [1]
 LDCM0283  AC23 HEK-293T C374(1.19); C165(0.94)  LDD1522  [1]
 LDCM0285  AC25 HEK-293T C374(1.06); C402(1.15); C165(0.98)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C374(1.11); C402(0.95)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C374(1.09); C165(0.91)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C165(0.91)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C165(0.88)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C374(1.23); C165(0.85)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C165(0.93)  LDD1530  [1]
 LDCM0292  AC31 HEK-293T C374(0.99); C165(0.96)  LDD1531  [1]
 LDCM0294  AC33 HEK-293T C374(1.20); C402(1.05); C165(1.11)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C374(1.17); C402(1.14)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C374(1.07); C165(1.01)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C165(0.96)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C165(0.84)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C165(1.06)  LDD1538  [1]
 LDCM0300  AC39 HEK-293T C374(1.14); C165(1.04)  LDD1539  [1]
 LDCM0301  AC4 HEK-293T C165(0.92)  LDD1540  [1]
 LDCM0303  AC41 HEK-293T C374(1.16); C402(0.89); C165(1.16)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C374(1.14); C402(0.95)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C374(1.03); C165(0.84)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C165(1.01)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C165(1.01)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C165(0.98)  LDD1547  [1]
 LDCM0309  AC47 HEK-293T C374(1.18); C165(1.01)  LDD1548  [1]
 LDCM0311  AC49 HEK-293T C374(1.00); C402(1.01); C165(1.15)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C165(0.85)  LDD1551  [1]
 LDCM0313  AC50 HEK-293T C374(1.23); C402(0.89)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C374(1.14); C165(0.95)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C165(1.04)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C165(0.94)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C165(0.81)  LDD1556  [1]
 LDCM0318  AC55 HEK-293T C374(1.18); C165(1.03)  LDD1557  [1]
 LDCM0320  AC57 HEK-293T C374(1.03); C402(1.07); C165(1.10)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C374(0.88); C402(0.87)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C374(1.02); C165(0.75)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C165(1.07)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C165(1.00)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C165(0.98)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C165(1.14)  LDD1565  [1]
 LDCM0327  AC63 HEK-293T C374(1.01); C165(1.00)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C374(1.09); C165(1.01)  LDD1568  [1]
 LDCM0248  AKOS034007472 HEK-293T C165(0.93)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C374(1.04); C402(0.90); C165(1.02)  LDD1570  [1]
 LDCM0367  CL1 HEK-293T C374(0.84); C165(1.00)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C165(2.64)  LDD1572  [1]
 LDCM0369  CL100 HEK-293T C165(1.15)  LDD1573  [1]
 LDCM0370  CL101 HEK-293T C374(1.04); C165(1.09)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C165(1.05)  LDD1575  [1]
 LDCM0372  CL103 HEK-293T C165(1.11)  LDD1576  [1]
 LDCM0373  CL104 HEK-293T C165(1.01)  LDD1577  [1]
 LDCM0374  CL105 HEK-293T C374(1.03); C165(1.10)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C165(1.03)  LDD1579  [1]
 LDCM0376  CL107 HEK-293T C165(0.87)  LDD1580  [1]
 LDCM0377  CL108 HEK-293T C165(0.99)  LDD1581  [1]
 LDCM0378  CL109 HEK-293T C374(0.98); C165(1.03)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C374(0.92); C165(1.38)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C165(1.14)  LDD1584  [1]
 LDCM0381  CL111 HEK-293T C165(1.09)  LDD1585  [1]
 LDCM0382  CL112 HEK-293T C165(1.01)  LDD1586  [1]
 LDCM0383  CL113 HEK-293T C374(0.99); C165(1.12)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C165(1.31)  LDD1588  [1]
 LDCM0385  CL115 HEK-293T C165(1.11)  LDD1589  [1]
 LDCM0386  CL116 HEK-293T C165(1.01)  LDD1590  [1]
 LDCM0387  CL117 HEK-293T C374(0.98); C165(1.12)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C165(0.97)  LDD1592  [1]
 LDCM0389  CL119 HEK-293T C165(1.08)  LDD1593  [1]
 LDCM0391  CL120 HEK-293T C165(1.03)  LDD1595  [1]
 LDCM0392  CL121 HEK-293T C374(1.13); C165(1.16)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C165(1.04)  LDD1597  [1]
 LDCM0394  CL123 HEK-293T C165(1.38)  LDD1598  [1]
 LDCM0395  CL124 HEK-293T C165(1.10)  LDD1599  [1]
 LDCM0396  CL125 HEK-293T C374(0.97); C165(1.03)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C165(1.21)  LDD1601  [1]
 LDCM0398  CL127 HEK-293T C165(1.01)  LDD1602  [1]
 LDCM0399  CL128 HEK-293T C165(1.02)  LDD1603  [1]
 LDCM0400  CL13 HEK-293T C374(1.07); C165(1.18)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C165(1.10)  LDD1605  [1]
 LDCM0402  CL15 HEK-293T C165(1.44)  LDD1606  [1]
 LDCM0403  CL16 HEK-293T C165(1.10)  LDD1607  [1]
 LDCM0404  CL17 HEK-293T C374(1.13); C402(0.90); C165(1.54)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C374(1.23); C402(0.99)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C374(1.06); C165(0.88)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C165(1.19)  LDD1611  [1]
 LDCM0408  CL20 HEK-293T C165(1.31)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C165(1.23)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C165(1.15)  LDD1614  [1]
 LDCM0411  CL23 HEK-293T C374(0.96); C165(1.29)  LDD1615  [1]
 LDCM0413  CL25 HEK-293T C374(0.92); C165(1.14)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C165(0.93)  LDD1618  [1]
 LDCM0415  CL27 HEK-293T C165(0.83)  LDD1619  [1]
 LDCM0416  CL28 HEK-293T C165(1.13)  LDD1620  [1]
 LDCM0417  CL29 HEK-293T C374(1.02); C402(1.03); C165(1.05)  LDD1621  [1]
 LDCM0418  CL3 HEK-293T C165(1.06)  LDD1622  [1]
 LDCM0419  CL30 HEK-293T C374(1.11); C402(0.96)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C374(1.03); C165(0.84)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C165(1.33)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C165(2.10)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C165(1.61)  LDD1627  [1]
 LDCM0424  CL35 HEK-293T C374(0.95); C165(1.38)  LDD1628  [1]
 LDCM0426  CL37 HEK-293T C374(0.91); C165(1.09)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C165(1.27)  LDD1632  [1]
 LDCM0429  CL4 HEK-293T C165(1.04)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C165(1.05)  LDD1634  [1]
 LDCM0431  CL41 HEK-293T C374(0.94); C402(0.96); C165(1.24)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C374(0.82); C402(0.87)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C374(1.07); C165(0.84)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C165(1.41)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C165(1.18)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C165(1.49)  LDD1640  [1]
 LDCM0437  CL47 HEK-293T C374(1.14); C165(1.28)  LDD1641  [1]
 LDCM0439  CL49 HEK-293T C374(1.01); C165(1.09)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C374(1.12); C402(0.91); C165(1.29)  LDD1644  [1]
 LDCM0441  CL50 HEK-293T C165(1.19)  LDD1645  [1]
 LDCM0443  CL52 HEK-293T C165(1.04)  LDD1646  [1]
 LDCM0444  CL53 HEK-293T C374(1.01); C402(0.92); C165(1.17)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C374(1.18); C402(0.91)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C374(1.08); C165(0.83)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C165(1.31)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C165(1.22)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C165(1.10)  LDD1652  [1]
 LDCM0450  CL59 HEK-293T C374(1.04); C165(1.31)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C374(1.12); C402(0.93)  LDD1654  [1]
 LDCM0453  CL61 HEK-293T C374(0.96); C165(1.07)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C165(1.04)  LDD1657  [1]
 LDCM0455  CL63 HEK-293T C165(0.94)  LDD1658  [1]
 LDCM0456  CL64 HEK-293T C165(1.26)  LDD1659  [1]
 LDCM0457  CL65 HEK-293T C374(1.05); C402(0.95); C165(1.09)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C374(1.05); C402(1.14)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C374(1.10); C165(0.89)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C165(1.32)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C165(1.20)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C374(1.11); C165(0.86)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C165(1.61)  LDD1666  [1]
 LDCM0464  CL71 HEK-293T C374(0.96); C165(1.45)  LDD1667  [1]
 LDCM0466  CL73 HEK-293T C374(0.99); C165(1.19)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C165(0.97)  LDD1670  [1]
 LDCM0469  CL76 HEK-293T C165(1.02)  LDD1672  [1]
 LDCM0470  CL77 HEK-293T C374(1.15); C402(0.97); C165(1.41)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C374(1.03); C402(1.06)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C374(0.92); C165(0.94)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C165(1.68)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C165(1.17)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C165(1.22)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C165(1.08)  LDD1679  [1]
 LDCM0477  CL83 HEK-293T C374(1.14); C165(1.32)  LDD1680  [1]
 LDCM0479  CL85 HEK-293T C374(0.96); C165(1.04)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C165(1.14)  LDD1683  [1]
 LDCM0481  CL87 HEK-293T C165(0.97)  LDD1684  [1]
 LDCM0482  CL88 HEK-293T C165(1.11)  LDD1685  [1]
 LDCM0483  CL89 HEK-293T C374(1.10); C402(1.04); C165(1.05)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C165(1.19)  LDD1687  [1]
 LDCM0485  CL90 HEK-293T C374(1.01); C402(0.90)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C374(1.12); C165(0.75)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C165(1.44)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C165(1.08)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C165(1.16)  LDD1692  [1]
 LDCM0490  CL95 HEK-293T C374(1.01); C165(1.60)  LDD1693  [1]
 LDCM0492  CL97 HEK-293T C374(0.86); C165(1.11)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C165(1.02)  LDD1696  [1]
 LDCM0494  CL99 HEK-293T C165(1.06)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C165(1.03)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C165(1.11)  LDD1671  [1]
 LDCM0427  Fragment51 HEK-293T C165(0.93)  LDD1631  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Spastin (SPAST) AAA ATPase family Q9UBP0
Ras-related protein Rab-11A (RAB11A) Rab family P62491
Atlastin-1 (ATL1) GB1/RHD3 GTPase family Q8WXF7
Atlastin-2 (ATL2) GB1/RHD3 GTPase family Q8NHH9
Atlastin-3 (ATL3) GB1/RHD3 GTPase family Q6DD88
GTPase IMAP family member 1 (GIMAP1) AIG1/Toc34/Toc159-like paraseptin GTPase family Q8WWP7
GTPase IMAP family member 5 (GIMAP5) AIG1/Toc34/Toc159-like paraseptin GTPase family Q96F15
Peptidyl-prolyl cis-trans isomerase FKBP8 (FKBP8) . Q14318
Phosphatidylinositol-3-phosphatase SAC1 (SACM1L) . Q9NTJ5
Type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase (PIP4P2) . Q8N4L2
Transporter and channel
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proton-coupled zinc antiporter SLC30A8 (SLC30A8) Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family Q8IWU4
Receptor expression-enhancing protein 1 (REEP1) DP1 family Q9H902
Proton channel OTOP3 (OTOP3) Otopetrin family Q7RTS5
Transmembrane protein 14C (TMEM14C) TMEM14 family Q9P0S9
Vesicle-associated membrane protein-associated protein A (VAPA) VAMP-associated protein (VAP) (TC 9.B.17) family Q9P0L0
Vesicle-associated membrane protein-associated protein B/C (VAPB) VAMP-associated protein (VAP) (TC 9.B.17) family O95292
Protrudin (ZFYVE27) . Q5T4F4
Solute carrier family 66 member 2 (SLC66A2) . Q8N2U9
Transmembrane protein 65 (TMEM65) . Q6PI78
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Receptor expression-enhancing protein 5 (REEP5) DP1 family Q00765
ORM1-like protein 1 (ORMDL1) ORM family Q9P0S3
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7
Syntaxin-7 (STX7) Syntaxin family O15400
Tetraspanin-2 (TSPAN2) Tetraspanin (TM4SF) family O60636
Transmembrane protein 243 (TMEM243) TMEM243 family Q9BU79
Protein YIF1A (YIF1A) YIF1 family O95070
Immediate early response 3-interacting protein 1 (IER3IP1) YOS1 family Q9Y5U9

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019