Details of the Target

General Information of Target

Target ID LDTP06767
Target Name Protein Aster-B (GRAMD1B)
Gene Name GRAMD1B
Gene ID 57476
Synonyms
KIAA1201; Protein Aster-B; GRAM domain-containing protein 1B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKGFKLSCTASNSNRSTPACSPILRKRSRSPTPQNQDGDTMVEKGSDHSSDKSPSTPEQG
VQRSCSSQSGRSGGKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDTERLIVDYSCALQ
RDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEK
HFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDD
DFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFD
GLPLEEEALEGDGSLEKELAIDNIMGEKIEMIAPVNSPSLDFNDNEDIPTELSDSSDTHD
EGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKEE
NGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYT
INRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTES
TYLAEMHRQSPKEKASKTTTVRRRKRPHAHLRVPHLEEVMSPVTTPTDEDVGHRIKHVAG
STQTRHIPEDTPNGFHLQSVSKLLLVISCVICFSLVLLVILNMMLFYKLWMLEYTTQTLT
AWQGLRLQERLPQSQTEWAQLLESQQKYHDTELQKWREIIKSSVMLLDQMKDSLINLQNG
IRSRDYTSESEEKRNRYH
Target Bioclass
Transporter and channel
Subcellular location
Endoplasmic reticulum membrane
Function
Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER. Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol. In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain. At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER.
Uniprot ID
Q3KR37
Ensemble ID
ENST00000450171.2
HGNC ID
HGNC:29214

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C65(1.08)  LDD3323  [2]
Acrolein
 Probe Info  		N.A.  LDD0224  [3]
IA-alkyne
 Probe Info  		C157(0.00); C65(0.00)  LDD0162  [4]
NAIA_4
 Probe Info  		N.A.  LDD2226  [5]
WYneN
 Probe Info  		N.A.  LDD0021  [6]
IPM
 Probe Info  		C20(0.00); C65(0.00)  LDD0147  [7]
TFBX
 Probe Info  		C20(0.00); C65(0.00)  LDD0148  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C65(0.93)  LDD1507  [8]
 LDCM0226  AC11 HEK-293T C20(0.99)  LDD1509  [8]
 LDCM0259  AC14 HEK-293T C65(0.96)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C65(0.99)  LDD1513  [8]
 LDCM0276  AC17 HEK-293T C65(1.00)  LDD1515  [8]
 LDCM0278  AC19 HEK-293T C20(0.95)  LDD1517  [8]
 LDCM0281  AC21 HEK-293T C65(0.95)  LDD1520  [8]
 LDCM0282  AC22 HEK-293T C65(1.10)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C65(0.98)  LDD1522  [8]
 LDCM0284  AC24 HEK-293T C65(1.06)  LDD1523  [8]
 LDCM0285  AC25 HEK-293T C65(0.95)  LDD1524  [8]
 LDCM0287  AC27 HEK-293T C20(1.05)  LDD1526  [8]
 LDCM0289  AC29 HEK-293T C65(1.07)  LDD1528  [8]
 LDCM0290  AC3 HEK-293T C20(0.99)  LDD1529  [8]
 LDCM0291  AC30 HEK-293T C65(1.06)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C65(1.02)  LDD1531  [8]
 LDCM0293  AC32 HEK-293T C65(1.28)  LDD1532  [8]
 LDCM0294  AC33 HEK-293T C65(1.11)  LDD1533  [8]
 LDCM0296  AC35 HEK-293T C20(0.91)  LDD1535  [8]
 LDCM0298  AC37 HEK-293T C65(1.01)  LDD1537  [8]
 LDCM0299  AC38 HEK-293T C65(1.23)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C65(1.15)  LDD1539  [8]
 LDCM0302  AC40 HEK-293T C65(0.91)  LDD1541  [8]
 LDCM0303  AC41 HEK-293T C65(1.01)  LDD1542  [8]
 LDCM0305  AC43 HEK-293T C20(1.00)  LDD1544  [8]
 LDCM0307  AC45 HEK-293T C65(1.04)  LDD1546  [8]
 LDCM0308  AC46 HEK-293T C65(0.82)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C65(1.04)  LDD1548  [8]
 LDCM0310  AC48 HEK-293T C65(0.99)  LDD1549  [8]
 LDCM0311  AC49 HEK-293T C65(1.07)  LDD1550  [8]
 LDCM0312  AC5 HEK-293T C65(1.01)  LDD1551  [8]
 LDCM0314  AC51 HEK-293T C20(1.08)  LDD1553  [8]
 LDCM0316  AC53 HEK-293T C65(0.92)  LDD1555  [8]
 LDCM0317  AC54 HEK-293T C65(1.10)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C65(1.04)  LDD1557  [8]
 LDCM0319  AC56 HEK-293T C65(1.02)  LDD1558  [8]
 LDCM0320  AC57 HEK-293T C65(1.06)  LDD1559  [8]
 LDCM0322  AC59 HEK-293T C20(1.05)  LDD1561  [8]
 LDCM0323  AC6 HEK-293T C65(1.01)  LDD1562  [8]
 LDCM0325  AC61 HEK-293T C65(0.98)  LDD1564  [8]
 LDCM0326  AC62 HEK-293T C65(0.93)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C65(1.05)  LDD1566  [8]
 LDCM0328  AC64 HEK-293T C65(1.09)  LDD1567  [8]
 LDCM0334  AC7 HEK-293T C65(0.95)  LDD1568  [8]
 LDCM0345  AC8 HEK-293T C65(1.07)  LDD1569  [8]
 LDCM0248  AKOS034007472 HEK-293T C65(0.96)  LDD1511  [8]
 LDCM0356  AKOS034007680 HEK-293T C65(1.03)  LDD1570  [8]
 LDCM0275  AKOS034007705 HEK-293T C65(1.03)  LDD1514  [8]
 LDCM0367  CL1 HEK-293T C20(1.08)  LDD1571  [8]
 LDCM0368  CL10 HEK-293T C65(1.02)  LDD1572  [8]
 LDCM0370  CL101 HEK-293T C20(1.03)  LDD1574  [8]
 LDCM0372  CL103 HEK-293T C65(0.98)  LDD1576  [8]
 LDCM0374  CL105 HEK-293T C20(1.07)  LDD1578  [8]
 LDCM0376  CL107 HEK-293T C65(0.91)  LDD1580  [8]
 LDCM0378  CL109 HEK-293T C20(1.07)  LDD1582  [8]
 LDCM0379  CL11 HEK-293T C65(0.91)  LDD1583  [8]
 LDCM0381  CL111 HEK-293T C65(1.00)  LDD1585  [8]
 LDCM0383  CL113 HEK-293T C20(1.03)  LDD1587  [8]
 LDCM0385  CL115 HEK-293T C65(0.94)  LDD1589  [8]
 LDCM0387  CL117 HEK-293T C20(1.11)  LDD1591  [8]
 LDCM0389  CL119 HEK-293T C65(0.98)  LDD1593  [8]
 LDCM0390  CL12 HEK-293T C65(0.92)  LDD1594  [8]
 LDCM0392  CL121 HEK-293T C20(1.09)  LDD1596  [8]
 LDCM0394  CL123 HEK-293T C65(0.85)  LDD1598  [8]
 LDCM0396  CL125 HEK-293T C20(1.04)  LDD1600  [8]
 LDCM0398  CL127 HEK-293T C65(0.98)  LDD1602  [8]
 LDCM0400  CL13 HEK-293T C20(1.09)  LDD1604  [8]
 LDCM0402  CL15 HEK-293T C65(1.06)  LDD1606  [8]
 LDCM0404  CL17 HEK-293T C65(1.05)  LDD1608  [8]
 LDCM0406  CL19 HEK-293T C20(1.15)  LDD1610  [8]
 LDCM0409  CL21 HEK-293T C65(1.16)  LDD1613  [8]
 LDCM0410  CL22 HEK-293T C65(0.90)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C65(1.03)  LDD1615  [8]
 LDCM0412  CL24 HEK-293T C65(0.95)  LDD1616  [8]
 LDCM0413  CL25 HEK-293T C20(1.07)  LDD1617  [8]
 LDCM0415  CL27 HEK-293T C65(0.94)  LDD1619  [8]
 LDCM0417  CL29 HEK-293T C65(0.88)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C65(1.00)  LDD1622  [8]
 LDCM0420  CL31 HEK-293T C20(0.96)  LDD1624  [8]
 LDCM0422  CL33 HEK-293T C65(0.84)  LDD1626  [8]
 LDCM0423  CL34 HEK-293T C65(0.85)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C65(1.03)  LDD1628  [8]
 LDCM0425  CL36 HEK-293T C65(0.97)  LDD1629  [8]
 LDCM0426  CL37 HEK-293T C20(1.01)  LDD1630  [8]
 LDCM0428  CL39 HEK-293T C65(0.98)  LDD1632  [8]
 LDCM0431  CL41 HEK-293T C65(0.99)  LDD1635  [8]
 LDCM0433  CL43 HEK-293T C20(1.01)  LDD1637  [8]
 LDCM0435  CL45 HEK-293T C65(1.07)  LDD1639  [8]
 LDCM0436  CL46 HEK-293T C65(1.25)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C65(0.97)  LDD1641  [8]
 LDCM0438  CL48 HEK-293T C65(0.88)  LDD1642  [8]
 LDCM0439  CL49 HEK-293T C20(1.07)  LDD1643  [8]
 LDCM0440  CL5 HEK-293T C65(1.11)  LDD1644  [8]
 LDCM0444  CL53 HEK-293T C65(1.01)  LDD1647  [8]
 LDCM0446  CL55 HEK-293T C20(0.97)  LDD1649  [8]
 LDCM0448  CL57 HEK-293T C65(1.17)  LDD1651  [8]
 LDCM0449  CL58 HEK-293T C65(1.09)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C65(0.98)  LDD1653  [8]
 LDCM0452  CL60 HEK-293T C65(1.02)  LDD1655  [8]
 LDCM0453  CL61 HEK-293T C20(0.93)  LDD1656  [8]
 LDCM0455  CL63 HEK-293T C65(1.07)  LDD1658  [8]
 LDCM0457  CL65 HEK-293T C65(0.95)  LDD1660  [8]
 LDCM0459  CL67 HEK-293T C20(1.15)  LDD1662  [8]
 LDCM0461  CL69 HEK-293T C65(0.92)  LDD1664  [8]
 LDCM0462  CL7 HEK-293T C20(1.03)  LDD1665  [8]
 LDCM0463  CL70 HEK-293T C65(1.01)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C65(1.08)  LDD1667  [8]
 LDCM0465  CL72 HEK-293T C65(0.97)  LDD1668  [8]
 LDCM0466  CL73 HEK-293T C20(1.17)  LDD1669  [8]
 LDCM0470  CL77 HEK-293T C65(1.03)  LDD1673  [8]
 LDCM0472  CL79 HEK-293T C20(1.10)  LDD1675  [8]
 LDCM0475  CL81 HEK-293T C65(0.92)  LDD1678  [8]
 LDCM0476  CL82 HEK-293T C65(1.49)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C65(1.04)  LDD1680  [8]
 LDCM0478  CL84 HEK-293T C65(1.13)  LDD1681  [8]
 LDCM0479  CL85 HEK-293T C20(0.91)  LDD1682  [8]
 LDCM0481  CL87 HEK-293T C65(1.00)  LDD1684  [8]
 LDCM0483  CL89 HEK-293T C65(1.00)  LDD1686  [8]
 LDCM0484  CL9 HEK-293T C65(0.96)  LDD1687  [8]
 LDCM0486  CL91 HEK-293T C20(0.98)  LDD1689  [8]
 LDCM0488  CL93 HEK-293T C65(0.95)  LDD1691  [8]
 LDCM0489  CL94 HEK-293T C65(1.06)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C65(1.10)  LDD1693  [8]
 LDCM0491  CL96 HEK-293T C65(0.94)  LDD1694  [8]
 LDCM0492  CL97 HEK-293T C20(1.10)  LDD1695  [8]
 LDCM0494  CL99 HEK-293T C65(0.93)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C65(1.06)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C65(0.86)  LDD1671  [8]
 LDCM0022  KB02 T cell C210(7.29)  LDD1703  [9]
 LDCM0023  KB03 42-MG-BA C65(1.72)  LDD2661  [2]
 LDCM0024  KB05 SKMEL24 C65(1.08)  LDD3323  [2]
 LDCM0109  NEM HeLa N.A.  LDD0224  [3]

References

1 Labeling Preferences of Diazirines with Protein Biomolecules. J Am Chem Soc. 2021 May 5;143(17):6691-6700. doi: 10.1021/jacs.1c02509. Epub 2021 Apr 20.
Mass spectrometry data entry: PXD025140
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
9 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.