Details of the Target

General Information of Target

Target ID LDTP06717
Target Name E3 ubiquitin-protein ligase TRIM71 (TRIM71)
Gene Name TRIM71
Gene ID 131405
Synonyms
LIN41; E3 ubiquitin-protein ligase TRIM71; EC 2.3.2.27; Protein lin-41 homolog; RING-type E3 ubiquitin transferase TRIM71; Tripartite motif-containing protein 71
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGGGGGGPGAAARRLHVLP
CLHAFCRPCLEAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLL
DAVVATADEPPPKNGRAGAPAGAGGHSNHRHHAHHAHPRASASAPPLPQAPQPPAPSRSA
PGGPAASPSALLLRRPHGCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHYIER
GPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLGFCQHHDDEVLHLYCDTCSVPICRE
CTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVAEQVEMKAKVV
QSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQ
VLEEGRALDILLARDRMLAQVQELKTVRSLLQPQEDDRVMFTPPDQALYLAIKSFGFVSS
GAFAPLTKATGDGLKRALQGKVASFTVIGYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEV
SDQQNGTYVVSYRPQLEGEHLVSVTLCNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEG
DSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGAFHHKFGTLGSRPGQFDRPAGVA
CDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTR
NHRIQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQS
ARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQ
MDRPSGIAITPDGMIVVVDFGNNRILVF
Target Bioclass
Enzyme
Family
TRIM/RBCC family
Subcellular location
Cytoplasm, P-body
Function
E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (Probable). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression.
Uniprot ID
Q2Q1W2
Ensemble ID
ENST00000383763.6
HGNC ID
HGNC:32669

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K581(4.35)  LDD0277  [2]
IPM
 Probe Info  		C380(0.00); C69(0.00)  LDD0241  [3]
DBIA
 Probe Info  		C661(1.53)  LDD3383  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C607(1.15)  LDD1509  [5]
 LDCM0259  AC14 HEK-293T C607(0.94)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C607(1.28)  LDD1513  [5]
 LDCM0278  AC19 HEK-293T C607(0.96)  LDD1517  [5]
 LDCM0282  AC22 HEK-293T C607(1.02)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C607(1.17)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C91(0.91)  LDD1523  [5]
 LDCM0287  AC27 HEK-293T C607(1.12)  LDD1526  [5]
 LDCM0290  AC3 HEK-293T C607(1.11)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C607(1.06)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C607(1.27)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C91(0.78)  LDD1532  [5]
 LDCM0296  AC35 HEK-293T C607(1.10)  LDD1535  [5]
 LDCM0299  AC38 HEK-293T C607(0.98)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C607(1.27)  LDD1539  [5]
 LDCM0302  AC40 HEK-293T C91(0.82)  LDD1541  [5]
 LDCM0305  AC43 HEK-293T C607(1.14)  LDD1544  [5]
 LDCM0308  AC46 HEK-293T C607(1.00)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C607(1.40)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C91(0.70)  LDD1549  [5]
 LDCM0314  AC51 HEK-293T C607(1.12)  LDD1553  [5]
 LDCM0317  AC54 HEK-293T C607(1.01)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C607(1.19)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C91(0.79)  LDD1558  [5]
 LDCM0322  AC59 HEK-293T C607(1.08)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C607(0.99)  LDD1562  [5]
 LDCM0326  AC62 HEK-293T C607(0.90)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C607(1.18)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C91(0.88)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C607(1.42)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C91(1.10)  LDD1569  [5]
 LDCM0275  AKOS034007705 HEK-293T C91(0.71)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C202(1.05)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C607(0.62)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C202(0.83)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C607(1.07)  LDD1575  [5]
 LDCM0374  CL105 HEK-293T C202(1.02)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C607(1.22)  LDD1579  [5]
 LDCM0378  CL109 HEK-293T C202(1.11)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C607(1.06)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C607(1.06)  LDD1584  [5]
 LDCM0383  CL113 HEK-293T C202(1.13)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C607(0.98)  LDD1588  [5]
 LDCM0387  CL117 HEK-293T C202(0.97)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C607(0.94)  LDD1592  [5]
 LDCM0390  CL12 HEK-293T C91(1.18)  LDD1594  [5]
 LDCM0392  CL121 HEK-293T C202(0.85)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C607(1.07)  LDD1597  [5]
 LDCM0396  CL125 HEK-293T C202(1.01)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C607(1.14)  LDD1601  [5]
 LDCM0400  CL13 HEK-293T C202(0.71)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C607(0.89)  LDD1605  [5]
 LDCM0406  CL19 HEK-293T C607(1.01)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C607(1.07)  LDD1611  [5]
 LDCM0410  CL22 HEK-293T C607(0.77)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C607(1.08)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C91(1.09)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C202(0.85)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C607(1.16)  LDD1618  [5]
 LDCM0420  CL31 HEK-293T C607(0.97)  LDD1624  [5]
 LDCM0423  CL34 HEK-293T C607(0.69)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C607(0.95)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C91(0.93)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C202(0.93)  LDD1630  [5]
 LDCM0433  CL43 HEK-293T C607(1.01)  LDD1637  [5]
 LDCM0436  CL46 HEK-293T C607(0.77)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C607(1.03)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C91(1.24)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C202(0.90)  LDD1643  [5]
 LDCM0441  CL50 HEK-293T C607(1.10)  LDD1645  [5]
 LDCM0446  CL55 HEK-293T C607(0.96)  LDD1649  [5]
 LDCM0449  CL58 HEK-293T C607(0.74)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C607(1.15)  LDD1653  [5]
 LDCM0452  CL60 HEK-293T C91(1.09)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C202(1.13)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C607(1.30)  LDD1657  [5]
 LDCM0459  CL67 HEK-293T C607(1.01)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C607(0.93)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C607(0.74)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C607(1.05)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C91(0.99)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C202(1.08)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C607(1.24)  LDD1670  [5]
 LDCM0472  CL79 HEK-293T C607(1.06)  LDD1675  [5]
 LDCM0476  CL82 HEK-293T C607(0.81)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C607(1.06)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C91(0.89)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C202(0.83)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C607(1.07)  LDD1683  [5]
 LDCM0486  CL91 HEK-293T C607(1.01)  LDD1689  [5]
 LDCM0489  CL94 HEK-293T C607(0.81)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C607(1.00)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C91(1.28)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C202(0.95)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C607(1.00)  LDD1696  [5]
 LDCM0427  Fragment51 HEK-293T C607(1.42)  LDD1631  [5]
 LDCM0022  KB02 A2780 C661(2.42)  LDD2254  [4]
 LDCM0023  KB03 A2780 C661(3.38)  LDD2671  [4]
 LDCM0024  KB05 OVCAR-5 C661(1.53)  LDD3383  [4]

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402