Details of the Target

General Information of Target

Target ID LDTP06290
Target Name Josephin-1 (JOSD1)
Gene Name JOSD1
Gene ID 9929
Synonyms
JSPH1; KIAA0063; Josephin-1; EC 3.4.19.12; Josephin domain-containing protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSCVPWKGDKAKSESLELPQAAPPQIYHEKQRRELCALHALNNVFQDSNAFTRDTLQEIF
QRLSPNTMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIM
NLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGK
NCELLLVVPEEVEAHQSWRTDV
Target Bioclass
Enzyme
Subcellular location
Cell membrane
Function
Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.
Uniprot ID
Q15040
Ensemble ID
ENST00000216039.9
HGNC ID
HGNC:28953
ChEMBL ID
CHEMBL4630825

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C142(1.16)  LDD0625  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
IPM
 Probe Info  		N.A.  LDD0147  [4]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]
AOyne
 Probe Info  		11.50  LDD0443  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C36(1.07)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C36(1.12)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C36(0.97); C142(1.26)  LDD1509  [7]
 LDCM0237  AC12 HEK-293T C36(1.08)  LDD1510  [7]
 LDCM0276  AC17 HEK-293T C36(1.08)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C36(1.01)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C36(0.97); C142(1.49)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C36(1.04)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C36(0.86)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C36(1.04)  LDD1520  [7]
 LDCM0284  AC24 HEK-293T C36(0.98)  LDD1523  [7]
 LDCM0285  AC25 HEK-293T C36(1.06)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C36(1.08)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C36(0.93); C142(1.48)  LDD1526  [7]
 LDCM0288  AC28 HEK-293T C36(1.16)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C36(1.10)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C36(1.08); C142(1.60)  LDD1529  [7]
 LDCM0293  AC32 HEK-293T C36(0.96)  LDD1532  [7]
 LDCM0294  AC33 HEK-293T C36(0.98)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C36(1.03)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C36(0.90); C142(1.41)  LDD1535  [7]
 LDCM0297  AC36 HEK-293T C36(1.21)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C36(1.13)  LDD1537  [7]
 LDCM0301  AC4 HEK-293T C36(1.11)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C36(1.16)  LDD1541  [7]
 LDCM0303  AC41 HEK-293T C36(1.15)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C36(1.08)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C36(0.94); C142(1.56)  LDD1544  [7]
 LDCM0306  AC44 HEK-293T C36(1.22)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C36(1.21)  LDD1546  [7]
 LDCM0308  AC46 HCT 116 C142(1.16)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C142(0.98)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C142(1.16)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C142(1.09)  LDD0628  [1]
 LDCM0312  AC5 HEK-293T C36(1.07)  LDD1551  [7]
 LDCM0313  AC50 HCT 116 C142(1.10)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C142(1.20)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C142(0.99)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C142(0.98)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C142(0.98)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C142(1.05)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C142(1.33)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C142(1.05)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C142(1.20)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C142(1.20)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C142(1.12)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C142(1.08)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C142(1.20)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C142(1.00)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C142(1.09)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C142(1.50)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C142(1.52)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C142(1.37)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C142(1.09)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C142(0.88)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C142(0.95)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C142(1.15)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C142(0.77)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C142(0.95)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C142(0.91)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C142(1.00)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C142(0.95)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C142(0.91)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C142(1.06)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C142(0.83)  LDD0661  [1]
 LDCM0345  AC8 HEK-293T C36(1.16)  LDD1569  [7]
 LDCM0346  AC80 HCT 116 C142(0.87)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C142(0.89)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C142(1.20)  LDD0665  [1]
 LDCM0248  AKOS034007472 HEK-293T C36(1.29)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C36(1.04)  LDD1570  [7]
 LDCM0275  AKOS034007705 HEK-293T C36(1.06)  LDD1514  [7]
 LDCM0367  CL1 HEK-293T C182(0.77); C36(1.19)  LDD1571  [7]
 LDCM0369  CL100 HEK-293T C36(1.08)  LDD1573  [7]
 LDCM0370  CL101 HEK-293T C182(0.88); C36(0.99)  LDD1574  [7]
 LDCM0371  CL102 HEK-293T C36(1.37)  LDD1575  [7]
 LDCM0372  CL103 HEK-293T C182(1.56); C36(1.01)  LDD1576  [7]
 LDCM0373  CL104 HEK-293T C36(0.90)  LDD1577  [7]
 LDCM0374  CL105 HEK-293T C182(0.79); C36(1.12)  LDD1578  [7]
 LDCM0375  CL106 HEK-293T C36(1.06)  LDD1579  [7]
 LDCM0376  CL107 HEK-293T C182(1.20); C36(1.03)  LDD1580  [7]
 LDCM0377  CL108 HEK-293T C36(1.18)  LDD1581  [7]
 LDCM0378  CL109 HEK-293T C182(0.93); C36(1.02)  LDD1582  [7]
 LDCM0380  CL110 HEK-293T C36(1.82)  LDD1584  [7]
 LDCM0381  CL111 HEK-293T C182(1.00); C36(1.25)  LDD1585  [7]
 LDCM0382  CL112 HEK-293T C36(1.28)  LDD1586  [7]
 LDCM0383  CL113 HEK-293T C182(1.00); C36(0.84)  LDD1587  [7]
 LDCM0384  CL114 HEK-293T C36(1.70)  LDD1588  [7]
 LDCM0385  CL115 HEK-293T C182(1.14); C36(1.29)  LDD1589  [7]
 LDCM0386  CL116 HEK-293T C36(1.05)  LDD1590  [7]
 LDCM0387  CL117 HEK-293T C182(0.91); C36(1.10)  LDD1591  [7]
 LDCM0388  CL118 HEK-293T C36(1.14)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C182(1.71); C36(1.04)  LDD1593  [7]
 LDCM0390  CL12 HEK-293T C36(1.20)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C36(0.90)  LDD1595  [7]
 LDCM0392  CL121 HCT 116 C142(0.93)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C142(1.05)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C142(0.87)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C142(0.93)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C142(1.00)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C142(1.18)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C142(1.06)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C142(1.09)  LDD0716  [1]
 LDCM0400  CL13 HEK-293T C182(0.89); C36(1.16)  LDD1604  [7]
 LDCM0401  CL14 HEK-293T C36(1.22)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C182(1.28); C36(1.63)  LDD1606  [7]
 LDCM0403  CL16 HCT 116 C142(0.72)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C142(0.80)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C142(0.84)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C142(0.89)  LDD0723  [1]
 LDCM0407  CL2 HEK-293T C36(1.04)  LDD1611  [7]
 LDCM0408  CL20 HCT 116 C142(0.93)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C142(0.80)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C142(0.96)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C142(0.82)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C142(0.83)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C142(0.65)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C142(0.82)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C142(0.87)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C142(0.67)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C142(0.85)  LDD0734  [1]
 LDCM0418  CL3 HEK-293T C182(0.96); C36(1.40)  LDD1622  [7]
 LDCM0419  CL30 HCT 116 C142(0.73)  LDD0736  [1]
 LDCM0420  CL31 HEK-293T C36(1.33); C142(1.59)  LDD1624  [7]
 LDCM0421  CL32 HEK-293T C36(1.11)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C36(1.40)  LDD1626  [7]
 LDCM0425  CL36 HEK-293T C36(1.32)  LDD1629  [7]
 LDCM0426  CL37 HEK-293T C182(0.97); C36(1.37)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C182(1.07); C36(1.46)  LDD1632  [7]
 LDCM0429  CL4 HEK-293T C36(1.39)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C36(1.04)  LDD1634  [7]
 LDCM0431  CL41 HEK-293T C36(1.07)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C36(1.03)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C36(0.91); C142(1.26)  LDD1637  [7]
 LDCM0434  CL44 HEK-293T C36(0.96)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C36(1.39)  LDD1639  [7]
 LDCM0438  CL48 HEK-293T C36(1.18)  LDD1642  [7]
 LDCM0439  CL49 HEK-293T C182(0.98); C36(1.24)  LDD1643  [7]
 LDCM0440  CL5 HEK-293T C36(1.13)  LDD1644  [7]
 LDCM0441  CL50 HEK-293T C36(1.37)  LDD1645  [7]
 LDCM0443  CL52 HEK-293T C36(1.17)  LDD1646  [7]
 LDCM0444  CL53 HEK-293T C36(1.29)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C36(1.79)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C36(0.89); C142(1.54)  LDD1649  [7]
 LDCM0447  CL56 HEK-293T C36(1.56)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C36(1.25)  LDD1651  [7]
 LDCM0451  CL6 HEK-293T C36(1.54)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C36(1.20)  LDD1655  [7]
 LDCM0453  CL61 HEK-293T C182(0.88); C36(0.95)  LDD1656  [7]
 LDCM0454  CL62 HEK-293T C36(1.15)  LDD1657  [7]
 LDCM0455  CL63 HEK-293T C182(1.15); C36(1.03)  LDD1658  [7]
 LDCM0456  CL64 HEK-293T C36(1.13)  LDD1659  [7]
 LDCM0457  CL65 HEK-293T C36(1.04)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C36(1.26)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C36(1.52); C142(1.63)  LDD1662  [7]
 LDCM0460  CL68 HEK-293T C36(1.14)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C36(1.21)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C36(1.05); C142(1.72)  LDD1665  [7]
 LDCM0465  CL72 HEK-293T C36(1.19)  LDD1668  [7]
 LDCM0466  CL73 HEK-293T C182(1.13); C36(1.77)  LDD1669  [7]
 LDCM0467  CL74 HEK-293T C36(1.14)  LDD1670  [7]
 LDCM0469  CL76 HEK-293T C36(0.92)  LDD1672  [7]
 LDCM0470  CL77 HEK-293T C36(1.81)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C36(1.05)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C36(1.03); C142(1.57)  LDD1675  [7]
 LDCM0473  CL8 HEK-293T C36(1.00)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C36(1.13)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C36(1.27)  LDD1678  [7]
 LDCM0478  CL84 HEK-293T C36(1.47)  LDD1681  [7]
 LDCM0479  CL85 HEK-293T C182(0.64); C36(1.19)  LDD1682  [7]
 LDCM0480  CL86 HEK-293T C36(1.33)  LDD1683  [7]
 LDCM0481  CL87 HEK-293T C182(1.57); C36(1.33)  LDD1684  [7]
 LDCM0482  CL88 HEK-293T C36(1.38)  LDD1685  [7]
 LDCM0483  CL89 HEK-293T C36(1.22)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C36(1.25)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C36(2.36)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C36(1.11); C142(1.60)  LDD1689  [7]
 LDCM0487  CL92 HEK-293T C36(1.32)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C36(1.25)  LDD1691  [7]
 LDCM0491  CL96 HEK-293T C36(1.38)  LDD1694  [7]
 LDCM0492  CL97 HEK-293T C182(0.67); C36(1.15)  LDD1695  [7]
 LDCM0493  CL98 HEK-293T C36(1.29)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C182(1.25); C36(1.27)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C182(1.01); C36(1.22)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C182(1.46); C36(1.26)  LDD1671  [7]
 LDCM0427  Fragment51 HEK-293T C36(1.08)  LDD1631  [7]
 LDCM0022  KB02 T cell-activated C36(16.88)  LDD1704  [8]
 LDCM0023  KB03 CC-LP-1 C36(2.82)  LDD2715  [9]
 LDCM0024  KB05 SG231 C36(3.36)  LDD3418  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
Protein-glutamine gamma-glutamyltransferase K (TGM1) Transglutaminase family P22735
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MyoD family inhibitor (MDFI) MDFI family Q99750
Other
Click To Hide/Show 20 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Fibulin-2 (FBLN2) Fibulin family P98095
Keratin, type I cuticular Ha1 (KRT31) Intermediate filament family Q15323
Keratin, type I cuticular Ha4 (KRT34) Intermediate filament family O76011
Keratin, type I cytoskeletal 27 (KRT27) Intermediate filament family Q7Z3Y8
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
Keratin, type II cuticular Hb6 (KRT86) Intermediate filament family O43790
Keratin-associated protein 1-1 (KRTAP1-1) KRTAP type 1 family Q07627
Keratin-associated protein 1-3 (KRTAP1-3) KRTAP type 1 family Q8IUG1
Keratin-associated protein 10-4 (KRTAP10-4) KRTAP type 10 family P60372
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410
Keratin-associated protein 12-3 (KRTAP12-3) KRTAP type 12 family P60328
Keratin-associated protein 2-4 (KRTAP2-4) KRTAP type 2 family Q9BYR9
Keratin-associated protein 5-9 (KRTAP5-9) KRTAP type 5 family P26371
Keratin-associated protein 9-2 (KRTAP9-2) KRTAP type 9 family Q9BYQ4
Keratin-associated protein 9-3 (KRTAP9-3) KRTAP type 9 family Q9BYQ3
Notch homolog 2 N-terminal-like protein A (NOTCH2NLA) NOTCH family Q7Z3S9
Notch homolog 2 N-terminal-like protein C (NOTCH2NLC) NOTCH family P0DPK4
Caspase recruitment domain-containing protein 10 (CARD10) . Q9BWT7
TNF receptor-associated factor 1 (TRAF1) . Q13077

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
9 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840