Details of the Target

General Information of Target

Target ID LDTP05983
Target Name Protein Shroom2 (SHROOM2)
Gene Name SHROOM2
Gene ID 357
Synonyms
APXL; Protein Shroom2; Apical-like protein; Protein APXL
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGAEPRARPERLAEAETRAADGGRLVEVQLSGGAPWGFTLKGGREHGEPLVITKIEEGS
KAAAVDKLLAGDEIVGINDIGLSGFRQEAICLVKGSHKTLKLVVKRRSELGWRPHSWHAT
KFSDSHPELAASPFTSTSGCPSWSGRHHASSSSHDLSSSWEQTNLQRTLDHFSSLGSVDS
LDHPSSRLSVAKSNSSIDHLGSHSKRDSAYGSFSTSSSTPDHTLSKADTSSAENILYTVG
LWEAPRQGGRQAQAAGDPQGSEEKLSCFPPRVPGDSGKGPRPEYNAEPKLAAPGRSNFGP
VWYVPDKKKAPSSPPPPPPPLRSDSFAATKSHEKAQGPVFSEAAAAQHFTALAQAQPRGD
RRPELTDRPWRSAHPGSLGKGSGGPGCPQEAHADGSWPPSKDGASSRLQASLSSSDVRFP
QSPHSGRHPPLYSDHSPLCADSLGQEPGAASFQNDSPPQVRGLSSCDQKLGSGWQGPRPC
VQGDLQAAQLWAGCWPSDTALGALESLPPPTVGQSPRHHLPQPEGPPDARETGRCYPLDK
GAEGCSAGAQEPPRASRAEKASQRLAASITWADGESSRICPQETPLLHSLTQEGKRRPES
SPEDSATRPPPFDAHVGKPTRRSDRFATTLRNEIQMHRAKLQKSRSTVALTAAGEAEDGT
GRWRAGLGGGTQEGPLAGTYKDHLKEAQARVLRATSFKRRDLDPNPGDLYPESLEHRMGD
PDTVPHFWEAGLAQPPSSTSGGPHPPRIGGRRRFTAEQKLKSYSEPEKMNEVGLTRGYSP
HQHPRTSEDTVGTFADRWKFFEETSKPVPQRPAQKQALHGIPRDKPERPRTAGRTCEGTE
PWSRTTSLGDSLNAHSAAEKAGTSDLPRRLGTFAEYQASWKEQRKPLEARSSGRCHSADD
ILDVSLDPQERPQHVHGRSRSSPSTDHYKQEASVELRRQAGDPGEPREELPSAVRAEEGQ
STPRQADAQCREGSPGSQQHPPSQKAPNPPTFSELSHCRGAPELPREGRGRAGTLPRDYR
YSEESTPADLGPRAQSPGSPLHARGQDSWPVSSALLSKRPAPQRPPPPKREPRRYRATDG
APADAPVGVLGRPFPTPSPASLDVYVARLSLSHSPSVFSSAQPQDTPKATVCERGSQHVS
GDASRPLPEALLPPKQQHLRLQTATMETSRSPSPQFAPQKLTDKPPLLIQDEDSTRIERV
MDNNTTVKMVPIKIVHSESQPEKESRQSLACPAEPPALPHGLEKDQIKTLSTSEQFYSRF
CLYTRQGAEPEAPHRAQPAEPQPLGTQVPPEKDRCTSPPGLSYMKAKEKTVEDLKSEELA
REIVGKDKSLADILDPSVKIKTTMDLMEGIFPKDEHLLEEAQQRRKLLPKIPSPRSTEER
KEEPSVPAAVSLATNSTYYSTSAPKAELLIKMKDLQEQQEHEEDSGSDLDHDLSVKKQEL
IESISRKLQVLREARESLLEDVQANTVLGAEVEAIVKGVCKPSEFDKFRMFIGDLDKVVN
LLLSLSGRLARVENALNNLDDGASPGDRQSLLEKQRVLIQQHEDAKELKENLDRRERIVF
DILANYLSEESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK
Target Bioclass
Other
Family
Shroom family
Subcellular location
Apical cell membrane
Function
May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.
Uniprot ID
Q13796
Ensemble ID
ENST00000380913.8
HGNC ID
HGNC:630

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y1021(8.77)  LDD3495  [1]
AHL-Pu-1
 Probe Info  		C970(2.83)  LDD0171  [2]
IPM
 Probe Info  		C1132(9.88)  LDD1702  [3]
DBIA
 Probe Info  		C1605(0.96)  LDD0531  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
NAIA_5
 Probe Info  		C545(0.00); C580(0.00); C91(0.00); C970(0.00)  LDD2224  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C970(2.83)  LDD0171  [2]
 LDCM0214  AC1 HCT 116 C1605(0.96)  LDD0531  [4]
 LDCM0215  AC10 HCT 116 C1605(1.26)  LDD0532  [4]
 LDCM0226  AC11 HCT 116 C1605(1.24)  LDD0543  [4]
 LDCM0237  AC12 HCT 116 C1605(1.22)  LDD0554  [4]
 LDCM0259  AC14 HCT 116 C1605(1.27)  LDD0576  [4]
 LDCM0270  AC15 HCT 116 C1605(1.47)  LDD0587  [4]
 LDCM0276  AC17 HCT 116 C1605(1.42)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C1605(1.41)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C1605(1.64)  LDD0595  [4]
 LDCM0279  AC2 HCT 116 C1605(0.91)  LDD0596  [4]
 LDCM0280  AC20 HCT 116 C1605(1.22)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C1605(1.28)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C1605(1.46)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C1605(1.18)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C1605(1.07)  LDD0601  [4]
 LDCM0290  AC3 HCT 116 C1605(1.00)  LDD0607  [4]
 LDCM0291  AC30 HEK-293T C91(0.88); C466(0.78)  LDD1530  [7]
 LDCM0296  AC35 HCT 116 C1605(0.76)  LDD0613  [4]
 LDCM0297  AC36 HCT 116 C1605(1.18)  LDD0614  [4]
 LDCM0298  AC37 HCT 116 C1605(0.85)  LDD0615  [4]
 LDCM0299  AC38 HCT 116 C1605(1.11)  LDD0616  [4]
 LDCM0300  AC39 HCT 116 C1605(1.11)  LDD0617  [4]
 LDCM0301  AC4 HCT 116 C1605(1.05)  LDD0618  [4]
 LDCM0302  AC40 HCT 116 C1605(0.92)  LDD0619  [4]
 LDCM0303  AC41 HCT 116 C1605(0.85)  LDD0620  [4]
 LDCM0304  AC42 HCT 116 C1605(0.90)  LDD0621  [4]
 LDCM0305  AC43 HCT 116 C1605(0.90)  LDD0622  [4]
 LDCM0306  AC44 HCT 116 C1605(0.88)  LDD0623  [4]
 LDCM0307  AC45 HCT 116 C1605(0.61)  LDD0624  [4]
 LDCM0308  AC46 HCT 116 C1605(0.98)  LDD0625  [4]
 LDCM0309  AC47 HCT 116 C1605(0.92)  LDD0626  [4]
 LDCM0310  AC48 HCT 116 C1605(1.02)  LDD0627  [4]
 LDCM0311  AC49 HCT 116 C1605(1.03)  LDD0628  [4]
 LDCM0312  AC5 HCT 116 C1605(0.98)  LDD0629  [4]
 LDCM0313  AC50 HCT 116 C1605(0.89)  LDD0630  [4]
 LDCM0314  AC51 HCT 116 C1605(1.01)  LDD0631  [4]
 LDCM0315  AC52 HCT 116 C1605(0.93)  LDD0632  [4]
 LDCM0316  AC53 HCT 116 C1605(0.92)  LDD0633  [4]
 LDCM0317  AC54 HCT 116 C1605(0.92)  LDD0634  [4]
 LDCM0318  AC55 HCT 116 C1605(1.24)  LDD0635  [4]
 LDCM0319  AC56 HCT 116 C1605(0.94)  LDD0636  [4]
 LDCM0320  AC57 HCT 116 C1605(2.09)  LDD0637  [4]
 LDCM0321  AC58 HCT 116 C1605(1.33)  LDD0638  [4]
 LDCM0322  AC59 HCT 116 C1605(1.54)  LDD0639  [4]
 LDCM0323  AC6 HCT 116 C1605(0.88)  LDD0640  [4]
 LDCM0324  AC60 HCT 116 C1605(2.28)  LDD0641  [4]
 LDCM0325  AC61 HCT 116 C1605(2.32)  LDD0642  [4]
 LDCM0326  AC62 HCT 116 C1605(1.89)  LDD0643  [4]
 LDCM0327  AC63 HCT 116 C1605(2.52)  LDD0644  [4]
 LDCM0328  AC64 HCT 116 C1605(2.07)  LDD0645  [4]
 LDCM0329  AC65 HCT 116 C1605(1.86)  LDD0646  [4]
 LDCM0330  AC66 HCT 116 C1605(0.79)  LDD0647  [4]
 LDCM0331  AC67 HCT 116 C1605(1.93)  LDD0648  [4]
 LDCM0334  AC7 HCT 116 C1605(1.09)  LDD0651  [4]
 LDCM0345  AC8 HCT 116 C1605(1.25)  LDD0662  [4]
 LDCM0248  AKOS034007472 HCT 116 C1605(1.05)  LDD0565  [4]
 LDCM0356  AKOS034007680 HCT 116 C1605(0.97)  LDD0673  [4]
 LDCM0275  AKOS034007705 HCT 116 C1605(1.18)  LDD0592  [4]
 LDCM0367  CL1 HCT 116 C1605(0.96)  LDD0684  [4]
 LDCM0368  CL10 HCT 116 C1605(0.79)  LDD0685  [4]
 LDCM0369  CL100 HCT 116 C1605(1.23)  LDD0686  [4]
 LDCM0370  CL101 HCT 116 C1605(1.23)  LDD0687  [4]
 LDCM0371  CL102 HCT 116 C1605(1.48)  LDD0688  [4]
 LDCM0372  CL103 HCT 116 C1605(1.49)  LDD0689  [4]
 LDCM0373  CL104 HCT 116 C1605(1.22)  LDD0690  [4]
 LDCM0374  CL105 HCT 116 C1605(1.34)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C1605(1.43)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C1605(1.63)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C1605(1.62)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C1605(1.34)  LDD0695  [4]
 LDCM0379  CL11 HCT 116 C1605(0.68)  LDD0696  [4]
 LDCM0380  CL110 HCT 116 C1605(1.16)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C1605(1.76)  LDD0698  [4]
 LDCM0383  CL113 HEK-293T C91(0.98)  LDD1587  [7]
 LDCM0387  CL117 HCT 116 C1605(1.19)  LDD0704  [4]
 LDCM0388  CL118 HCT 116 C1605(0.99)  LDD0705  [4]
 LDCM0389  CL119 HCT 116 C1605(1.03)  LDD0706  [4]
 LDCM0390  CL12 HCT 116 C1605(0.78)  LDD0707  [4]
 LDCM0391  CL120 HCT 116 C1605(1.02)  LDD0708  [4]
 LDCM0392  CL121 HCT 116 C1605(1.04)  LDD0709  [4]
 LDCM0393  CL122 HCT 116 C1605(0.99)  LDD0710  [4]
 LDCM0394  CL123 HCT 116 C1605(0.91)  LDD0711  [4]
 LDCM0395  CL124 HCT 116 C1605(1.10)  LDD0712  [4]
 LDCM0396  CL125 HCT 116 C1605(1.50)  LDD0713  [4]
 LDCM0397  CL126 HCT 116 C1605(1.10)  LDD0714  [4]
 LDCM0398  CL127 HCT 116 C1605(1.48)  LDD0715  [4]
 LDCM0399  CL128 HCT 116 C1605(2.08)  LDD0716  [4]
 LDCM0400  CL13 HCT 116 C1605(0.73)  LDD0717  [4]
 LDCM0401  CL14 HCT 116 C1605(0.71)  LDD0718  [4]
 LDCM0402  CL15 HCT 116 C1605(0.68)  LDD0719  [4]
 LDCM0403  CL16 HCT 116 C1605(1.15)  LDD0720  [4]
 LDCM0404  CL17 HCT 116 C1605(1.30)  LDD0721  [4]
 LDCM0405  CL18 HCT 116 C1605(1.69)  LDD0722  [4]
 LDCM0406  CL19 HCT 116 C1605(1.81)  LDD0723  [4]
 LDCM0407  CL2 HCT 116 C1605(0.77)  LDD0724  [4]
 LDCM0408  CL20 HCT 116 C1605(1.65)  LDD0725  [4]
 LDCM0409  CL21 HCT 116 C1605(2.11)  LDD0726  [4]
 LDCM0410  CL22 HCT 116 C1605(1.87)  LDD0727  [4]
 LDCM0411  CL23 HCT 116 C1605(0.97)  LDD0728  [4]
 LDCM0412  CL24 HCT 116 C1605(1.29)  LDD0729  [4]
 LDCM0413  CL25 HCT 116 C1605(1.21)  LDD0730  [4]
 LDCM0414  CL26 HCT 116 C1605(1.31)  LDD0731  [4]
 LDCM0415  CL27 HCT 116 C1605(1.21)  LDD0732  [4]
 LDCM0416  CL28 HCT 116 C1605(1.10)  LDD0733  [4]
 LDCM0417  CL29 HCT 116 C1605(1.04)  LDD0734  [4]
 LDCM0418  CL3 HCT 116 C1605(0.80)  LDD0735  [4]
 LDCM0419  CL30 HCT 116 C1605(1.22)  LDD0736  [4]
 LDCM0423  CL34 HEK-293T C91(5.27); C466(0.90)  LDD1627  [7]
 LDCM0426  CL37 HEK-293T C91(1.06)  LDD1630  [7]
 LDCM0429  CL4 HCT 116 C1605(0.91)  LDD0746  [4]
 LDCM0436  CL46 HEK-293T C91(4.89); C466(0.89)  LDD1640  [7]
 LDCM0439  CL49 HEK-293T C91(1.17)  LDD1643  [7]
 LDCM0440  CL5 HCT 116 C1605(0.78)  LDD0757  [4]
 LDCM0449  CL58 HEK-293T C91(5.90); C466(1.01)  LDD1652  [7]
 LDCM0451  CL6 HCT 116 C1605(0.84)  LDD0768  [4]
 LDCM0453  CL61 HEK-293T C91(1.06)  LDD1656  [7]
 LDCM0462  CL7 HCT 116 C1605(0.76)  LDD0779  [4]
 LDCM0463  CL70 HEK-293T C91(3.19); C466(0.95)  LDD1666  [7]
 LDCM0466  CL73 HEK-293T C91(1.23)  LDD1669  [7]
 LDCM0473  CL8 HCT 116 C1605(0.80)  LDD0790  [4]
 LDCM0476  CL82 HEK-293T C91(2.91); C466(1.00)  LDD1679  [7]
 LDCM0479  CL85 HEK-293T C91(1.29)  LDD1682  [7]
 LDCM0484  CL9 HCT 116 C1605(0.75)  LDD0801  [4]
 LDCM0486  CL91 HCT 116 C1605(0.94)  LDD0803  [4]
 LDCM0487  CL92 HCT 116 C1605(0.83)  LDD0804  [4]
 LDCM0488  CL93 HCT 116 C1605(0.90)  LDD0805  [4]
 LDCM0489  CL94 HCT 116 C1605(1.03)  LDD0806  [4]
 LDCM0490  CL95 HCT 116 C1605(0.74)  LDD0807  [4]
 LDCM0491  CL96 HCT 116 C1605(0.83)  LDD0808  [4]
 LDCM0492  CL97 HCT 116 C1605(0.96)  LDD0809  [4]
 LDCM0493  CL98 HCT 116 C1605(1.17)  LDD0810  [4]
 LDCM0494  CL99 HCT 116 C1605(0.82)  LDD0811  [4]
 LDCM0634  CY-0357 Hep-G2 C1605(0.58)  LDD2228  [6]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C1132(9.88)  LDD1702  [3]
 LDCM0022  KB02 A-375 C1261(4.31); C91(1.34)  LDD2255  [8]
 LDCM0023  KB03 AU565 C545(1.21)  LDD2682  [8]
 LDCM0024  KB05 MKN45 C1605(1.86)  LDD3332  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SH2/SH3 adapter protein NCK1 (NCK1) . P16333

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840