General Information of Target

Target ID LDTP05415
Target Name AT-rich interactive domain-containing protein 5A (ARID5A)
Gene Name ARID5A
Gene ID 10865
Synonyms
MRF1; AT-rich interactive domain-containing protein 5A; ARID domain-containing protein 5A; Modulator recognition factor 1; MRF-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPVKGNRKQSTEGDALDPPASPKPAGKQNGIQNPISLEDSPEAGGEREEEQEREEEQA
FLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGG
SPGSTSAATCTRRHYERLVLPYVRHLKGEDDKPLPTSKPRKQYKMAKENRGDDGATERPK
KAKEERRMDQMMPGKTKADAADPAPLPSQEPPRNSTEQQGLASGSSVSFVGASGCPEAYK
RLLSSFYCKGTHGIMSPLAKKKLLAQVSKVEALQCQEEGCRHGAEPQASPAVHLPESPQS
PKGLTENSRHRLTPQEGLQAPGGSLREEAQAGPCPAAPIFKGCFYTHPTEVLKPVSQHPR
DFFSRLKDGVLLGPPGKEGLSVKEPQLVWGGDANRPSAFHKGGSRKGILYPKPKACWVSP
MAKVPAESPTLPPTFPSSPGLGSKRSLEEEGAAHSGKRLRAVSPFLKEADAKKCGAKPAG
SGLVSCLLGPALGPVPPEAYRGTMLHCPLNFTGTPGPLKGQAALPFSPLVIPAFPAHFLA
TAGPSPMAAGLMHFPPTSFDSALRHRLCPASSAWHAPPVTTYAAPHFFHLNTKL
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison. May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention. Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors. As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock. Stabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR; thereby competing with the mRNA-destabilizing functions of RC3H1 and endoribonuclease ZC3H12A.
Uniprot ID
Q03989
Ensemble ID
ENST00000357485.8
HGNC ID
HGNC:17361

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO320 SNV: p.V424A .
HCT116 Deletion: p.G120AfsTer27 .
ICC12 SNV: p.G107R .
ICC9 SNV: p.R108C .
JURKAT Deletion: p.R213GfsTer55 .
LNCaP clone FGC SNV: p.R9M; p.T106I .
MDST8 SNV: p.F68L .
MEWO SNV: p.P535S .
MFE319 SNV: p.A336V; p.P338L .
RL SNV: p.G322R .
SKMEL30 SNV: p.H400Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info 
C130(4.43)  LDD0523  [1]
DBIA
 Probe Info 
C248(1.10)  LDD1509  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C248(1.10)  LDD1509  [2]
 LDCM0259  AC14 HEK-293T C416(0.81)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C248(0.93)  LDD1513  [2]
 LDCM0278  AC19 HEK-293T C248(1.10)  LDD1517  [2]
 LDCM0282  AC22 HEK-293T C416(0.80)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C248(1.09)  LDD1522  [2]
 LDCM0287  AC27 HEK-293T C248(1.25)  LDD1526  [2]
 LDCM0290  AC3 HEK-293T C248(1.11)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C416(0.83)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C248(1.04)  LDD1531  [2]
 LDCM0296  AC35 HEK-293T C248(1.18)  LDD1535  [2]
 LDCM0299  AC38 HEK-293T C416(0.65)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C248(0.75)  LDD1539  [2]
 LDCM0305  AC43 HEK-293T C248(1.26)  LDD1544  [2]
 LDCM0308  AC46 HEK-293T C416(0.70)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C248(0.77)  LDD1548  [2]
 LDCM0314  AC51 HEK-293T C248(1.23)  LDD1553  [2]
 LDCM0317  AC54 HEK-293T C416(0.69)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C248(0.95)  LDD1557  [2]
 LDCM0322  AC59 HEK-293T C248(1.08)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C416(0.81)  LDD1562  [2]
 LDCM0326  AC62 HEK-293T C416(0.73)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C248(1.04)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C248(0.87)  LDD1568  [2]
 LDCM0368  CL10 HEK-293T C416(0.87)  LDD1572  [2]
 LDCM0369  CL100 HEK-293T C248(1.06)  LDD1573  [2]
 LDCM0372  CL103 HEK-293T C248(1.12)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C248(1.28)  LDD1577  [2]
 LDCM0376  CL107 HEK-293T C248(1.15)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C248(1.26)  LDD1581  [2]
 LDCM0379  CL11 HEK-293T C248(1.15)  LDD1583  [2]
 LDCM0381  CL111 HEK-293T C248(1.15)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C248(0.98)  LDD1586  [2]
 LDCM0385  CL115 HEK-293T C248(1.14)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C248(1.04)  LDD1590  [2]
 LDCM0389  CL119 HEK-293T C248(0.97)  LDD1593  [2]
 LDCM0391  CL120 HEK-293T C248(1.15)  LDD1595  [2]
 LDCM0394  CL123 HEK-293T C248(0.86)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C248(1.05)  LDD1599  [2]
 LDCM0398  CL127 HEK-293T C248(1.11)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C248(1.11)  LDD1603  [2]
 LDCM0402  CL15 HEK-293T C248(1.06)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C248(1.11)  LDD1607  [2]
 LDCM0406  CL19 HEK-293T C248(1.13)  LDD1610  [2]
 LDCM0410  CL22 HEK-293T C416(0.79)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C248(1.03)  LDD1615  [2]
 LDCM0415  CL27 HEK-293T C248(0.92)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C248(1.04)  LDD1620  [2]
 LDCM0418  CL3 HEK-293T C248(1.25)  LDD1622  [2]
 LDCM0420  CL31 HEK-293T C248(1.06)  LDD1624  [2]
 LDCM0423  CL34 HEK-293T C416(0.80)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C248(0.88)  LDD1628  [2]
 LDCM0428  CL39 HEK-293T C248(1.08)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C248(1.07)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C248(1.19)  LDD1634  [2]
 LDCM0433  CL43 HEK-293T C248(0.89)  LDD1637  [2]
 LDCM0436  CL46 HEK-293T C416(0.73)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C248(1.01)  LDD1641  [2]
 LDCM0443  CL52 HEK-293T C248(1.17)  LDD1646  [2]
 LDCM0446  CL55 HEK-293T C248(1.01)  LDD1649  [2]
 LDCM0449  CL58 HEK-293T C416(0.80)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C248(0.91)  LDD1653  [2]
 LDCM0455  CL63 HEK-293T C248(1.17)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C248(1.23)  LDD1659  [2]
 LDCM0459  CL67 HEK-293T C248(1.36)  LDD1662  [2]
 LDCM0462  CL7 HEK-293T C248(0.95)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C416(0.69)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C248(0.97)  LDD1667  [2]
 LDCM0469  CL76 HEK-293T C248(0.95)  LDD1672  [2]
 LDCM0472  CL79 HEK-293T C248(1.11)  LDD1675  [2]
 LDCM0476  CL82 HEK-293T C416(0.67)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C248(0.97)  LDD1680  [2]
 LDCM0481  CL87 HEK-293T C248(1.05)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C248(0.93)  LDD1685  [2]
 LDCM0486  CL91 HEK-293T C248(1.07)  LDD1689  [2]
 LDCM0489  CL94 HEK-293T C416(0.78)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C248(1.24)  LDD1693  [2]
 LDCM0494  CL99 HEK-293T C248(1.08)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C248(1.32)  LDD1698  [2]
 LDCM0203  EV-96 T cell C130(4.43)  LDD0523  [1]
 LDCM0468  Fragment33 HEK-293T C248(1.13)  LDD1671  [2]
 LDCM0022  KB02 T cell-activated C507(20.00); C248(11.20); C334(13.11); C235(7.58)  LDD1704  [1]
 LDCM0024  KB05 T cell-activated C416(14.69); C507(11.28); C248(20.00); C334(20.00)  LDD1706  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 21 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
3 beta-hydroxysteroid dehydrogenase type 7 (HSD3B7) 3-beta-HSD family Q9H2F3
5'-AMP-activated protein kinase subunit beta-2 (PRKAB2) 5'-AMP-activated protein kinase beta subunit family O43741
Replication factor C subunit 5 (RFC5) Activator 1 small subunits family P40937
Histone-lysine N-methyltransferase SMYD1 (SMYD1) Class V-like SAM-binding methyltransferase superfamily Q8NB12
Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) Class V-like SAM-binding methyltransferase superfamily Q9NQX0
Probable E3 ubiquitin-protein ligase DTX2 (DTX2) Deltex family Q86UW9
Protein mab-21-like 2 (MAB21L2) Mab-21 family Q9Y586
Uridine diphosphate glucose pyrophosphatase NUDT22 (NUDT22) Nudix hydrolase family Q9BRQ3
Group 10 secretory phospholipase A2 (PLA2G10) Phospholipase A2 family O15496
Testis-specific serine/threonine-protein kinase 3 (TSSK3) CAMK Ser/Thr protein kinase family Q96PN8
Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) CAMK Ser/Thr protein kinase family Q9UQM7
Tyrosine-protein kinase transmembrane receptor ROR2 (ROR2) Tyr protein kinase family Q01974
Tensin-2 (TNS2) PTEN phosphatase protein family Q63HR2
Terminal nucleotidyltransferase 5D (TENT5D) TENT family Q8NEK8
Protein-glutamine gamma-glutamyltransferase Z (TGM7) Transglutaminase family Q96PF1
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
V-type proton ATPase subunit d 2 (ATP6V0D2) V-ATPase V0D/AC39 subunit family Q8N8Y2
E3 ubiquitin-protein ligase MYLIP (MYLIP) . Q8WY64
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Ubiquitin domain-containing protein 2 (UBTD2) . Q8WUN7
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cation channel sperm-associated protein 1 (CATSPER1) Cation channel sperm-associated (TC 1.A.1.19) family Q8NEC5
Cytochrome c oxidase subunit 5B, mitochondrial (COX5B) Cytochrome c oxidase subunit 5B family P10606
MyoD family inhibitor (MDFI) MDFI family Q99750
Transcription factor
Click To Hide/Show 18 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Zinc finger protein GLIS2 (GLIS2) GLI C2H2-type zinc-finger protein family Q9BZE0
Zinc finger protein ZIC 1 (ZIC1) GLI C2H2-type zinc-finger protein family Q15915
Zinc finger protein PLAGL2 (PLAGL2) Krueppel C2H2-type zinc-finger protein family Q9UPG8
Estrogen receptor (ESR1) Nuclear hormone receptor family P03372
Homeobox protein OTX1 (OTX1) Paired homeobox family P32242
Pituitary homeobox 1 (PITX1) Paired homeobox family P78337
PHD finger protein 1 (PHF1) Polycomblike family O43189
Pituitary-specific positive transcription factor 1 (POU1F1) POU transcription factor family P28069
Iroquois-class homeodomain protein IRX-6 (IRX6) TALE/IRO homeobox family P78412
AT-rich interactive domain-containing protein 5A (ARID5A) . Q03989
Forkhead box protein D2 (FOXD2) . O60548
Forkhead box protein H1 (FOXH1) . O75593
Homeobox protein VENTX (VENTX) . O95231
Oligodendrocyte transcription factor 3 (OLIG3) . Q7RTU3
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (SOHLH1) . Q5JUK2
Transcription factor GATA-5 (GATA5) . Q9BWX5
Transcriptional regulator QRICH1 (QRICH1) . Q2TAL8
Other
Click To Hide/Show 48 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Ataxin-1-like (ATXN1L) ATXN1 family P0C7T5
Beta-crystallin A2 (CRYBA2) Beta/gamma-crystallin family P53672
Beta-crystallin A3 (CRYBA1) Beta/gamma-crystallin family P05813
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) CDIP1/LITAF family Q99732
Cyclin-dependent kinases regulatory subunit 1 (CKS1B) CKS family P61024
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Docking protein 6 (DOK6) DOK family, Type B subfamily Q6PKX4
Zinc finger protein 503 (ZNF503) Elbow/Noc family Q96F45
Epithelial splicing regulatory protein 1 (ESRP1) ESRP family Q6NXG1
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410
Keratin-associated protein 12-2 (KRTAP12-2) KRTAP type 12 family P59991
Keratin-associated protein 19-3 (KRTAP19-3) KRTAP type 19 family Q7Z4W3
Keratin-associated protein 19-5 (KRTAP19-5) KRTAP type 19 family Q3LI72
Keratin-associated protein 19-7 (KRTAP19-7) KRTAP type 19 family Q3SYF9
Keratin-associated protein 3-3 (KRTAP3-3) KRTAP type 3 family Q9BYR6
Keratin-associated protein 6-1 (KRTAP6-1) KRTAP type 6 family Q3LI64
Keratin-associated protein 6-2 (KRTAP6-2) KRTAP type 6 family Q3LI66
Keratin-associated protein 7-1 (KRTAP7-1) KRTAP type 7 family Q8IUC3
Keratin-associated protein 8-1 (KRTAP8-1) KRTAP type 8 family Q8IUC2
Odontogenic ameloblast-associated protein (ODAM) ODAM family A1E959
Keratin-associated protein 15-1 (KRTAP15-1) PMG family Q3LI76
Keratin-associated protein 26-1 (KRTAP26-1) PMG family Q6PEX3
Paraneoplastic antigen Ma1 (PNMA1) PNMA family Q8ND90
Proline-rich protein 20D (PRR20D) PRR20 family P86480
Migration and invasion enhancer 1 (MIEN1) SelWTH family Q9BRT3
tRNA-splicing endonuclease subunit Sen15 (TSEN15) SEN15 family Q8WW01
SOSS complex subunit C (INIP) SOSS-C family Q9NRY2
Tektin-3 (TEKT3) Tektin family Q9BXF9
Tektin-4 (TEKT4) Tektin family Q8WW24
Protein VAC14 homolog (VAC14) VAC14 family Q08AM6
BAG family molecular chaperone regulator 4 (BAG4) . O95429
Bromo adjacent homology domain-containing 1 protein (BAHD1) . Q8TBE0
BTB/POZ domain-containing protein KCTD9 (KCTD9) . Q7L273
Coiled-coil domain-containing protein 120 (CCDC120) . Q96HB5
DAZ-associated protein 2 (DAZAP2) . Q15038
Four and a half LIM domains protein 3 (FHL3) . Q13643
Keratinocyte differentiation factor 1 (KDF1) . Q8NAX2
Leukocyte receptor cluster member 8 (LENG8) . Q96PV6
LIM and senescent cell antigen-like-containing domain protein 4 (LIMS4) . P0CW20
Melanoma-associated antigen D1 (MAGED1) . Q9Y5V3
MORN repeat-containing protein 3 (MORN3) . Q6PF18
RING finger protein 44 (RNF44) . Q7L0R7
RNA-binding protein with multiple splicing 2 (RBPMS2) . Q6ZRY4
TNF receptor-associated factor 1 (TRAF1) . Q13077
Uncharacterized protein C14orf119 (C14orf119) . Q9NWQ9
Zinc finger CCCH domain-containing protein 10 (ZC3H10) . Q96K80

References

1 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402