Details of the Target

General Information of Target

Target ID LDTP04922
Target Name Large ribosomal subunit protein eL27 (RPL27)
Gene Name RPL27
Gene ID 6155
Synonyms
Large ribosomal subunit protein eL27; 60S ribosomal protein L27
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKK
KIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEE
RYKTGKNKWFFQKLRF
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eL27 family
Subcellular location
Cytoplasm, cytosol
Function Component of the large ribosomal subunit. Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs.
Uniprot ID
P61353
Ensemble ID
ENST00000253788.12
HGNC ID
HGNC:10328

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-EBA
 Probe Info  		2.64  LDD0215  [1]
C-Sul
 Probe Info  		6.30  LDD0066  [2]
TH211
 Probe Info  		Y75(20.00); Y38(16.78); Y77(14.23)  LDD0257  [3]
TH214
 Probe Info  		Y75(5.24)  LDD0258  [3]
TH216
 Probe Info  		Y77(20.00); Y75(10.12); Y85(10.09)  LDD0259  [3]
ONAyne
 Probe Info  		K117(0.00); K93(0.00); K98(0.00); K52(0.00)  LDD0273  [4]
OPA-S-S-alkyne
 Probe Info  		K98(1.21)  LDD3494  [5]
ATP probe
 Probe Info  		K52(0.00); K128(0.00); K73(0.00); K98(0.00)  LDD0199  [6]
m-APA
 Probe Info  		N.A.  LDD2231  [7]
1d-yne
 Probe Info  		N.A.  LDD0358  [8]
ATP probe
 Probe Info  		K52(0.00); K133(0.00); K59(0.00)  LDD0035  [9]
NHS
 Probe Info  		K93(0.00); K27(0.00)  LDD0010  [10]
SF
 Probe Info  		Y38(0.00); Y85(0.00)  LDD0028  [11]
STPyne
 Probe Info  		K93(0.00); K27(0.00)  LDD0009  [10]
Ox-W18
 Probe Info  		N.A.  LDD2175  [12]
1c-yne
 Probe Info  		N.A.  LDD0228  [8]
Acrolein
 Probe Info  		N.A.  LDD0217  [13]
MPP-AC
 Probe Info  		N.A.  LDD0428  [14]
HHS-482
 Probe Info  		Y85(1.16)  LDD2239  [15]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [16]
STS-2
 Probe Info  		N.A.  LDD0138  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H40(0.00); H79(0.00)  LDD0222  [13]
 LDCM0107  IAA HeLa H79(0.00); H40(0.00)  LDD0221  [13]
 LDCM0109  NEM HeLa H79(0.00); H40(0.00)  LDD0223  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ribosomal RNA processing protein 1 homolog B (RRP1B) RRP1 family Q14684

References

1 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
9 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
15 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
16 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.