General Information of Target

Target ID LDTP04536
Target Name Ephrin-A5 (EFNA5)
Gene Name EFNA5
Gene ID 1946
Synonyms
EPLG7; LERK7; Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLHVEMLTLVFLVLWMCVFSQDPGSKAVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDV
FCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQL
FTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDK
VENSLEPADDTVHESAEPSRGENAAQTPRIPSRLLAILLFLLAMLLTL
Target Bioclass
Other
Family
Ephrin family
Subcellular location
Cell membrane
Function
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Uniprot ID
P52803
Ensemble ID
ENST00000333274.11
HGNC ID
HGNC:3225

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info 
K166(0.58); K180(6.21)  LDD3494  [1]
DBIA
 Probe Info 
C164(1.97)  LDD3360  [2]
IA-alkyne
 Probe Info 
N.A.  LDD0165  [3]
NAIA_5
 Probe Info 
N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C164(1.08)  LDD1507  [5]
 LDCM0215  AC10 HEK-293T C164(1.11); C53(0.89)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C53(1.46)  LDD1509  [5]
 LDCM0237  AC12 HEK-293T C53(0.95)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C164(1.25)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C164(1.00)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C164(1.15)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C164(1.13); C53(1.16)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C53(1.15)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C164(1.03); C53(1.03)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C53(1.28)  LDD1519  [5]
 LDCM0282  AC22 HEK-293T C164(1.03)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C164(1.01)  LDD1522  [5]
 LDCM0285  AC25 HEK-293T C164(1.07)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C164(1.11); C53(0.90)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C53(1.14)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C53(1.05)  LDD1527  [5]
 LDCM0290  AC3 HEK-293T C53(1.08)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C164(1.13)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C164(0.99)  LDD1531  [5]
 LDCM0294  AC33 HEK-293T C164(1.07)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C164(1.05); C53(1.15)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C53(1.15)  LDD1535  [5]
 LDCM0297  AC36 HEK-293T C53(1.28)  LDD1536  [5]
 LDCM0299  AC38 HEK-293T C164(1.29)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C164(1.02)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C53(0.99)  LDD1540  [5]
 LDCM0303  AC41 HEK-293T C164(1.10)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C164(1.08); C53(1.08)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C53(1.16)  LDD1544  [5]
 LDCM0306  AC44 HEK-293T C53(1.24)  LDD1545  [5]
 LDCM0308  AC46 HEK-293T C164(1.23)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C164(1.06)  LDD1548  [5]
 LDCM0311  AC49 HEK-293T C164(1.08)  LDD1550  [5]
 LDCM0313  AC50 HEK-293T C164(0.97); C53(0.87)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C53(0.93)  LDD1553  [5]
 LDCM0315  AC52 HEK-293T C53(1.24)  LDD1554  [5]
 LDCM0317  AC54 HEK-293T C164(1.13)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C164(1.07)  LDD1557  [5]
 LDCM0320  AC57 HEK-293T C164(1.13)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C164(1.01); C53(1.27)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C53(1.26)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C164(1.26)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C53(1.08)  LDD1563  [5]
 LDCM0326  AC62 HEK-293T C164(1.12)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C164(1.03)  LDD1566  [5]
 LDCM0334  AC7 HEK-293T C164(1.03)  LDD1568  [5]
 LDCM0356  AKOS034007680 HEK-293T C164(1.15)  LDD1570  [5]
 LDCM0368  CL10 HEK-293T C164(1.40)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C164(1.11); C53(0.87)  LDD1573  [5]
 LDCM0371  CL102 HEK-293T C164(1.18); C53(0.96)  LDD1575  [5]
 LDCM0372  CL103 HEK-293T C164(1.11); C53(0.94)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C164(0.99); C53(0.84)  LDD1577  [5]
 LDCM0375  CL106 HEK-293T C164(1.06); C53(0.96)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C164(0.96); C53(1.21)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C164(1.03); C53(0.94)  LDD1581  [5]
 LDCM0379  CL11 HEK-293T C164(1.09)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C164(1.09); C53(0.98)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C164(1.09); C53(0.75)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C164(1.14); C53(0.76)  LDD1586  [5]
 LDCM0384  CL114 HEK-293T C164(1.22); C53(1.35)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C164(1.10); C53(1.03)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C164(1.05); C53(1.32)  LDD1590  [5]
 LDCM0388  CL118 HEK-293T C164(1.00); C53(1.03)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C164(1.13); C53(0.98)  LDD1593  [5]
 LDCM0391  CL120 HEK-293T C164(0.99); C53(0.84)  LDD1595  [5]
 LDCM0393  CL122 HEK-293T C164(0.99); C53(1.04)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C164(1.05); C53(0.84)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C164(1.06); C53(1.07)  LDD1599  [5]
 LDCM0397  CL126 HEK-293T C164(1.27); C53(1.13)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C164(1.12); C53(0.94)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C164(1.10); C53(1.08)  LDD1603  [5]
 LDCM0401  CL14 HEK-293T C164(1.14); C53(1.44)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C164(1.05); C53(0.75)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C164(1.06); C53(1.08)  LDD1607  [5]
 LDCM0404  CL17 HEK-293T C164(1.01)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C164(1.04); C53(1.10)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C53(1.12)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C164(1.10); C53(1.17)  LDD1611  [5]
 LDCM0408  CL20 HEK-293T C53(1.17)  LDD1612  [5]
 LDCM0410  CL22 HEK-293T C164(1.09)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C164(1.07)  LDD1615  [5]
 LDCM0414  CL26 HEK-293T C164(0.94); C53(1.15)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C164(0.98); C53(0.80)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C164(0.96); C53(1.13)  LDD1620  [5]
 LDCM0417  CL29 HEK-293T C164(1.01)  LDD1621  [5]
 LDCM0418  CL3 HEK-293T C164(0.94); C53(1.01)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C164(1.05); C53(1.36)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C53(1.20)  LDD1624  [5]
 LDCM0421  CL32 HEK-293T C53(1.04)  LDD1625  [5]
 LDCM0423  CL34 HEK-293T C164(1.13)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C164(1.09)  LDD1628  [5]
 LDCM0428  CL39 HEK-293T C164(1.06); C53(0.93)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C164(1.01); C53(0.92)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C164(1.01); C53(0.91)  LDD1634  [5]
 LDCM0431  CL41 HEK-293T C164(0.99)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C164(1.00); C53(1.40)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C53(1.11)  LDD1637  [5]
 LDCM0434  CL44 HEK-293T C53(1.06)  LDD1638  [5]
 LDCM0436  CL46 HEK-293T C164(1.17)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C164(1.08)  LDD1641  [5]
 LDCM0440  CL5 HEK-293T C164(0.95)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C164(0.97); C53(1.14)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C164(0.95); C53(0.94)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C164(1.08)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C164(0.97); C53(1.12)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C53(1.33)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C53(1.03)  LDD1650  [5]
 LDCM0449  CL58 HEK-293T C164(1.29)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C164(1.03)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C164(1.06); C53(1.35)  LDD1654  [5]
 LDCM0454  CL62 HEK-293T C164(1.04); C53(0.98)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C164(1.04); C53(0.92)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C164(0.89); C53(0.84)  LDD1659  [5]
 LDCM0457  CL65 HEK-293T C164(1.01)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C164(1.05); C53(1.21)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C53(1.17)  LDD1662  [5]
 LDCM0460  CL68 HEK-293T C53(1.16)  LDD1663  [5]
 LDCM0462  CL7 HEK-293T C53(1.41)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C164(1.08)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C164(1.03)  LDD1667  [5]
 LDCM0467  CL74 HEK-293T C164(1.10); C53(1.16)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C164(1.00); C53(0.95)  LDD1672  [5]
 LDCM0470  CL77 HEK-293T C164(1.10)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C164(1.00); C53(0.94)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C53(1.20)  LDD1675  [5]
 LDCM0473  CL8 HEK-293T C53(1.23)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C53(1.27)  LDD1677  [5]
 LDCM0476  CL82 HEK-293T C164(1.25)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C164(1.13)  LDD1680  [5]
 LDCM0480  CL86 HEK-293T C164(1.04); C53(1.23)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C164(1.06); C53(0.91)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C164(0.98); C53(1.02)  LDD1685  [5]
 LDCM0483  CL89 HEK-293T C164(1.14)  LDD1686  [5]
 LDCM0485  CL90 HEK-293T C164(1.15); C53(0.87)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C53(1.32)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C53(1.27)  LDD1690  [5]
 LDCM0489  CL94 HEK-293T C164(1.27)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C164(1.21)  LDD1693  [5]
 LDCM0493  CL98 HEK-293T C164(1.07); C53(1.03)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C164(1.13); C53(0.87)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C164(1.10); C53(1.11)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C164(1.07); C53(0.88)  LDD1671  [5]
 LDCM0427  Fragment51 HEK-293T C164(1.08); C53(1.08)  LDD1631  [5]
 LDCM0022  KB02 ICC106 C164(1.44)  LDD2380  [2]
 LDCM0023  KB03 ICC10 C164(1.72)  LDD2796  [2]
 LDCM0024  KB05 NCI-H3122 C164(1.97)  LDD3360  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sphingosine-1-phosphate lyase 1 (SGPL1) Group II decarboxylase family O95470
3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) HIBADH-related family P31937
Ephrin type-A receptor 2 (EPHA2) Tyr protein kinase family P29317
Ephrin type-A receptor 4 (EPHA4) Tyr protein kinase family P54764
Transmembrane ascorbate-dependent reductase CYB561 (CYB561) . P49447
Transporter and channel
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ammonium transporter Rh type C (RHCG) Ammonium transporter family Q9UBD6
Bestrophin-2 (BEST2) Anion channel-forming bestrophin family Q8NFU1
Ileal sodium/bile acid cotransporter (SLC10A2) Bile acid:sodium symporter (BASS) family Q12908
Claudin-5 (CLDN5) Claudin family O00501
Gap junction beta-1 protein (GJB1) Connexin family P08034
Transmembrane 4 L6 family member 18 (TM4SF18) L6 tetraspanin family Q96CE8
Aquaporin-2 (AQP2) MIP/aquaporin (TC 1.A.8) family P41181
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Membrane-spanning 4-domains subfamily A member 3 (MS4A3) MS4A family Q96HJ5
Solute carrier family 13 member 4 (SLC13A4) SLC13A/DASS transporter family Q9UKG4
Leukocyte surface antigen CD53 (CD53) Tetraspanin (TM4SF) family P19397
Translocator protein 2 (TSPO2) TspO/BZRP family Q5TGU0
GPCR
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Free fatty acid receptor 3 (FFAR3) G-protein coupled receptor 1 family O14843
G-protein coupled receptor 151 (GPR151) G-protein coupled receptor 1 family Q8TDV0
G-protein coupled receptor 161 (GPR161) G-protein coupled receptor 1 family Q8N6U8
G-protein coupled receptor 42 (GPR42) G-protein coupled receptor 1 family O15529
Probable G-protein coupled receptor 152 (GPR152) G-protein coupled receptor 1 family Q8TDT2
Thyrotropin-releasing hormone receptor (TRHR) G-protein coupled receptor 1 family P34981
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Atypical chemokine receptor 2 (ACKR2) G-protein coupled receptor 1 family O00590
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein YIPF6 (YIPF6) YIP1 family Q96EC8
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein (HERPUD2) . Q9BSE4
Protein KASH5 (KASH5) . Q8N6L0
Serine-rich single-pass membrane protein 1 (SSMEM1) . Q8WWF3

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402