Details of the Target

General Information of Target

Target ID LDTP04526
Target Name Zinc finger protein 143 (ZNF143)
Gene Name ZNF143
Gene ID 7702
Synonyms
SBF; STAF; Zinc finger protein 143; SPH-binding factor; Selenocysteine tRNA gene transcription-activating factor; hStaf
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLLAQINRDSQGMTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGST
AYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIH
HTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDT
ISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCE
YDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCS
EDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCT
EPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCN
HCGKTYKQISTLAMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDD
VVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDA
VISSAGTHSVAMVTAEGTEGEQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLV
ATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD
Target Bioclass
Transcription factor
Family
GLI C2H2-type zinc-finger protein family
Subcellular location
Nucleus
Function
Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.
Uniprot ID
P52747
Ensemble ID
ENST00000396597.7
HGNC ID
HGNC:12928

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.L72F .
EFO27 SNV: p.E43K .
HT115 SNV: p.I160M .
JURKAT SNV: p.D57N .
LS180 SNV: p.H381Y .
MEWO SNV: p.P17S; p.S196N .
MOLT4 SNV: p.G605D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K285(10.00)  LDD0277  [1]
DBIA
 Probe Info  		C329(50.49)  LDD0209  [2]
Phosphinate-6
 Probe Info  		C299(0.00); C269(0.00); C329(0.00); C304(0.00)  LDD0018  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C329(1.40)  LDD0531  [4]
 LDCM0276  AC17 HCT 116 C329(0.85)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C329(0.70)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C329(0.81)  LDD0595  [4]
 LDCM0279  AC2 HCT 116 C329(1.44)  LDD0596  [4]
 LDCM0280  AC20 HCT 116 C329(0.53)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C329(0.61)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C329(0.67)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C329(0.58)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C329(0.58)  LDD0601  [4]
 LDCM0290  AC3 HCT 116 C329(1.61)  LDD0607  [4]
 LDCM0301  AC4 HCT 116 C329(1.86)  LDD0618  [4]
 LDCM0312  AC5 HCT 116 C329(1.32)  LDD0629  [4]
 LDCM0020  ARS-1620 HCC44 C239(2.14); C244(2.14); C269(2.27); C274(2.27)  LDD2171  [4]
 LDCM0367  CL1 HCT 116 C329(1.01)  LDD0684  [4]
 LDCM0368  CL10 HCT 116 C329(0.66)  LDD0685  [4]
 LDCM0369  CL100 HCT 116 C329(1.58)  LDD0686  [4]
 LDCM0374  CL105 HCT 116 C329(0.71)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C329(0.68)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C329(0.82)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C329(0.76)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C329(0.85)  LDD0695  [4]
 LDCM0379  CL11 HCT 116 C329(0.63)  LDD0696  [4]
 LDCM0380  CL110 HCT 116 C329(0.65)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C329(0.72)  LDD0698  [4]
 LDCM0390  CL12 HCT 116 C329(0.77)  LDD0707  [4]
 LDCM0400  CL13 HCT 116 C329(0.73)  LDD0717  [4]
 LDCM0401  CL14 HCT 116 C329(0.78)  LDD0718  [4]
 LDCM0402  CL15 HCT 116 C329(0.61)  LDD0719  [4]
 LDCM0407  CL2 HCT 116 C329(0.72)  LDD0724  [4]
 LDCM0418  CL3 HCT 116 C329(0.72)  LDD0735  [4]
 LDCM0429  CL4 HCT 116 C329(0.75)  LDD0746  [4]
 LDCM0436  CL46 HCT 116 C329(0.97)  LDD0753  [4]
 LDCM0437  CL47 HCT 116 C329(1.00)  LDD0754  [4]
 LDCM0438  CL48 HCT 116 C329(0.93)  LDD0755  [4]
 LDCM0439  CL49 HCT 116 C329(0.97)  LDD0756  [4]
 LDCM0440  CL5 HCT 116 C329(0.77)  LDD0757  [4]
 LDCM0441  CL50 HCT 116 C329(1.09)  LDD0758  [4]
 LDCM0442  CL51 HCT 116 C329(1.02)  LDD0759  [4]
 LDCM0443  CL52 HCT 116 C329(1.14)  LDD0760  [4]
 LDCM0444  CL53 HCT 116 C329(0.83)  LDD0761  [4]
 LDCM0445  CL54 HCT 116 C329(0.85)  LDD0762  [4]
 LDCM0446  CL55 HCT 116 C329(0.99)  LDD0763  [4]
 LDCM0447  CL56 HCT 116 C329(1.05)  LDD0764  [4]
 LDCM0448  CL57 HCT 116 C329(0.93)  LDD0765  [4]
 LDCM0449  CL58 HCT 116 C329(1.21)  LDD0766  [4]
 LDCM0450  CL59 HCT 116 C329(0.99)  LDD0767  [4]
 LDCM0451  CL6 HCT 116 C329(0.79)  LDD0768  [4]
 LDCM0452  CL60 HCT 116 C329(1.12)  LDD0769  [4]
 LDCM0453  CL61 HCT 116 C329(1.07)  LDD0770  [4]
 LDCM0454  CL62 HCT 116 C329(1.35)  LDD0771  [4]
 LDCM0455  CL63 HCT 116 C329(1.18)  LDD0772  [4]
 LDCM0456  CL64 HCT 116 C329(1.28)  LDD0773  [4]
 LDCM0457  CL65 HCT 116 C329(1.25)  LDD0774  [4]
 LDCM0458  CL66 HCT 116 C329(1.29)  LDD0775  [4]
 LDCM0459  CL67 HCT 116 C329(1.24)  LDD0776  [4]
 LDCM0460  CL68 HCT 116 C329(1.08)  LDD0777  [4]
 LDCM0461  CL69 HCT 116 C329(1.06)  LDD0778  [4]
 LDCM0462  CL7 HCT 116 C329(0.91)  LDD0779  [4]
 LDCM0463  CL70 HCT 116 C329(0.96)  LDD0780  [4]
 LDCM0464  CL71 HCT 116 C329(1.13)  LDD0781  [4]
 LDCM0465  CL72 HCT 116 C329(1.26)  LDD0782  [4]
 LDCM0466  CL73 HCT 116 C329(1.19)  LDD0783  [4]
 LDCM0467  CL74 HCT 116 C329(1.39)  LDD0784  [4]
 LDCM0469  CL76 HCT 116 C329(0.74)  LDD0786  [4]
 LDCM0470  CL77 HCT 116 C329(0.69)  LDD0787  [4]
 LDCM0471  CL78 HCT 116 C329(0.96)  LDD0788  [4]
 LDCM0472  CL79 HCT 116 C329(0.92)  LDD0789  [4]
 LDCM0473  CL8 HCT 116 C329(0.62)  LDD0790  [4]
 LDCM0474  CL80 HCT 116 C329(0.77)  LDD0791  [4]
 LDCM0475  CL81 HCT 116 C329(1.15)  LDD0792  [4]
 LDCM0476  CL82 HCT 116 C329(0.90)  LDD0793  [4]
 LDCM0477  CL83 HCT 116 C329(0.52)  LDD0794  [4]
 LDCM0478  CL84 HCT 116 C329(0.81)  LDD0795  [4]
 LDCM0479  CL85 HCT 116 C329(0.76)  LDD0796  [4]
 LDCM0480  CL86 HCT 116 C329(0.67)  LDD0797  [4]
 LDCM0481  CL87 HCT 116 C329(0.68)  LDD0798  [4]
 LDCM0482  CL88 HCT 116 C329(0.77)  LDD0799  [4]
 LDCM0483  CL89 HCT 116 C329(0.84)  LDD0800  [4]
 LDCM0484  CL9 HCT 116 C329(0.76)  LDD0801  [4]
 LDCM0485  CL90 HCT 116 C329(0.59)  LDD0802  [4]
 LDCM0486  CL91 HCT 116 C329(1.31)  LDD0803  [4]
 LDCM0487  CL92 HCT 116 C329(1.21)  LDD0804  [4]
 LDCM0488  CL93 HCT 116 C329(0.94)  LDD0805  [4]
 LDCM0489  CL94 HCT 116 C329(1.13)  LDD0806  [4]
 LDCM0490  CL95 HCT 116 C329(1.14)  LDD0807  [4]
 LDCM0491  CL96 HCT 116 C329(1.61)  LDD0808  [4]
 LDCM0492  CL97 HCT 116 C329(1.53)  LDD0809  [4]
 LDCM0493  CL98 HCT 116 C329(1.30)  LDD0810  [4]
 LDCM0494  CL99 HCT 116 C329(1.24)  LDD0811  [4]
 LDCM0468  Fragment33 HCT 116 C329(1.03)  LDD0785  [4]
 LDCM0022  KB02 HEK-293T C274(1.24)  LDD1492  [5]
 LDCM0023  KB03 Jurkat C329(50.49)  LDD0209  [2]
 LDCM0024  KB05 HEK-293T C274(0.92)  LDD1502  [5]
 LDCM0021  THZ1 HCT 116 C239(2.14); C244(2.14); C269(2.27); C274(2.27)  LDD2173  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Exosome complex component CSL4 (EXOSC1) CSL4 family Q9Y3B2
Uridine diphosphate glucose pyrophosphatase NUDT22 (NUDT22) Nudix hydrolase family Q9BRQ3
Sulfite oxidase, mitochondrial (SUOX) . P51687
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 438 (ZNF438) Krueppel C2H2-type zinc-finger protein family Q7Z4V0
Zinc finger protein 512B (ZNF512B) Krueppel C2H2-type zinc-finger protein family Q96KM6
Pogo transposable element with ZNF domain (POGZ) . Q7Z3K3
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Splicing factor YJU2 (YJU2) CWC16 family Q9BW85
ELL-associated factor 1 (EAF1) EAF family Q96JC9
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
Protein lin-54 homolog (LIN54) Lin-54 family Q6MZP7
Sperm protein associated with the nucleus on the X chromosome N3 (SPANXN3) SPAN-X family Q5MJ09
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
Four and a half LIM domains protein 2 (FHL2) . Q14192
IQ motif and ubiquitin-like domain-containing protein (IQUB) . Q8NA54
Rhombotin-1 (LMO1) . P25800
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (TAB2) . Q9NYJ8

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402