Details of the Target

General Information of Target

Target ID LDTP04475
Target Name cAMP-dependent protein kinase catalytic subunit PRKX (PRKX)
Gene Name PRKX
Gene ID 5613
Synonyms
PKX1; cAMP-dependent protein kinase catalytic subunit PRKX; PrKX; Protein kinase X; Protein kinase X-linked; Serine/threonine-protein kinase PRKX; EC 2.7.11.1; Protein kinase PKX1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEAPGLAQAAAAESDSRKVAEETPDGAPALCPSPEALSPEPPVYSLQDFDTLATVGTGTF
GRVHLVKEKTAKHFFALKVMSIPDVIRLKQEQHVHNEKSVLKEVSHPFLIRLFWTWHDER
FLYMLMEYVPGGELFSYLRNRGRFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILL
DRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSG
FPPFFDDNPFGIYQKILAGKIDFPRHLDFHVKDLIKKLLVVDRTRRLGNMKNGANDVKHH
RWFRSVDWEAVPQRKLKPPIVPKIAGDGDTSNFETYPENDWDTAAPVPQKDLEIFKNF
Target Bioclass
Enzyme
Family
Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
Subcellular location
Cytoplasm
Function
Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
Uniprot ID
P51817
Ensemble ID
ENST00000262848.6
HGNC ID
HGNC:9441
ChEMBL ID
CHEMBL5818

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C205(0.95)  LDD0531  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C205(0.95)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C205(0.96)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C205(0.99)  LDD0543  [1]
 LDCM0237  AC12 HCT 116 C205(0.85)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C205(0.84)  LDD0576  [1]
 LDCM0263  AC143 HCT 116 C205(0.92)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C205(1.04)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C205(1.30)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C205(1.34)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C205(1.11)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C205(3.33)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C205(1.83)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C205(1.17)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C205(1.19)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C205(1.30)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C205(1.46)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C205(1.77)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C205(1.13)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C205(1.23)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C205(1.22)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C205(1.29)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C205(0.98)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C205(1.01)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C205(0.99)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C205(1.22)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C205(1.01)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C205(0.92)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C205(0.96)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C205(0.96)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C205(0.95)  LDD0601  [1]
 LDCM0290  AC3 HCT 116 C205(1.19)  LDD0607  [1]
 LDCM0291  AC30 HEK-293T C205(1.01)  LDD1530  [2]
 LDCM0299  AC38 HEK-293T C205(0.91)  LDD1538  [2]
 LDCM0301  AC4 HCT 116 C205(1.38)  LDD0618  [1]
 LDCM0308  AC46 HCT 116 C205(0.96)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C205(0.83)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C205(0.61)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C205(1.05)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C205(1.17)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C205(1.00)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C205(0.88)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C205(0.61)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C205(0.82)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C205(0.80)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C205(0.74)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C205(0.59)  LDD0636  [1]
 LDCM0323  AC6 HCT 116 C205(0.94)  LDD0640  [1]
 LDCM0326  AC62 HEK-293T C205(0.93)  LDD1565  [2]
 LDCM0334  AC7 HCT 116 C205(1.30)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C205(1.00)  LDD0662  [1]
 LDCM0349  AC83 HCT 116 C205(0.76)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C205(0.71)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C205(0.73)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C205(0.63)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C205(1.00)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C205(0.63)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C205(0.78)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C205(0.87)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C205(0.64)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C205(0.65)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C205(0.66)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C205(0.67)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C205(0.73)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C205(0.71)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C205(0.77)  LDD0681  [1]
 LDCM0248  AKOS034007472 HCT 116 C205(0.84)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C205(0.94)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C205(0.87)  LDD0592  [1]
 LDCM0368  CL10 HEK-293T C205(1.94)  LDD1572  [2]
 LDCM0369  CL100 HCT 116 C205(1.17)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C205(0.92)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C205(0.84)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C205(0.81)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C205(0.72)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C205(0.88)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C205(0.96)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C205(0.89)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C205(0.91)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C205(0.96)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C205(0.98)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C205(0.98)  LDD0698  [1]
 LDCM0392  CL121 HCT 116 C205(0.77)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C205(0.74)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C205(0.46)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C205(0.57)  LDD0712  [1]
 LDCM0410  CL22 HEK-293T C205(1.14)  LDD1614  [2]
 LDCM0423  CL34 HEK-293T C205(1.28)  LDD1627  [2]
 LDCM0436  CL46 HCT 116 C205(0.97)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C205(1.13)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C205(1.05)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C205(0.88)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C205(1.15)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C205(1.07)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C205(1.07)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C205(0.83)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C205(0.81)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C205(1.03)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C205(0.96)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C205(1.01)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C205(1.07)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C205(0.90)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C205(1.22)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C205(0.91)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C205(0.95)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C205(0.83)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C205(0.83)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C205(1.16)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C205(0.92)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C205(0.78)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C205(0.82)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C205(0.68)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C205(0.87)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C205(0.79)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C205(0.83)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C205(0.75)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C205(0.73)  LDD0784  [1]
 LDCM0476  CL82 HEK-293T C205(1.03)  LDD1679  [2]
 LDCM0486  CL91 HCT 116 C205(1.03)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C205(0.83)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C205(0.88)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C205(1.07)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C205(1.01)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C205(1.01)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C205(1.22)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C205(0.87)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C205(1.04)  LDD0811  [1]
 LDCM0468  Fragment33 HCT 116 C205(0.84)  LDD0785  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Heat shock protein HSP 90-beta (HSP90AB1) Heat shock protein 90 family P08238
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 6 (SMAD6) Dwarfin/SMAD family O43541
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) CAMP-dependent kinase regulatory chain family P10644
G-patch domain and KOW motifs-containing protein (GPKOW) MOS2 family Q92917

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402