Details of the Target

General Information of Target

Target ID LDTP03716
Target Name Nitric oxide synthase, inducible (NOS2)
Gene Name NOS2
Gene ID 4843
Synonyms
NOS2A; Nitric oxide synthase, inducible; EC 1.14.13.39; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPL
VETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIM
TPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQ
LTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNI
RSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYG
RFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVG
GLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINI
AVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEM
LNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVT
ILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPG
NGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGD
ELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDL
SKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQ
PALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQ
LLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQL
PILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCF
VRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPD
EDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLY
VCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDR
VAVQPSSLEMSAL
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
NOS family
Subcellular location
Cytoplasm, cytosol
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
TTD ID
T02703
Uniprot ID
P35228
DrugMap ID
TTF10I9
Ensemble ID
ENST00000313735.11
HGNC ID
HGNC:7873
ChEMBL ID
CHEMBL4481

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.Y299C; p.Y621H .
CAL51 SNV: p.P467H .
COLO678 SNV: p.K1053N .
DU145 SNV: p.G375V .
FTC133 SNV: p.V686A .
HCT15 SNV: p.P514S .
HGC27 SNV: p.W461C .
HT115 SNV: p.F1128C .
HUPT4 SNV: p.D137E .
MDST8 SNV: p.E320K .
MKN45 SNV: p.P970L .
NCIH1993 Deletion: p.A459QfsTer23 .
NCIH446 SNV: p.Q45Ter .
PC9 SNV: p.P706T .
SNU5 SNV: p.K1003N .
TOV21G SNV: p.W672L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C526(0.96)  LDD1507  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C526(0.96)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C526(0.94)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C526(0.93)  LDD1509  [1]
 LDCM0259  AC14 HEK-293T C526(0.92)  LDD1512  [1]
 LDCM0270  AC15 HEK-293T C526(1.01); C579(0.99)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C526(1.07)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C526(0.99)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C526(1.04)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C526(0.91)  LDD1518  [1]
 LDCM0282  AC22 HEK-293T C526(1.00)  LDD1521  [1]
 LDCM0283  AC23 HEK-293T C526(0.97); C579(1.11)  LDD1522  [1]
 LDCM0284  AC24 HEK-293T C526(0.98)  LDD1523  [1]
 LDCM0285  AC25 HEK-293T C526(1.07)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C526(0.98)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C526(1.04)  LDD1526  [1]
 LDCM0290  AC3 HEK-293T C526(0.99)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C526(0.96)  LDD1530  [1]
 LDCM0292  AC31 HEK-293T C526(0.99); C579(1.02)  LDD1531  [1]
 LDCM0293  AC32 HEK-293T C526(1.06)  LDD1532  [1]
 LDCM0294  AC33 HEK-293T C526(0.95)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C526(0.94)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C526(1.00)  LDD1535  [1]
 LDCM0299  AC38 HEK-293T C526(0.90)  LDD1538  [1]
 LDCM0300  AC39 HEK-293T C526(1.08); C579(1.00)  LDD1539  [1]
 LDCM0302  AC40 HEK-293T C526(1.07)  LDD1541  [1]
 LDCM0303  AC41 HEK-293T C526(1.12)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C526(1.03)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C526(0.99)  LDD1544  [1]
 LDCM0308  AC46 HEK-293T C526(0.97)  LDD1547  [1]
 LDCM0309  AC47 HEK-293T C526(1.09); C579(1.11)  LDD1548  [1]
 LDCM0310  AC48 HEK-293T C526(0.98)  LDD1549  [1]
 LDCM0311  AC49 HEK-293T C526(0.98)  LDD1550  [1]
 LDCM0313  AC50 HEK-293T C526(0.99)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C526(1.00)  LDD1553  [1]
 LDCM0317  AC54 HEK-293T C526(1.02)  LDD1556  [1]
 LDCM0318  AC55 HEK-293T C526(1.03); C579(1.06)  LDD1557  [1]
 LDCM0319  AC56 HEK-293T C526(1.07)  LDD1558  [1]
 LDCM0320  AC57 HEK-293T C526(1.02)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C526(0.97)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C526(0.98)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C526(0.96)  LDD1562  [1]
 LDCM0326  AC62 HEK-293T C526(0.95)  LDD1565  [1]
 LDCM0327  AC63 HEK-293T C526(1.05); C579(0.87)  LDD1566  [1]
 LDCM0328  AC64 HEK-293T C526(0.98)  LDD1567  [1]
 LDCM0334  AC7 HEK-293T C526(0.91); C579(1.00)  LDD1568  [1]
 LDCM0345  AC8 HEK-293T C526(1.00)  LDD1569  [1]
 LDCM0356  AKOS034007680 HEK-293T C526(1.03)  LDD1570  [1]
 LDCM0275  AKOS034007705 HEK-293T C526(1.04)  LDD1514  [1]
 LDCM0368  CL10 HEK-293T C526(1.00)  LDD1572  [1]
 LDCM0379  CL11 HEK-293T C526(1.07); C579(1.22)  LDD1583  [1]
 LDCM0390  CL12 HEK-293T C526(1.08)  LDD1594  [1]
 LDCM0404  CL17 HEK-293T C526(1.02)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C526(1.05)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C526(0.99)  LDD1610  [1]
 LDCM0410  CL22 HEK-293T C526(1.04)  LDD1614  [1]
 LDCM0411  CL23 HEK-293T C526(1.06); C579(1.19)  LDD1615  [1]
 LDCM0412  CL24 HEK-293T C526(1.12)  LDD1616  [1]
 LDCM0417  CL29 HEK-293T C526(1.07)  LDD1621  [1]
 LDCM0419  CL30 HEK-293T C526(1.04)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C526(1.08)  LDD1624  [1]
 LDCM0423  CL34 HEK-293T C526(1.06)  LDD1627  [1]
 LDCM0424  CL35 HEK-293T C526(1.02); C579(1.41)  LDD1628  [1]
 LDCM0425  CL36 HEK-293T C526(1.16)  LDD1629  [1]
 LDCM0431  CL41 HEK-293T C526(1.09)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C526(1.03)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C526(0.97)  LDD1637  [1]
 LDCM0436  CL46 HEK-293T C526(1.07)  LDD1640  [1]
 LDCM0437  CL47 HEK-293T C526(0.91); C579(1.08)  LDD1641  [1]
 LDCM0438  CL48 HEK-293T C526(1.17)  LDD1642  [1]
 LDCM0440  CL5 HEK-293T C526(0.99)  LDD1644  [1]
 LDCM0444  CL53 HEK-293T C526(1.06)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C526(1.12)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C526(0.99)  LDD1649  [1]
 LDCM0449  CL58 HEK-293T C526(1.04)  LDD1652  [1]
 LDCM0450  CL59 HEK-293T C526(1.10); C579(1.12)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C526(1.07)  LDD1654  [1]
 LDCM0452  CL60 HEK-293T C526(1.22)  LDD1655  [1]
 LDCM0457  CL65 HEK-293T C526(0.97)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C526(1.06)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C526(1.01)  LDD1662  [1]
 LDCM0462  CL7 HEK-293T C526(0.99)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C526(1.15)  LDD1666  [1]
 LDCM0464  CL71 HEK-293T C526(1.10); C579(1.37)  LDD1667  [1]
 LDCM0465  CL72 HEK-293T C526(1.16)  LDD1668  [1]
 LDCM0470  CL77 HEK-293T C526(1.23)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C526(1.02)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C526(1.01)  LDD1675  [1]
 LDCM0476  CL82 HEK-293T C526(1.06)  LDD1679  [1]
 LDCM0477  CL83 HEK-293T C526(1.06); C579(1.11)  LDD1680  [1]
 LDCM0478  CL84 HEK-293T C526(1.13)  LDD1681  [1]
 LDCM0483  CL89 HEK-293T C526(1.06)  LDD1686  [1]
 LDCM0485  CL90 HEK-293T C526(1.14)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C526(1.04)  LDD1689  [1]
 LDCM0489  CL94 HEK-293T C526(0.93)  LDD1692  [1]
 LDCM0490  CL95 HEK-293T C526(1.16); C579(0.91)  LDD1693  [1]
 LDCM0491  CL96 HEK-293T C526(1.21)  LDD1694  [1]
 LDCM0022  KB02 MGG123 C115(3.24)  LDD2469  [2]
 LDCM0023  KB03 MGG123 C115(4.71)  LDD2886  [2]
 LDCM0024  KB05 MGG123 C115(3.64)  LDD3303  [2]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840