Details of the Target

General Information of Target

Target ID LDTP02719
Target Name Interferon regulatory factor 2 (IRF2)
Gene Name IRF2
Gene ID 3660
Synonyms
Interferon regulatory factor 2; IRF-2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MPVERMRMRPWLEEQINSNTIPGLKWLNKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAI
HTGKHQPGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRMLPLSERPSK
KGKKPKTEKEDKVKHIKQEPVESSLGLSNGVSDLSPEYAVLTSTIKNEVDSTVNIIVVGQ
SHLDSNIENQEIVTNPPDICQVVEVTTESDEQPVSMSELYPLQISPVSSYAESETTDSVP
SDEESAEGRPHWRKRNIEGKQYLSNMGTRGSYLLPGMASFVTSNKPDLQVTIKEESNPVP
YNSSWPPFQDLPLSSSMTPASSSSRPDRETRASVIKKTSDITQARVKSC
Target Bioclass
Transcription factor
Family
IRF family
Subcellular location
Nucleus
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Uniprot ID
P14316
Ensemble ID
ENST00000393593.8
HGNC ID
HGNC:6117

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.T318A .
Ishikawa (Heraklio) 02 ER Deletion: p.N167MfsTer7
Insertion: p.Q35SfsTer9		 .
MCAS SNV: p.D96G .
MFE319 Insertion: p.N167KfsTer2
SNV: p.V177F		 .
PATU8988T SNV: p.S348C .
SUPT1 SNV: p.R117W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C83(2.38)  LDD3339  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0276  AC17 PaTu 8988t C83(1.01)  LDD1155  [3]
 LDCM0277  AC18 PaTu 8988t C83(1.30)  LDD1156  [3]
 LDCM0278  AC19 PaTu 8988t C83(1.04)  LDD1157  [3]
 LDCM0280  AC20 PaTu 8988t C83(1.27)  LDD1159  [3]
 LDCM0281  AC21 PaTu 8988t C83(1.14)  LDD1160  [3]
 LDCM0282  AC22 PaTu 8988t C83(1.29)  LDD1161  [3]
 LDCM0283  AC23 PaTu 8988t C83(1.24)  LDD1162  [3]
 LDCM0284  AC24 PaTu 8988t C83(1.20)  LDD1163  [3]
 LDCM0320  AC57 PaTu 8988t C83(1.24)  LDD1199  [3]
 LDCM0321  AC58 PaTu 8988t C83(1.50)  LDD1200  [3]
 LDCM0322  AC59 PaTu 8988t C83(1.16)  LDD1201  [3]
 LDCM0324  AC60 PaTu 8988t C83(1.45)  LDD1203  [3]
 LDCM0325  AC61 PaTu 8988t C83(1.24)  LDD1204  [3]
 LDCM0326  AC62 PaTu 8988t C83(1.11)  LDD1205  [3]
 LDCM0327  AC63 PaTu 8988t C83(1.26)  LDD1206  [3]
 LDCM0328  AC64 PaTu 8988t C83(1.26)  LDD1207  [3]
 LDCM0329  AC65 PaTu 8988t C83(1.18)  LDD1208  [3]
 LDCM0330  AC66 PaTu 8988t C83(1.25)  LDD1209  [3]
 LDCM0331  AC67 PaTu 8988t C83(1.06)  LDD1210  [3]
 LDCM0374  CL105 PaTu 8988t C83(1.14)  LDD1253  [3]
 LDCM0375  CL106 PaTu 8988t C83(1.00)  LDD1254  [3]
 LDCM0376  CL107 PaTu 8988t C83(1.00)  LDD1255  [3]
 LDCM0377  CL108 PaTu 8988t C83(1.21)  LDD1256  [3]
 LDCM0378  CL109 PaTu 8988t C83(1.34)  LDD1257  [3]
 LDCM0380  CL110 PaTu 8988t C83(1.35)  LDD1259  [3]
 LDCM0381  CL111 PaTu 8988t C83(1.55)  LDD1260  [3]
 LDCM0396  CL125 PaTu 8988t C83(1.20)  LDD1275  [3]
 LDCM0397  CL126 PaTu 8988t C83(1.23)  LDD1276  [3]
 LDCM0398  CL127 PaTu 8988t C83(0.98)  LDD1277  [3]
 LDCM0399  CL128 PaTu 8988t C83(1.14)  LDD1278  [3]
 LDCM0436  CL46 PaTu 8988t C83(1.01)  LDD1315  [3]
 LDCM0437  CL47 PaTu 8988t C83(1.02)  LDD1316  [3]
 LDCM0438  CL48 PaTu 8988t C83(0.99)  LDD1317  [3]
 LDCM0439  CL49 PaTu 8988t C83(1.04)  LDD1318  [3]
 LDCM0441  CL50 PaTu 8988t C83(1.08)  LDD1320  [3]
 LDCM0442  CL51 PaTu 8988t C83(1.00)  LDD1321  [3]
 LDCM0443  CL52 PaTu 8988t C83(1.09)  LDD1322  [3]
 LDCM0444  CL53 PaTu 8988t C83(1.09)  LDD1323  [3]
 LDCM0445  CL54 PaTu 8988t C83(0.99)  LDD1324  [3]
 LDCM0446  CL55 PaTu 8988t C83(1.21)  LDD1325  [3]
 LDCM0447  CL56 PaTu 8988t C83(0.98)  LDD1326  [3]
 LDCM0448  CL57 PaTu 8988t C83(1.09)  LDD1327  [3]
 LDCM0449  CL58 PaTu 8988t C83(1.12)  LDD1328  [3]
 LDCM0450  CL59 PaTu 8988t C83(1.06)  LDD1329  [3]
 LDCM0452  CL60 PaTu 8988t C83(0.95)  LDD1331  [3]
 LDCM0453  CL61 PaTu 8988t C83(1.06)  LDD1332  [3]
 LDCM0454  CL62 PaTu 8988t C83(0.87)  LDD1333  [3]
 LDCM0455  CL63 PaTu 8988t C83(0.88)  LDD1334  [3]
 LDCM0456  CL64 PaTu 8988t C83(0.95)  LDD1335  [3]
 LDCM0457  CL65 PaTu 8988t C83(0.98)  LDD1336  [3]
 LDCM0458  CL66 PaTu 8988t C83(0.94)  LDD1337  [3]
 LDCM0459  CL67 PaTu 8988t C83(1.05)  LDD1338  [3]
 LDCM0460  CL68 PaTu 8988t C83(1.46)  LDD1339  [3]
 LDCM0461  CL69 PaTu 8988t C83(1.15)  LDD1340  [3]
 LDCM0463  CL70 PaTu 8988t C83(1.13)  LDD1342  [3]
 LDCM0464  CL71 PaTu 8988t C83(0.80)  LDD1343  [3]
 LDCM0465  CL72 PaTu 8988t C83(0.75)  LDD1344  [3]
 LDCM0466  CL73 PaTu 8988t C83(1.09)  LDD1345  [3]
 LDCM0467  CL74 PaTu 8988t C83(1.02)  LDD1346  [3]
 LDCM0625  F8 Ramos C83(1.21)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C83(2.36)  LDD2189  [4]
 LDCM0573  Fragment11 Ramos C83(0.38)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C83(1.16)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C83(1.20)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C83(0.60)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C83(1.46)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C83(0.90)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C83(1.16)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C83(0.94)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C83(0.71)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C83(1.67)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C83(1.10)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C83(1.42)  LDD2201  [4]
 LDCM0468  Fragment33 PaTu 8988t C83(0.97)  LDD1347  [3]
 LDCM0596  Fragment38 Ramos C83(1.13)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C83(0.78)  LDD2184  [4]
 LDCM0610  Fragment52 Ramos C83(1.31)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C83(1.47)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C83(0.87)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C83(2.22)  LDD2188  [4]
 LDCM0022  KB02 HEK-293T C83(1.02)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C83(1.12)  LDD1497  [5]
 LDCM0024  KB05 NALM-6 C83(2.38)  LDD3339  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402