Details of the Target

General Information of Target

Target ID LDTP02452
Target Name Tubulin alpha-3C chain (TUBA3C)
Gene Name TUBA3C
Gene ID 7278
Synonyms
TUBA2; Tubulin alpha-3C chain; EC 3.6.5.-; Alpha-tubulin 2; Alpha-tubulin 3C; Tubulin alpha-2 chain) [Cleaved into: Detyrosinated tubulin alpha-3C chain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD
RIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDLAALEKDYEEVGVDSVEAEAEEGEEY
Target Bioclass
Enzyme
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Uniprot ID
P0DPH7
Ensemble ID
ENST00000400113.8
HGNC ID
HGNC:12408
ChEMBL ID
CHEMBL2095182

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.T361RfsTer18 .
IGR1 SNV: p.K124Ter .
JHH6 SNV: p.H28R .
JURKAT SNV: p.V409M .
LN18 SNV: p.Y210H .
MDST8 SNV: p.D98N .
MEWO SNV: p.A383V .
NALM6 Deletion: p.R264AfsTer81 .
NCIH1993 SNV: p.P37R .
PANC0403 SNV: p.K394T .
PATU8988T SNV: p.D116E .
REH SNV: p.R229C .
RVH421 SNV: p.S419F .
SKN SNV: p.A12P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
JZ128-DTB
 Probe Info  		C295(0.00); C376(0.00); C305(0.00)  LDD0462  [1]
THZ1-DTB
 Probe Info  		C347(1.14); C376(1.06); C295(1.05); C25(1.06)  LDD0460  [1]
HHS-475
 Probe Info  		Y103(0.64); Y399(0.65); Y262(0.67); Y272(0.77)  LDD0264  [2]
5E-2FA
 Probe Info  		H283(0.00); H266(0.00); H88(0.00); H61(0.00)  LDD2235  [3]
m-APA
 Probe Info  		H406(0.00); H283(0.00); H266(0.00); H88(0.00)  LDD2231  [3]
Ox-W18
 Probe Info  		W346(0.00); W388(0.00)  LDD2175  [4]
NAIA_5
 Probe Info  		C376(0.00); C305(0.00); C347(0.00); C4(0.00)  LDD2223  [5]
HHS-465
 Probe Info  		K280(0.00); K326(0.00); K336(0.00); K370(0.00)  LDD2240  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y103(0.64); Y399(0.65); Y262(0.67); Y272(0.77)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y399(0.53); Y161(0.53); Y103(0.60); Y262(0.60)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y262(0.52); Y399(0.61); Y272(0.64); Y103(0.67)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y272(0.39); Y262(0.44); Y399(0.49); Y103(0.54)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y103(0.40); Y272(0.47); Y262(0.52); Y399(0.55)  LDD0268  [2]
 LDCM0179  JZ128 PC-3 C295(0.00); C376(0.00); C305(0.00)  LDD0462  [1]
 LDCM0021  THZ1 HeLa S3 C347(1.14); C376(1.06); C295(1.05); C25(1.06)  LDD0460  [1]

References

1 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010