Details of the Target

General Information of Target

Target ID LDTP02237
Target Name DNA excision repair protein ERCC-1 (ERCC1)
Gene Name ERCC1
Gene ID 2067
Synonyms
DNA excision repair protein ERCC-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTY
AEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVP
WEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQ
ALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLT
TVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKARRLFDVLHEPFLKVP
Target Bioclass
Enzyme
Family
ERCC1/RAD10/SWI10 family
Subcellular location
Cytoplasm; Nucleus
Function
[Isoform 1]: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.; [Isoform 2]: Not functional in the nucleotide excision repair pathway.; [Isoform 3]: Not functional in the nucleotide excision repair pathway.; [Isoform 4]: Not functional in the nucleotide excision repair pathway.
Uniprot ID
P07992
Ensemble ID
ENST00000013807.9
HGNC ID
HGNC:3433
ChEMBL ID
CHEMBL3883316

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.P33H .
HT SNV: p.V126A .
JURKAT SNV: p.G95R Compound 10    Probe Info 
LNCaP clone FGC SNV: p.S66L .
MOLT4 SNV: p.S135I .
NCIH1155 SNV: p.A272S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0403  [1]
DBIA
 Probe Info  		C274(1.68)  LDD3339  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		C274(0.00); C238(0.00)  LDD0037  [3]
TFBX
 Probe Info  		N.A.  LDD0027  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
Compound 11
 Probe Info  		N.A.  LDD2213  [5]
IPM
 Probe Info  		N.A.  LDD2156  [6]
VSF
 Probe Info  		N.A.  LDD0007  [7]
Acrolein
 Probe Info  		C238(0.00); C274(0.00)  LDD0217  [8]
AOyne
 Probe Info  		15.00  LDD0443  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C238(0.95)  LDD1507  [11]
 LDCM0215  AC10 HEK-293T C274(1.01); C76(1.22)  LDD1508  [11]
 LDCM0226  AC11 HEK-293T C274(1.01); C76(1.13)  LDD1509  [11]
 LDCM0237  AC12 HEK-293T C274(1.17)  LDD1510  [11]
 LDCM0259  AC14 HEK-293T C274(0.99)  LDD1512  [11]
 LDCM0270  AC15 HEK-293T C76(1.05)  LDD1513  [11]
 LDCM0276  AC17 HEK-293T C238(1.01)  LDD1515  [11]
 LDCM0277  AC18 HEK-293T C274(1.03); C76(1.32)  LDD1516  [11]
 LDCM0278  AC19 HEK-293T C274(1.04); C76(0.94)  LDD1517  [11]
 LDCM0279  AC2 HEK-293T C274(1.08); C76(1.07)  LDD1518  [11]
 LDCM0280  AC20 HEK-293T C274(1.09)  LDD1519  [11]
 LDCM0282  AC22 HEK-293T C274(0.97)  LDD1521  [11]
 LDCM0283  AC23 HEK-293T C76(1.44)  LDD1522  [11]
 LDCM0285  AC25 HEK-293T C238(0.98)  LDD1524  [11]
 LDCM0286  AC26 HEK-293T C274(1.06); C76(1.16)  LDD1525  [11]
 LDCM0287  AC27 HEK-293T C274(0.94); C76(0.82)  LDD1526  [11]
 LDCM0288  AC28 HEK-293T C274(1.20)  LDD1527  [11]
 LDCM0290  AC3 HEK-293T C274(0.99); C76(1.10)  LDD1529  [11]
 LDCM0291  AC30 HEK-293T C274(1.08)  LDD1530  [11]
 LDCM0292  AC31 HEK-293T C76(1.18)  LDD1531  [11]
 LDCM0294  AC33 HEK-293T C238(1.02)  LDD1533  [11]
 LDCM0295  AC34 HEK-293T C274(0.93); C76(1.09)  LDD1534  [11]
 LDCM0296  AC35 HEK-293T C274(1.07); C76(1.37)  LDD1535  [11]
 LDCM0297  AC36 HEK-293T C274(1.18)  LDD1536  [11]
 LDCM0299  AC38 HEK-293T C274(1.07)  LDD1538  [11]
 LDCM0300  AC39 HEK-293T C76(1.04)  LDD1539  [11]
 LDCM0301  AC4 HEK-293T C274(1.27)  LDD1540  [11]
 LDCM0303  AC41 HEK-293T C238(1.02)  LDD1542  [11]
 LDCM0304  AC42 HEK-293T C274(1.01); C76(1.14)  LDD1543  [11]
 LDCM0305  AC43 HEK-293T C274(1.02); C76(1.24)  LDD1544  [11]
 LDCM0306  AC44 HEK-293T C274(1.20)  LDD1545  [11]
 LDCM0308  AC46 HEK-293T C274(1.07)  LDD1547  [11]
 LDCM0309  AC47 HEK-293T C76(1.00)  LDD1548  [11]
 LDCM0311  AC49 HEK-293T C238(0.99)  LDD1550  [11]
 LDCM0313  AC50 HEK-293T C274(1.03); C76(1.53)  LDD1552  [11]
 LDCM0314  AC51 HEK-293T C274(1.17); C76(0.89)  LDD1553  [11]
 LDCM0315  AC52 HEK-293T C274(1.19)  LDD1554  [11]
 LDCM0317  AC54 HEK-293T C274(1.05)  LDD1556  [11]
 LDCM0318  AC55 HEK-293T C76(1.13)  LDD1557  [11]
 LDCM0320  AC57 HEK-293T C238(1.05)  LDD1559  [11]
 LDCM0321  AC58 HEK-293T C274(1.02); C76(0.86)  LDD1560  [11]
 LDCM0322  AC59 HEK-293T C274(1.04); C76(1.07)  LDD1561  [11]
 LDCM0323  AC6 HEK-293T C274(0.93)  LDD1562  [11]
 LDCM0324  AC60 HEK-293T C274(1.11)  LDD1563  [11]
 LDCM0326  AC62 HEK-293T C274(1.01)  LDD1565  [11]
 LDCM0327  AC63 HEK-293T C76(1.28)  LDD1566  [11]
 LDCM0334  AC7 HEK-293T C76(1.07)  LDD1568  [11]
 LDCM0356  AKOS034007680 HEK-293T C238(1.05)  LDD1570  [11]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C238(0.00); C274(0.00)  LDD0222  [8]
 LDCM0367  CL1 HEK-293T C238(1.11); C274(0.93)  LDD1571  [11]
 LDCM0368  CL10 HEK-293T C274(1.04)  LDD1572  [11]
 LDCM0369  CL100 HEK-293T C274(1.01); C76(1.19)  LDD1573  [11]
 LDCM0370  CL101 HEK-293T C238(1.08); C274(0.95)  LDD1574  [11]
 LDCM0371  CL102 HEK-293T C76(1.08)  LDD1575  [11]
 LDCM0373  CL104 HEK-293T C274(1.02); C76(0.97)  LDD1577  [11]
 LDCM0374  CL105 HEK-293T C238(0.96); C274(0.95)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C76(0.93)  LDD1579  [11]
 LDCM0377  CL108 HEK-293T C274(1.06); C76(1.11)  LDD1581  [11]
 LDCM0378  CL109 HEK-293T C238(0.96); C274(1.00)  LDD1582  [11]
 LDCM0379  CL11 HEK-293T C76(1.02)  LDD1583  [11]
 LDCM0380  CL110 HEK-293T C76(0.78)  LDD1584  [11]
 LDCM0382  CL112 HEK-293T C274(0.99); C76(1.05)  LDD1586  [11]
 LDCM0383  CL113 HEK-293T C238(1.06); C274(0.93)  LDD1587  [11]
 LDCM0384  CL114 HEK-293T C76(0.73)  LDD1588  [11]
 LDCM0386  CL116 HEK-293T C274(1.04); C76(0.92)  LDD1590  [11]
 LDCM0387  CL117 HEK-293T C238(1.06); C274(0.97)  LDD1591  [11]
 LDCM0388  CL118 HEK-293T C76(1.16)  LDD1592  [11]
 LDCM0391  CL120 HEK-293T C274(1.12); C76(0.97)  LDD1595  [11]
 LDCM0392  CL121 HEK-293T C238(1.01); C274(0.96)  LDD1596  [11]
 LDCM0393  CL122 HEK-293T C76(0.96)  LDD1597  [11]
 LDCM0395  CL124 HEK-293T C274(1.02); C76(0.77)  LDD1599  [11]
 LDCM0396  CL125 HEK-293T C238(0.97); C274(0.95)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C76(0.94)  LDD1601  [11]
 LDCM0399  CL128 HEK-293T C274(1.11); C76(1.49)  LDD1603  [11]
 LDCM0400  CL13 HEK-293T C238(1.25); C274(0.96)  LDD1604  [11]
 LDCM0401  CL14 HEK-293T C76(0.97)  LDD1605  [11]
 LDCM0403  CL16 HEK-293T C274(1.03); C76(1.02)  LDD1607  [11]
 LDCM0404  CL17 HEK-293T C238(0.96)  LDD1608  [11]
 LDCM0405  CL18 HEK-293T C274(1.01); C76(1.22)  LDD1609  [11]
 LDCM0406  CL19 HEK-293T C274(1.19); C76(0.93)  LDD1610  [11]
 LDCM0407  CL2 HEK-293T C76(0.92)  LDD1611  [11]
 LDCM0408  CL20 HEK-293T C274(1.30)  LDD1612  [11]
 LDCM0410  CL22 HEK-293T C274(0.95)  LDD1614  [11]
 LDCM0411  CL23 HEK-293T C76(0.80)  LDD1615  [11]
 LDCM0413  CL25 HEK-293T C238(0.98); C274(0.90)  LDD1617  [11]
 LDCM0414  CL26 HEK-293T C76(1.10)  LDD1618  [11]
 LDCM0416  CL28 HEK-293T C274(0.98); C76(1.21)  LDD1620  [11]
 LDCM0417  CL29 HEK-293T C238(0.99)  LDD1621  [11]
 LDCM0419  CL30 HEK-293T C274(0.92); C76(1.21)  LDD1623  [11]
 LDCM0420  CL31 HEK-293T C274(1.16); C76(0.95)  LDD1624  [11]
 LDCM0421  CL32 HEK-293T C274(1.31)  LDD1625  [11]
 LDCM0423  CL34 HEK-293T C274(1.09)  LDD1627  [11]
 LDCM0424  CL35 HEK-293T C76(1.20)  LDD1628  [11]
 LDCM0426  CL37 HEK-293T C238(0.91); C274(0.84)  LDD1630  [11]
 LDCM0429  CL4 HEK-293T C274(1.06); C76(0.86)  LDD1633  [11]
 LDCM0430  CL40 HEK-293T C274(0.98); C76(1.05)  LDD1634  [11]
 LDCM0431  CL41 HEK-293T C238(1.01)  LDD1635  [11]
 LDCM0432  CL42 HEK-293T C274(1.14); C76(1.85)  LDD1636  [11]
 LDCM0433  CL43 HEK-293T C274(1.05); C76(0.90)  LDD1637  [11]
 LDCM0434  CL44 HEK-293T C274(1.04)  LDD1638  [11]
 LDCM0436  CL46 HEK-293T C274(1.09)  LDD1640  [11]
 LDCM0437  CL47 HEK-293T C76(1.03)  LDD1641  [11]
 LDCM0439  CL49 HEK-293T C238(1.06); C274(0.99)  LDD1643  [11]
 LDCM0440  CL5 HEK-293T C238(0.99)  LDD1644  [11]
 LDCM0441  CL50 HEK-293T C76(1.90)  LDD1645  [11]
 LDCM0443  CL52 HEK-293T C274(0.97); C76(0.96)  LDD1646  [11]
 LDCM0444  CL53 HEK-293T C238(0.93)  LDD1647  [11]
 LDCM0445  CL54 HEK-293T C274(0.97); C76(1.05)  LDD1648  [11]
 LDCM0446  CL55 HEK-293T C274(0.96); C76(0.74)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C274(1.17)  LDD1650  [11]
 LDCM0449  CL58 HEK-293T C274(1.10)  LDD1652  [11]
 LDCM0450  CL59 HEK-293T C76(1.00)  LDD1653  [11]
 LDCM0451  CL6 HEK-293T C274(1.04); C76(1.05)  LDD1654  [11]
 LDCM0453  CL61 HEK-293T C238(1.08); C274(1.04)  LDD1656  [11]
 LDCM0454  CL62 HEK-293T C76(1.06)  LDD1657  [11]
 LDCM0456  CL64 HEK-293T C274(1.08); C76(0.89)  LDD1659  [11]
 LDCM0457  CL65 HEK-293T C238(1.04)  LDD1660  [11]
 LDCM0458  CL66 HEK-293T C274(0.97); C76(0.96)  LDD1661  [11]
 LDCM0459  CL67 HEK-293T C274(0.97); C76(0.94)  LDD1662  [11]
 LDCM0460  CL68 HEK-293T C274(1.36)  LDD1663  [11]
 LDCM0462  CL7 HEK-293T C274(1.17); C76(0.87)  LDD1665  [11]
 LDCM0463  CL70 HEK-293T C274(1.05)  LDD1666  [11]
 LDCM0464  CL71 HEK-293T C76(1.16)  LDD1667  [11]
 LDCM0466  CL73 HEK-293T C238(1.08); C274(1.03)  LDD1669  [11]
 LDCM0467  CL74 HEK-293T C76(0.95)  LDD1670  [11]
 LDCM0469  CL76 HEK-293T C274(1.15); C76(0.96)  LDD1672  [11]
 LDCM0470  CL77 HEK-293T C238(0.89)  LDD1673  [11]
 LDCM0471  CL78 HEK-293T C274(1.13); C76(1.11)  LDD1674  [11]
 LDCM0472  CL79 HEK-293T C274(0.98); C76(0.83)  LDD1675  [11]
 LDCM0473  CL8 HEK-293T C274(1.17)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C274(1.10)  LDD1677  [11]
 LDCM0476  CL82 HEK-293T C274(1.06)  LDD1679  [11]
 LDCM0477  CL83 HEK-293T C76(0.89)  LDD1680  [11]
 LDCM0479  CL85 HEK-293T C238(1.11); C274(0.95)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C76(0.84)  LDD1683  [11]
 LDCM0482  CL88 HEK-293T C274(1.00); C76(1.18)  LDD1685  [11]
 LDCM0483  CL89 HEK-293T C238(0.94)  LDD1686  [11]
 LDCM0485  CL90 HEK-293T C274(0.90); C76(0.93)  LDD1688  [11]
 LDCM0486  CL91 HEK-293T C274(1.10); C76(0.98)  LDD1689  [11]
 LDCM0487  CL92 HEK-293T C274(1.13)  LDD1690  [11]
 LDCM0489  CL94 HEK-293T C274(1.02)  LDD1692  [11]
 LDCM0490  CL95 HEK-293T C76(1.09)  LDD1693  [11]
 LDCM0492  CL97 HEK-293T C238(1.00); C274(1.00)  LDD1695  [11]
 LDCM0493  CL98 HEK-293T C76(1.19)  LDD1696  [11]
 LDCM0427  Fragment51 HEK-293T C76(0.68)  LDD1631  [11]
 LDCM0022  KB02 8305C C274(2.58)  LDD2248  [2]
 LDCM0023  KB03 8305C C274(3.89)  LDD2665  [2]
 LDCM0024  KB05 NALM-6 C274(1.68)  LDD3339  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
tRNA (uracil-5-)-methyltransferase homolog B (TRMT2B) RNA M5U methyltransferase family Q96GJ1
Epidermal growth factor receptor (EGFR) Tyr protein kinase family P00533
DNA repair endonuclease XPF (ERCC4) XPF family Q92889
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dynein regulatory complex subunit 4 (GAS8) DRC4 family O95995
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
Structure-specific endonuclease subunit SLX4 (SLX4) SLX4 family Q8IY92
Vacuolar protein sorting-associated protein 37B (VPS37B) VPS37 family Q9H9H4
DNA repair protein complementing XP-A cells (XPA) XPA family P23025
Ubiquitin-like protein 7 (UBL7) . Q96S82

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402