Details of the Target

General Information of Target

Target ID LDTP01933
Target Name Spectrin alpha chain, erythrocytic 1 (SPTA1)
Gene Name SPTA1
Gene ID 6708
Synonyms
SPTA; Spectrin alpha chain, erythrocytic 1; Erythroid alpha-spectrin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKR
DADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFT
MGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAI
ATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQN
EVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASE
GLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALAT
SRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEID
SYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHL
FYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTAT
KLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINK
KKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTT
RLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLA
EVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKE
PLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILE
NIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQ
FQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRN
QANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAA
DHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQ
RYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKV
ADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIE
KKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDL
QRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAED
LTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDL
EKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLD
KAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYA
NLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVIN
LGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFE
FWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFN
VDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQD
YGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEH
WEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMK
HEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAW
KLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSF
QQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQK
AEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFK
CLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQ
EFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLG
DNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIY
KHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYT
AFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRG
RSHLSGYDYVGFTNSYFGN
Target Bioclass
Other
Family
Spectrin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Uniprot ID
P02549
Ensemble ID
ENST00000643759.2
HGNC ID
HGNC:11272

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.E2104K .
A549 SNV: p.E2295K .
CAL51 Deletion: p.R994EfsTer5 .
CALU1 SNV: p.Q645H .
CALU6 SNV: p.G301A .
CORL88 SNV: p.F1228Y .
DAOY SNV: p.A2198T .
DU145 Deletion: p.K728NfsTer29;p.K1369SfsTer7
SNV: p.R568S;p.L821H;p.H864N		 .
EFO21 SNV: p.Y1528Ter .
HCC1187 SNV: p.Q1581H .
HCT116 SNV: p.Q2108K .
HCT15 SNV: p.D424N; p.W465C .
HEC1 SNV: p.R1158Q; p.K1888R .
HEC1B SNV: p.R1158Q .
HGC27 SNV: p.L382F .
HT115 SNV: p.R34W; p.R470C; p.E1514Ter .
IGR1 SNV: p.A1288V .
JURKAT SNV: p.Q2035Ter .
JURLMK1 SNV: p.I1157S DBIA    Probe Info 
KELLY SNV: p.Q165K .
KMCH1 Deletion: p.R60QfsTer15
SNV: p.W1384L		 .
KMS27 SNV: p.K600N .
KYM1 SNV: p.A1945T .
KYSE410 SNV: p.R2028K .
LN229 SNV: p.L1328M .
LNCaP clone FGC Insertion: p.Q47SfsTer4
SNV: p.M70I;p.E241D;p.T635I;p.V1889A		 .
LOXIMVI SNV: p.G856E .
LUDLU1 SNV: p.Q2152K .
MCC13 SNV: p.S1252F; p.R1659Ter; p.E2312K .
MDAMB468 SNV: p.N1138S .
MDST8 SNV: p.E2347K .
MEWO SNV: p.R374Ter; p.G935E; p.E1908K; p.S1997F .
MFE296 SNV: p.Q1159H .
MM1S SNV: p.E1367Ter .
NCIH1703 Insertion: p.D1121EfsTer2
SNV: p.D1121Y		 .
NCIH2172 SNV: p.Y2179Ter .
NCIH2286 SNV: p.V899I .
NCIH2291 SNV: p.A2239S .
NCIH23 SNV: p.A2325P .
NCIH358 SNV: p.S2024Ter .
PC14 SNV: p.G1882Ter .
REH SNV: p.A2226T .
RL952 SNV: p.R411S .
RT112 SNV: p.G2267C .
SF295 Substitution: p.E23_I24delinsDV .
SH4 SNV: p.L1485H .
SHP77 SNV: p.S1676I .
SKMEL2 SNV: p.D1491N .
SW1116 SNV: p.Q1261K .
UACC62 SNV: p.Y1064C .
WM115 SNV: p.D705N .
WM2664 SNV: p.D705N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-EBA
 Probe Info  		2.67  LDD0215  [1]
STPyne
 Probe Info  		K1803(10.00)  LDD0277  [2]
DBIA
 Probe Info  		C773(1.36); C965(1.79); C1879(2.37)  LDD3334  [3]
Acrolein
 Probe Info  		N.A.  LDD0222  [4]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		5.28  LDD0472  [5]
FFF probe13
 Probe Info  		10.78  LDD0476  [5]
FFF probe3
 Probe Info  		14.70  LDD0465  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0022  KB02 EoL-1 C773(1.27); C2101(1.44); C1203(1.23)  LDD2324  [3]
 LDCM0023  KB03 EoL-1 C773(1.84); C2101(1.81)  LDD2741  [3]
 LDCM0024  KB05 MOLM-16 C773(1.36); C965(1.79); C1879(2.37)  LDD3334  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Branched-chain alpha-ketoacid dehydrogenase kinase (BCKDK) PDK/BCKDK protein kinase family O14874
Exosome complex component RRP46 (EXOSC5) RNase PH family Q9NQT4
Thioredoxin, mitochondrial (TXN2) Thioredoxin family Q99757
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Spectrin beta chain, non-erythrocytic 1 (SPTBN1) Spectrin family Q01082
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Teashirt homolog 2 (TSHZ2) Teashirt C2H2-type zinc-finger protein family Q9NRE2
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Abl interactor 1 (ABI1) ABI family Q8IZP0
DBIRD complex subunit ZNF326 (ZNF326) AKAP95 family Q5BKZ1
Protein DGCR6L (DGCR6L) Gonadal family Q9BY27
Keratin, type II cytoskeletal 75 (KRT75) Intermediate filament family O95678
Large ribosomal subunit protein mL64 (GADD45GIP1) Mitochondrion-specific ribosomal protein mL64 family Q8TAE8
Ciliary microtubule associated protein 1B (CIMAP1B) ODF3 family A8MYP8
Spectrin beta chain, erythrocytic (SPTB) Spectrin family P11277
Coiled-coil domain-containing protein 185 (CCDC185) . Q8N715

References

1 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.