Details of the Target

General Information of Target

Target ID LDTP01611
Target Name Formin-like protein 1 (FMNL1)
Gene Name FMNL1
Gene ID 752
Synonyms
C17orf1; C17orf1B; FMNL; FRL1; Formin-like protein 1; CLL-associated antigen KW-13; Leukocyte formin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGNAAGSAEQPAGPAAPPPKQPAPPKQPMPAAGELEERFNRALNCMNLPPDKVQLLSQYD
NEKKWELICDQERFQVKNPPAAYIQKLKSYVDTGGVSRKVAADWMSNLGFKRRVQESTQV
LRELETSLRTNHIGWVQEFLNEENRGLDVLLEYLAFAQCSVTYDMESTDNGASNSEKNKP
LEQSVEDLSKGPPSSVPKSRHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNY
QSGFSLVMNHPACVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVC
GEQHRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLE
RLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAEN
ESMAKIAELEKQLSQARKELETLRERFSESTAMGPSRRPPEPEKAPPAAPTRPSALELKV
EELEEKGLIRILRGPGDAVSIEILPVAVATPSGGDAPTPGVPTGSPSPDLAPAAEPAPGA
APPPPPPLPGLPSPQEAPPSAPPQAPPLPGSPEPPPAPPLPGDLPPPPPPPPPPPGTDGP
VPPPPPPPPPPPGGPPDALGRRDSELGPGVKAKKPIQTKFRMPLLNWVALKPSQITGTVF
TELNDEKVLQELDMSDFEEQFKTKSQGPSLDLSALKSKAAQKAPSKATLIEANRAKNLAI
TLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFEREQRPMEELSE
EDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEI
VLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSD
LHFLDKAGSVSLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADS
KTAQEAFESVVEYFGENPKTTSPGLFFSLFSRFIKAYKKAEQEVEQWKKEAAAQEAGADT
PGKGEPPAPKSPPKARRPQMDLISELKRRQQKEPLIYESDRDGAIEDIITVIKTVPFTAR
TGKRTSRLLCEASLGEEMPL
Target Type
Literature-reported
Target Bioclass
Other
Family
Formin homology family
Subcellular location
Cytoplasm; Cytoplasm, cell cortex
Function
May play a role in the control of cell motility and survival of macrophages. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
TTD ID
T95154
Uniprot ID
O95466
DrugMap ID
TTW20PQ
Ensemble ID
ENST00000331495.8
HGNC ID
HGNC:1212

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.M46I DBIA    Probe Info 
CAMA1 SNV: p.S560F DBIA    Probe Info 
DOTC24510 SNV: p.E965G .
HCT116 Deletion: p.S527AfsTer93 .
HT SNV: p.M46T DBIA    Probe Info 
IGROV1 SNV: p.Q680P .
Ishikawa (Heraklio) 02 ER SNV: p.G243D DBIA    Probe Info 
LS180 Deletion: p.A729PfsTer24 DBIA    Probe Info 
MCC13 SNV: p.S336L DBIA    Probe Info 
MOLT4 SNV: p.S184F; p.H304Y; p.A507T; p.L756M; p.A866E; p.R920H IA-alkyne    Probe Info 
NALM6 SNV: p.F39L DBIA    Probe Info 
NCIH1155 SNV: p.R1061C DBIA    Probe Info 
OVISE SNV: p.Q564P .
SNGM SNV: p.A649T DBIA    Probe Info 
SNU5 SNV: p.P543Q DBIA    Probe Info 
SUPT1 SNV: p.R414W DBIA    Probe Info 
TF1 SNV: p.P545T DBIA    Probe Info 
TOV21G SNV: p.S474Ter; p.M798V DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y90(10.61)  LDD0260  [1]
ONAyne
 Probe Info  		K702(0.88)  LDD0274  [2]
STPyne
 Probe Info  		K1047(10.00); K111(2.00); K696(10.00); K77(0.63)  LDD0277  [2]
BTD
 Probe Info  		C300(0.43)  LDD2091  [3]
HPAP
 Probe Info  		3.15  LDD0064  [4]
Alkyne-RA190
 Probe Info  		2.09  LDD0300  [5]
DBIA
 Probe Info  		C787(8.19); C787(199.02); C300(14.46)  LDD0209  [6]
IPM
 Probe Info  		C69(1.95)  LDD1702  [3]
N1
 Probe Info  		N.A.  LDD0245  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C253(0.00); C69(0.00); C787(0.00); C939(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		C253(0.00); C787(0.00); C69(0.00); C939(0.00)  LDD0036  [8]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [9]
Lodoacetamide azide
 Probe Info  		C45(0.00); C69(0.00); C253(0.00); C787(0.00)  LDD0037  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C300(1.21)  LDD2117  [3]
 LDCM0498  BS-3668 MDA-MB-231 C300(0.43)  LDD2091  [3]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C69(1.95)  LDD1702  [3]
 LDCM0022  KB02 T cell C939(12.25)  LDD1707  [10]
 LDCM0023  KB03 Jurkat C787(8.19); C787(199.02); C300(14.46)  LDD0209  [6]
 LDCM0024  KB05 IGR37 C300(1.51)  LDD3314  [11]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C300(1.28)  LDD2099  [3]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C300(0.67)  LDD2104  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C300(0.90)  LDD2109  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C300(0.95)  LDD2111  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C300(0.95)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C300(1.06)  LDD2127  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C300(0.94)  LDD2129  [3]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C300(0.79)  LDD2133  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C300(0.58)  LDD2134  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C300(1.14)  LDD2137  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C300(1.11)  LDD2146  [3]
 LDCM0014  Panhematin hPBMC 3.15  LDD0064  [4]
 LDCM0131  RA190 MM1.R 2.09  LDD0300  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
High mobility group protein 20A (HMG20A) . Q9NP66
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) . O75044

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
5 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
6 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
7 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
10 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840