Details of the Target

General Information of Target

Target ID LDTP00995
Target Name Beta-1,4-galactosyltransferase 4 (B4GALT4)
Gene Name B4GALT4
Gene ID 8702
Synonyms
Beta-1,4-galactosyltransferase 4; Beta-1,4-GalTase 4; Beta4Gal-T4; b4Gal-T4; EC 2.4.1.-; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Lactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.275; N-acetyllactosamine synthase; EC 2.4.1.90; Nal synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGFNLTFHLSYKFRLLLLLTLCLTVVGWATSNYFVGAIQEIPKAKEFMANFHKTLILGKG
KTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPQECKA
LQRVAILVPHRNREKHLMYLLEHLHPFLQRQQLDYGIYVIHQAEGKKFNRAKLLNVGYLE
ALKEENWDCFIFHDVDLVPENDFNLYKCEEHPKHLVVGRNSTGYRLRYSGYFGGVTALSR
EQFFKVNGFSNNYWGWGGEDDDLRLRVELQRMKISRPLPEVGKYTMVFHTRDKGNEVNAE
RMKLLHQVSRVWRTDGLSSCSYKLVSVEHNPLYINITVDFWFGA
Target Bioclass
Enzyme
Family
Glycosyltransferase 7 family
Subcellular location
Golgi apparatus membrane
Function
Galactose (Gal) transferase involved in the synthesis of terminal N-acetyllactosamine (LacNac) unit present on glycan chains of glycoproteins and glycosphingolipids. Catalyzes the transfer of Gal residue via a beta1->4 linkage from UDP-Gal to the non-reducing terminal N-acetyl glucosamine 6-O-sulfate (6-O-sulfoGlcNAc) in the linearly growing chain of both N- and O-linked keratan sulfate proteoglycans. Cooperates with B3GNT7 N-acetyl glucosamine transferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer. Transfers Gal residue via a beta1->4 linkage to terminal 6-O-sulfoGlcNAc within the LacNac unit of core 2 O-glycans forming 6-sulfo-sialyl-Lewis X (sLex). May contribute to the generation of sLex epitope on mucin-type glycoproteins that serve as ligands for SELL/L-selectin, a major regulator of leukocyte migration. In the biosynthesis pathway of neolacto-series glycosphingolipids, transfers Gal residue via a beta1->4 linkage to terminal GlcNAc of a lactotriaosylceramide (Lc3Cer) acceptor to form a neolactotetraosylceramide.
Uniprot ID
O60513
Ensemble ID
ENST00000359213.7
HGNC ID
HGNC:927

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C208(4.00)  LDD3342  [1]
BTD
 Probe Info  		C118(0.67)  LDD2111  [2]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 PaTu 8988t C320(0.61)  LDD1094  [4]
 LDCM0226  AC11 PaTu 8988t C320(0.52)  LDD1105  [4]
 LDCM0237  AC12 PaTu 8988t C320(0.61)  LDD1116  [4]
 LDCM0259  AC14 PaTu 8988t C320(0.52)  LDD1138  [4]
 LDCM0270  AC15 PaTu 8988t C320(0.59)  LDD1149  [4]
 LDCM0276  AC17 PaTu 8988t C320(0.87)  LDD1155  [4]
 LDCM0277  AC18 PaTu 8988t C320(0.68)  LDD1156  [4]
 LDCM0278  AC19 PaTu 8988t C320(0.72)  LDD1157  [4]
 LDCM0280  AC20 PaTu 8988t C320(1.16)  LDD1159  [4]
 LDCM0281  AC21 PaTu 8988t C320(0.83)  LDD1160  [4]
 LDCM0282  AC22 PaTu 8988t C320(0.82)  LDD1161  [4]
 LDCM0283  AC23 PaTu 8988t C320(0.53)  LDD1162  [4]
 LDCM0284  AC24 PaTu 8988t C320(0.65)  LDD1163  [4]
 LDCM0287  AC27 HEK-293T C77(1.00)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C320(1.21)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C77(1.10); C118(0.92)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C77(1.07)  LDD1529  [5]
 LDCM0296  AC35 HEK-293T C77(1.08)  LDD1535  [5]
 LDCM0297  AC36 HEK-293T C320(1.15)  LDD1536  [5]
 LDCM0298  AC37 HEK-293T C77(1.03); C118(0.94)  LDD1537  [5]
 LDCM0301  AC4 HEK-293T C320(1.12)  LDD1540  [5]
 LDCM0305  AC43 HEK-293T C77(1.08)  LDD1544  [5]
 LDCM0306  AC44 HEK-293T C320(1.26)  LDD1545  [5]
 LDCM0307  AC45 HEK-293T C77(1.15); C118(0.96)  LDD1546  [5]
 LDCM0312  AC5 HEK-293T C77(1.18); C118(0.93)  LDD1551  [5]
 LDCM0314  AC51 HEK-293T C77(1.00)  LDD1553  [5]
 LDCM0315  AC52 HEK-293T C320(1.21)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C77(1.09); C118(0.96)  LDD1555  [5]
 LDCM0322  AC59 HEK-293T C77(1.03)  LDD1561  [5]
 LDCM0323  AC6 PaTu 8988t C320(0.82)  LDD1202  [4]
 LDCM0324  AC60 HEK-293T C320(1.10)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C77(1.09); C118(0.98)  LDD1564  [5]
 LDCM0334  AC7 PaTu 8988t C320(0.92)  LDD1213  [4]
 LDCM0345  AC8 PaTu 8988t C320(0.77)  LDD1224  [4]
 LDCM0248  AKOS034007472 PaTu 8988t C320(0.48)  LDD1127  [4]
 LDCM0356  AKOS034007680 PaTu 8988t C320(0.87)  LDD1235  [4]
 LDCM0275  AKOS034007705 PaTu 8988t C320(0.66)  LDD1154  [4]
 LDCM0367  CL1 PaTu 8988t C320(0.62)  LDD1246  [4]
 LDCM0368  CL10 PaTu 8988t C320(0.49)  LDD1247  [4]
 LDCM0369  CL100 HEK-293T C320(0.93)  LDD1573  [5]
 LDCM0370  CL101 PaTu 8988t C320(0.81)  LDD1249  [4]
 LDCM0371  CL102 PaTu 8988t C320(0.88)  LDD1250  [4]
 LDCM0372  CL103 PaTu 8988t C320(0.52)  LDD1251  [4]
 LDCM0373  CL104 PaTu 8988t C320(0.72)  LDD1252  [4]
 LDCM0374  CL105 PaTu 8988t C320(0.74)  LDD1253  [4]
 LDCM0375  CL106 PaTu 8988t C320(0.84)  LDD1254  [4]
 LDCM0376  CL107 PaTu 8988t C320(0.96)  LDD1255  [4]
 LDCM0377  CL108 PaTu 8988t C320(1.60)  LDD1256  [4]
 LDCM0378  CL109 PaTu 8988t C320(0.57)  LDD1257  [4]
 LDCM0379  CL11 PaTu 8988t C320(0.56)  LDD1258  [4]
 LDCM0380  CL110 PaTu 8988t C320(0.47)  LDD1259  [4]
 LDCM0381  CL111 PaTu 8988t C320(0.44)  LDD1260  [4]
 LDCM0382  CL112 HEK-293T C320(1.10)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C320(1.02)  LDD1587  [5]
 LDCM0386  CL116 HEK-293T C320(1.11)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C320(1.03)  LDD1591  [5]
 LDCM0390  CL12 PaTu 8988t C320(0.52)  LDD1269  [4]
 LDCM0391  CL120 HEK-293T C320(1.00)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C320(1.07)  LDD1596  [5]
 LDCM0395  CL124 HEK-293T C320(1.12)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C320(1.28)  LDD1600  [5]
 LDCM0399  CL128 HEK-293T C320(1.02)  LDD1603  [5]
 LDCM0400  CL13 PaTu 8988t C320(0.41)  LDD1279  [4]
 LDCM0401  CL14 PaTu 8988t C320(0.40)  LDD1280  [4]
 LDCM0402  CL15 PaTu 8988t C320(0.50)  LDD1281  [4]
 LDCM0403  CL16 HEK-293T C320(1.06)  LDD1607  [5]
 LDCM0406  CL19 HEK-293T C77(0.98)  LDD1610  [5]
 LDCM0407  CL2 PaTu 8988t C320(0.74)  LDD1286  [4]
 LDCM0408  CL20 HEK-293T C320(1.26)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C77(1.03); C118(1.10)  LDD1613  [5]
 LDCM0413  CL25 HEK-293T C320(1.07)  LDD1617  [5]
 LDCM0416  CL28 HEK-293T C320(1.02)  LDD1620  [5]
 LDCM0418  CL3 PaTu 8988t C320(0.75)  LDD1297  [4]
 LDCM0420  CL31 PaTu 8988t C320(0.88)  LDD1299  [4]
 LDCM0421  CL32 PaTu 8988t C320(0.58)  LDD1300  [4]
 LDCM0422  CL33 PaTu 8988t C320(0.52)  LDD1301  [4]
 LDCM0423  CL34 PaTu 8988t C320(0.49)  LDD1302  [4]
 LDCM0424  CL35 PaTu 8988t C320(0.46)  LDD1303  [4]
 LDCM0425  CL36 PaTu 8988t C320(0.45)  LDD1304  [4]
 LDCM0426  CL37 PaTu 8988t C320(0.45)  LDD1305  [4]
 LDCM0428  CL39 PaTu 8988t C320(0.75)  LDD1307  [4]
 LDCM0429  CL4 PaTu 8988t C320(0.60)  LDD1308  [4]
 LDCM0430  CL40 PaTu 8988t C320(0.57)  LDD1309  [4]
 LDCM0431  CL41 PaTu 8988t C320(0.50)  LDD1310  [4]
 LDCM0432  CL42 PaTu 8988t C320(0.43)  LDD1311  [4]
 LDCM0433  CL43 PaTu 8988t C320(0.50)  LDD1312  [4]
 LDCM0434  CL44 PaTu 8988t C320(0.46)  LDD1313  [4]
 LDCM0435  CL45 PaTu 8988t C320(0.72)  LDD1314  [4]
 LDCM0439  CL49 HEK-293T C320(1.24)  LDD1643  [5]
 LDCM0440  CL5 PaTu 8988t C320(0.48)  LDD1319  [4]
 LDCM0443  CL52 HEK-293T C320(1.02)  LDD1646  [5]
 LDCM0446  CL55 HEK-293T C77(0.99)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C320(1.37)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C77(1.02); C118(1.11)  LDD1651  [5]
 LDCM0451  CL6 PaTu 8988t C320(0.42)  LDD1330  [4]
 LDCM0453  CL61 PaTu 8988t C320(1.52)  LDD1332  [4]
 LDCM0454  CL62 PaTu 8988t C320(0.52)  LDD1333  [4]
 LDCM0455  CL63 PaTu 8988t C320(0.58)  LDD1334  [4]
 LDCM0456  CL64 PaTu 8988t C320(0.54)  LDD1335  [4]
 LDCM0457  CL65 PaTu 8988t C320(0.87)  LDD1336  [4]
 LDCM0458  CL66 PaTu 8988t C320(0.50)  LDD1337  [4]
 LDCM0459  CL67 PaTu 8988t C320(0.51)  LDD1338  [4]
 LDCM0460  CL68 PaTu 8988t C320(0.61)  LDD1339  [4]
 LDCM0461  CL69 PaTu 8988t C320(0.49)  LDD1340  [4]
 LDCM0462  CL7 PaTu 8988t C320(0.33)  LDD1341  [4]
 LDCM0463  CL70 PaTu 8988t C320(0.68)  LDD1342  [4]
 LDCM0464  CL71 PaTu 8988t C320(0.70)  LDD1343  [4]
 LDCM0465  CL72 PaTu 8988t C320(0.91)  LDD1344  [4]
 LDCM0466  CL73 PaTu 8988t C320(0.52)  LDD1345  [4]
 LDCM0467  CL74 PaTu 8988t C320(0.69)  LDD1346  [4]
 LDCM0469  CL76 HEK-293T C320(1.00)  LDD1672  [5]
 LDCM0472  CL79 HEK-293T C77(0.96)  LDD1675  [5]
 LDCM0473  CL8 PaTu 8988t C320(0.65)  LDD1352  [4]
 LDCM0474  CL80 HEK-293T C320(1.15)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C77(1.01); C118(0.96)  LDD1678  [5]
 LDCM0479  CL85 HEK-293T C320(1.31)  LDD1682  [5]
 LDCM0482  CL88 HEK-293T C320(1.04)  LDD1685  [5]
 LDCM0484  CL9 PaTu 8988t C320(0.35)  LDD1363  [4]
 LDCM0486  CL91 HEK-293T C77(1.01)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C320(1.39)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C77(1.04); C118(1.01)  LDD1691  [5]
 LDCM0492  CL97 HEK-293T C320(1.20)  LDD1695  [5]
 LDCM0468  Fragment33 PaTu 8988t C320(0.65)  LDD1347  [4]
 LDCM0427  Fragment51 PaTu 8988t C320(0.52)  LDD1306  [4]
 LDCM0022  KB02 22RV1 C77(2.41)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C77(4.38)  LDD2660  [1]
 LDCM0024  KB05 NCI-H1155 C208(4.00)  LDD3342  [1]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C118(0.67)  LDD2111  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C118(0.80)  LDD2127  [2]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
N-acetylglucosamine Small molecular drug DB00141

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402