Details of the Target

General Information of Target

Target ID LDTP00554
Target Name Protein Mdm4 (MDM4)
Gene Name MDM4
Gene ID 4194
Synonyms
MDMX; Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYI
MVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTL
ALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQ
DETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWF
LNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTS
EDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVP
DCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQ
RTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKE
IQLVIKVFIA
Target Type
Clinical trial
Target Bioclass
Other
Family
MDM2/MDM4 family
Subcellular location
Nucleus
Function
Along with MDM2, contributes to TP53 regulation. Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
TTD ID
T36741
Uniprot ID
O15151
DrugMap ID
TT9OUDQ
Ensemble ID
ENST00000367182.8
HGNC ID
HGNC:6974
ChEMBL ID
CHEMBL1255126

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.V267A .
HCT116 Deletion: p.N281MfsTer10 .
HCT15 SNV: p.C76Y .
HT115 SNV: p.D233Y .
Ishikawa (Heraklio) 02 ER SNV: p.E428K .
JURKAT SNV: p.M427I .
KYSE410 SNV: p.R322C .
NCIH1155 SNV: p.T422I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C334(0.98)  LDD0531  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C334(0.98)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C334(1.32)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C334(0.76)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C334(0.76)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C334(0.77)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C334(1.22)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C334(0.81)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C334(1.10)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C334(1.11)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C334(0.78)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C334(0.66)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C334(0.89)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C334(0.71)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C334(0.76)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C334(1.02)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C334(0.70)  LDD0546  [1]
 LDCM0237  AC12 HCT 116 C334(0.96)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C334(0.81)  LDD0576  [1]
 LDCM0270  AC15 HCT 116 C334(0.95)  LDD0587  [1]
 LDCM0276  AC17 HCT 116 C334(0.89)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C334(1.31)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C334(0.94)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C334(1.14)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C334(0.98)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C334(0.89)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C334(1.03)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C334(1.03)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C334(0.81)  LDD0601  [1]
 LDCM0290  AC3 HCT 116 C334(1.12)  LDD0607  [1]
 LDCM0301  AC4 HCT 116 C334(1.12)  LDD0618  [1]
 LDCM0312  AC5 HCT 116 C334(1.14)  LDD0629  [1]
 LDCM0323  AC6 HCT 116 C334(0.89)  LDD0640  [1]
 LDCM0334  AC7 HCT 116 C334(0.91)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C334(1.39)  LDD0662  [1]
 LDCM0365  AC98 HCT 116 C334(0.76)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C334(0.76)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C334(0.76)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C334(1.42)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C334(2.04)  LDD0592  [1]
 LDCM0369  CL100 HCT 116 C334(1.07)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C334(1.07)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C334(1.13)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C334(1.30)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C334(1.00)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C334(1.17)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C334(1.15)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C334(1.24)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C334(1.26)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C334(1.02)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C334(1.32)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C334(1.36)  LDD0698  [1]
 LDCM0436  CL46 HCT 116 C334(0.96)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C334(0.94)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C334(1.06)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C334(0.96)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C334(0.99)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C334(1.00)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C334(0.98)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C334(0.95)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C334(0.96)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C334(0.93)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C334(0.99)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C334(0.95)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C334(1.00)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C334(0.88)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C334(0.96)  LDD0769  [1]
 LDCM0486  CL91 HCT 116 C334(0.95)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C334(1.48)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C334(1.70)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C334(1.44)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C334(1.05)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C334(1.36)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C334(1.21)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C334(0.91)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C334(1.20)  LDD0811  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
N-alpha-acetyltransferase 10 (NAA10) Acetyltransferase family P41227
E3 ubiquitin-protein ligase Mdm2 (MDM2) MDM2/MDM4 family Q00987
Ubiquitin carboxyl-terminal hydrolase 7 (USP7) Peptidase C19 family Q93009
Casein kinase I isoform alpha (CSNK1A1) CK1 Ser/Thr protein kinase family P48729
Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) Ubiquitin-conjugating enzyme family P62837
E3 ubiquitin-protein ligase RNF115 (RNF115) . Q9Y4L5
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Transporter and channel
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein epsilon (YWHAE) 14-3-3 family P62258
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Apoptosis regulator Bcl-2 (BCL2) Bcl-2 family P10415
Cellular tumor antigen p53 (TP53) P53 family P04637
Protein S100-A1 (S100A1) S-100 family P23297
Protein S100-B (S100B) S-100 family P04271
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) Krueppel C2H2-type zinc-finger protein family Q9BRR0
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CD160 antigen (CD160) . O95971
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Retinoblastoma-associated protein (RB1) Retinoblastoma protein (RB) family P06400
Protein S100-A2 (S100A2) S-100 family P29034
Protein S100-A5 (S100A5) S-100 family P33763
Vacuolar protein sorting-associated protein 26C (VPS26C) VPS26 family O14972
WD repeat-containing protein 5 (WDR5) WD repeat WDR5/wds family P61964
AFG2-interacting ribosome maturation factor (AIRIM) . Q9NX04

The Drug(s) Related To This Target

Phase 2
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Alrn-6924 . D07UKT
Preclinical
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nsc-207895 Small molecular drug DPBE75

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.