Details of the Target

General Information of Target

Target ID LDTP17268
Target Name ATP synthase subunit epsilon-like protein, mitochondrial (ATP5F1EP2)
Gene Name ATP5F1EP2
Synonyms
ATP5EP2; ATP synthase subunit epsilon-like protein, mitochondrial; ATP synthase F1 subunit epsilon pseudogene 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFEDVFSDSGNTGNFDRGKKRRLTIIECGCDINMMIDLAKVADLVLMLIDASFGFEMEMF
EFLNICQAHGFPKILGVLTHLDSFKHNKQLKKTKKRLKHRFWTEVYQDKVGLTHELVQSL
ISTYSTIDAKMASSRVTLLSNSKPLGSEAIDNQGVSLEFDQQQGSVCPSESEIYEAGAED
RMAGAPMAAAVQPAEVTVEVGEDLHMHQVRDREMPEVVEIRRSNCTNHCDLGDTSSYHTK
VSTVHIMKKRNGGGSLNNYSSSIPPTPSTSQEDPQFSVPPTANTPTPVCKRSMRWSNLFT
SEKGSDPDKERKAPENHADTIGSGRAIPIKQGMLLKRSGKWLKTWKKKYVTLCSNGVLTY
YSSLGDYMKNIHKKEIDLRTSTIKVPGKWPSLATSACAPISSSKSNGLSKDMDTGLGDSI
CFSPGISSTTSPKLNPPPSPHANKKKHLKKKSTNNFMIVSATGQTWHFEATTYEERDAWV
QAIQSQILASLQSCKSSKSKSQLTSQSEAMALQSIQNMRGNAHCVDCETQNPKWASLNLG
VLMCIECSGIHRSFGTRLSRVRSLELDDWPVELRKVMSSIGNELANSIWEGSSQGQTKPS
IKSTREEKEWWIRSKYEEKLFLAPLPCTELSLGQQLLRATTDEDLQTAILLLAHGSREEV
NETCGEGDGCTALHLACRKGNVVLEQLLTGWTSWPEMPTGTQR
Target Bioclass
Enzyme
Family
Eukaryotic ATPase epsilon family
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Uniprot ID
Q5VTU8
HGNC ID
HGNC:34026

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C19(0.87)  LDD1700  [1]
DBIA
 Probe Info  		C19(1.01)  LDD0078  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [3]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [5]
TFBX
 Probe Info  		N.A.  LDD0027  [4]
IPM
 Probe Info  		N.A.  LDD0005  [6]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
Cinnamaldehyde
 Probe Info  		N.A.  LDD0220  [8]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [8]
Methacrolein
 Probe Info  		N.A.  LDD0218  [8]
W1
 Probe Info  		N.A.  LDD0236  [9]
AOyne
 Probe Info  		13.00  LDD0443  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C19(0.53)  LDD2142  [1]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C19(0.79)  LDD2112  [1]
 LDCM0545  Acetamide MDA-MB-231 C19(3.02)  LDD2138  [1]
 LDCM0520  AKOS000195272 MDA-MB-231 C19(1.74)  LDD2113  [1]
 LDCM0020  ARS-1620 HCC44 C19(1.01)  LDD0078  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [8]
 LDCM0022  KB02 22RV1 C19(1.36)  LDD2243  [11]
 LDCM0023  KB03 22RV1 C19(1.77)  LDD2660  [11]
 LDCM0024  KB05 Hs 936.T C19(1.68)  LDD3313  [11]
 LDCM0109  NEM HeLa N.A.  LDD0223  [8]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C19(1.03)  LDD2093  [1]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C19(0.13)  LDD2096  [1]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C19(2.95)  LDD2097  [1]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C19(0.46)  LDD2100  [1]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C19(1.12)  LDD2101  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C19(0.54)  LDD2104  [1]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C19(0.55)  LDD2108  [1]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C19(1.20)  LDD2110  [1]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C19(1.08)  LDD2111  [1]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C19(0.91)  LDD2114  [1]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C19(1.27)  LDD2115  [1]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C19(0.41)  LDD2118  [1]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C19(0.13)  LDD2124  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C19(5.06)  LDD2125  [1]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C19(0.14)  LDD2126  [1]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C19(0.92)  LDD2133  [1]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C19(1.62)  LDD2134  [1]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C19(5.68)  LDD2135  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C19(2.91)  LDD2136  [1]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C19(0.87)  LDD1700  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C19(0.46)  LDD2141  [1]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C19(1.32)  LDD2143  [1]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C19(0.25)  LDD2145  [1]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C19(0.69)  LDD2148  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840