Details of the Target

General Information of Target

Target ID LDTP15397
Target Name Cytochrome P450 4X1 (CYP4X1)
Gene Name CYP4X1
Gene ID 260293
Synonyms
Cytochrome P450 4X1; EC 1.14.14.-; CYPIVX1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPSLAPDCPLLAMPEETQEDSVAPMMPSQRSRGPLAPNHVHEVCLHQVESISDLHSGAGT
LRPYLTEEARPWDELLGVLPPSLCAQAGCSPVYRRGGFLLLLALLVLTCLVLALLAVYLS
VLQSESLRILAHTLRTQEETLLKLRLASLSQLRRLNSSEAQAPS
Target Bioclass
Enzyme
Family
Cytochrome P450 family
Subcellular location
Endoplasmic reticulum membrane
Function
A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Uniprot ID
Q8N118
Ensemble ID
ENST00000371901.4
HGNC ID
HGNC:20244
ChEMBL ID
CHEMBL6048

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.K390N .
IGR1 SNV: p.E168K; p.H225Y .
LS123 SNV: p.L24R .
MEWO Substitution: p.S268F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C203(0.78)  LDD1492  [1]
CY4
 Probe Info  		N.A.  LDD0247  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C203(1.03)  LDD1523  [1]
 LDCM0293  AC32 HEK-293T C203(0.99)  LDD1532  [1]
 LDCM0302  AC40 HEK-293T C203(0.80)  LDD1541  [1]
 LDCM0310  AC48 HEK-293T C203(0.99)  LDD1549  [1]
 LDCM0319  AC56 HEK-293T C203(0.91)  LDD1558  [1]
 LDCM0328  AC64 HEK-293T C203(0.81)  LDD1567  [1]
 LDCM0345  AC8 HEK-293T C203(1.13)  LDD1569  [1]
 LDCM0275  AKOS034007705 HEK-293T C203(0.76)  LDD1514  [1]
 LDCM0390  CL12 HEK-293T C203(1.05)  LDD1594  [1]
 LDCM0412  CL24 HEK-293T C203(1.08)  LDD1616  [1]
 LDCM0425  CL36 HEK-293T C203(1.05)  LDD1629  [1]
 LDCM0438  CL48 HEK-293T C203(1.23)  LDD1642  [1]
 LDCM0452  CL60 HEK-293T C203(1.22)  LDD1655  [1]
 LDCM0465  CL72 HEK-293T C203(1.26)  LDD1668  [1]
 LDCM0478  CL84 HEK-293T C203(1.24)  LDD1681  [1]
 LDCM0491  CL96 HEK-293T C203(0.77)  LDD1694  [1]
 LDCM0022  KB02 HEK-293T C203(0.78)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C203(0.94)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C203(0.94)  LDD1502  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.