Details of the Target

General Information of Target

Target ID LDTP15108
Target Name Large ribosomal subunit protein uL14m (MRPL14)
Gene Name MRPL14
Gene ID 64928
Synonyms
MRPL32; RPML32; Large ribosomal subunit protein uL14m; 39S ribosomal protein L14, mitochondrial; L14mt; MRP-L14; 39S ribosomal protein L32, mitochondrial; L32mt; MRP-L32
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDEEPERTKRWEGGYERTWEILKEDESGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRH
LYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTEL
SGNPRKHITSLKEAVDMTCHGEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPS
NIYDLIKTLKAAKIRVSVIGLSAEVRVCTVLARETGGTYHVILDESHYKELLTHHLSPPP
ASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDGNTEPGLTLGGYFCPQCRAKYCE
LPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLEEYNGERFCYGCQGELKDQHVYVC
AVCQNVFCVDCDVFVHDSLHCCPGCIHKIPAPSGV
Target Bioclass
Other
Family
Universal ribosomal protein uL14 family
Subcellular location
Mitochondrion
Function Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation (Probable).
Uniprot ID
Q6P1L8
Ensemble ID
ENST00000372014.5
HGNC ID
HGNC:14279

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH216
 Probe Info  		Y51(12.28)  LDD0259  [2]
STPyne
 Probe Info  		K78(4.73)  LDD0277  [3]
BTD
 Probe Info  		C90(2.40)  LDD2119  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
DBIA
 Probe Info  		C57(9.65)  LDD0209  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [8]
IA-alkyne
 Probe Info  		C90(0.00); C57(0.00)  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		C90(0.00); C57(0.00)  LDD0037  [8]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [9]
TFBX
 Probe Info  		N.A.  LDD0027  [9]
IPM
 Probe Info  		N.A.  LDD0005  [10]
SF
 Probe Info  		N.A.  LDD0028  [11]
Phosphinate-6
 Probe Info  		C90(0.00); C57(0.00)  LDD0018  [12]
Acrolein
 Probe Info  		N.A.  LDD0217  [13]
Crotonaldehyde
 Probe Info  		C90(0.00); H52(0.00)  LDD0219  [13]
AOyne
 Probe Info  		15.00  LDD0443  [14]
NAIA_5
 Probe Info  		C90(0.00); C57(0.00)  LDD2223  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 12.37  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [13]
 LDCM0632  CL-Sc Hep-G2 C90(1.26); C57(1.21)  LDD2227  [15]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C57(1.65)  LDD1702  [4]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0625  F8 Ramos C57(6.60); C90(1.18)  LDD2187  [16]
 LDCM0572  Fragment10 Ramos C57(0.59); C90(1.20)  LDD2189  [16]
 LDCM0573  Fragment11 Ramos C90(0.42)  LDD2190  [16]
 LDCM0574  Fragment12 Ramos C57(0.59); C90(1.00)  LDD2191  [16]
 LDCM0575  Fragment13 Ramos C57(0.52); C90(0.98)  LDD2192  [16]
 LDCM0576  Fragment14 Ramos C57(1.03); C90(0.88)  LDD2193  [16]
 LDCM0579  Fragment20 Ramos C90(1.27)  LDD2194  [16]
 LDCM0580  Fragment21 Ramos C57(0.67); C90(0.87)  LDD2195  [16]
 LDCM0582  Fragment23 Ramos C57(1.94); C90(0.80)  LDD2196  [16]
 LDCM0578  Fragment27 Ramos C57(1.24); C90(0.83)  LDD2197  [16]
 LDCM0586  Fragment28 Ramos C57(0.54); C90(0.73)  LDD2198  [16]
 LDCM0588  Fragment30 Ramos C57(0.98); C90(1.00)  LDD2199  [16]
 LDCM0589  Fragment31 Ramos C90(0.77)  LDD2200  [16]
 LDCM0590  Fragment32 Ramos C57(0.88); C90(1.14)  LDD2201  [16]
 LDCM0468  Fragment33 Ramos C57(1.25); C90(1.02)  LDD2202  [16]
 LDCM0596  Fragment38 Ramos C90(0.76)  LDD2203  [16]
 LDCM0566  Fragment4 Ramos C57(0.88); C90(1.13)  LDD2184  [16]
 LDCM0610  Fragment52 Ramos C90(1.11)  LDD2204  [16]
 LDCM0614  Fragment56 Ramos C57(0.56); C90(0.85)  LDD2205  [16]
 LDCM0569  Fragment7 Ramos C57(0.85); C90(1.16)  LDD2186  [16]
 LDCM0571  Fragment9 Ramos C57(0.48); C90(1.18)  LDD2188  [16]
 LDCM0022  KB02 Ramos C57(1.04); C90(1.73)  LDD2182  [16]
 LDCM0023  KB03 Jurkat C57(9.65)  LDD0209  [6]
 LDCM0024  KB05 G361 C90(3.02)  LDD3311  [17]
 LDCM0109  NEM HeLa N.A.  LDD0223  [13]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C90(2.40)  LDD2119  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C90(1.37)  LDD2127  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C90(0.87)  LDD2135  [4]
 LDCM0627  NUDT7-COV-1 HEK-293T C90(1.03)  LDD2206  [18]
 LDCM0628  OTUB2-COV-1 HEK-293T C90(0.55)  LDD2207  [18]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
17 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
18 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.