Details of the Target

General Information of Target

Target ID LDTP13535
Target Name Palmitoyltransferase ZDHHC8 (ZDHHC8)
Gene Name ZDHHC8
Gene ID 29801
Synonyms
KIAA1292; ZDHHCL1; ZNF378; Palmitoyltransferase ZDHHC8; EC 2.3.1.225; Zinc finger DHHC domain-containing protein 8; DHHC-8; Zinc finger protein 378
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEVFMVYNRTRVK
EPIGKARWPLDTDADMVVSVGTASKELKDFKVRVSYFGEQEDQALGRSVLYLTGVDISLE
VDTGRTGKVKRSQGDKKTWRWGPEGYGAILLVNCDRDNHRSAEPDLTHSWLMSLADLQDM
SPMLLSCNGPDKLFDSHKLVLNVPFSDSKRVRVFCARGGNSLSDYKQVLGPQCLSYEVER
QPGEQEIKFYVEGLTFPDADFLGLVSLSVSLVDPGTLPEVTLFTDTVGFRMAPWIMTPNT
QPPEELYVCRVMDTHGSNEKFLEDMSYLTLKANCKLTICPQVENRNDRWIQDEMEFGYIE
APHKSFPVVFDSPRNRGLKDFPYKRILGPDFGYVTREIPLPGPSSLDSFGNLDVSPPVTV
GGTEYPLGRILIGSSFPKSGGRQMARAVRNFLKAQQVQAPVELYSDWLSVGHVDEFLTFV
PTSDQKGFRLLLASPSACLKLFQEKKEEGYGEAAQFDGLKHQAKRSINEMLADRHLQRDN
LHAQKCIDWNRNVLKRELGLAESDIVDIPQLFFLKNFYAEAFFPDMVNMVVLGKYLGIPK
PYGPIINGRCCLEEKVQSLLEPLGLHCIFIDDYLSYHELQGEIHCGTNVRRKPFPFKWWN
MVP
Target Bioclass
Enzyme
Family
DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily
Subcellular location
Golgi apparatus membrane
Function
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and therefore functions in several unrelated biological processes (Probable). Through the palmitoylation of ABCA1 regulates the localization of the transporter to the plasma membrane and thereby regulates its function in cholesterol and phospholipid efflux (Probable). Could also pamitoylate the D(2) dopamine receptor DRD2 and regulate its stability and localization to the plasma membrane (Probable). Could also play a role in glutamatergic transmission.; (Microbial infection) Able to palmitoylate SARS coronavirus-2/SARS-CoV-2 spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell.
Uniprot ID
Q9ULC8
Ensemble ID
ENST00000320602.11
HGNC ID
HGNC:18474

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.E273G .
CAL33 SNV: p.D516A .
COLO792 SNV: p.R558G .
HCT116 SNV: p.R113H .
HCT15 SNV: p.A65V; p.A188V; p.L435I .
HT115 SNV: p.R144Q .
MELJUSO SNV: p.E560K .
SUPT1 SNV: p.C106Y .
SW1271 SNV: p.S391N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K751(4.84)  LDD0277  [1]
DBIA
 Probe Info  		C370(1.13)  LDD1512  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C370(1.13)  LDD1512  [2]
 LDCM0282  AC22 HEK-293T C370(0.78)  LDD1521  [2]
 LDCM0284  AC24 HEK-293T C370(0.88)  LDD1523  [2]
 LDCM0291  AC30 HEK-293T C370(1.13)  LDD1530  [2]
 LDCM0293  AC32 HEK-293T C370(1.05)  LDD1532  [2]
 LDCM0299  AC38 HEK-293T C370(1.06)  LDD1538  [2]
 LDCM0302  AC40 HEK-293T C370(0.92)  LDD1541  [2]
 LDCM0308  AC46 HEK-293T C370(0.82)  LDD1547  [2]
 LDCM0310  AC48 HEK-293T C370(0.98)  LDD1549  [2]
 LDCM0317  AC54 HEK-293T C370(0.95)  LDD1556  [2]
 LDCM0319  AC56 HEK-293T C370(1.03)  LDD1558  [2]
 LDCM0323  AC6 HEK-293T C370(0.74)  LDD1562  [2]
 LDCM0326  AC62 HEK-293T C370(0.98)  LDD1565  [2]
 LDCM0328  AC64 HEK-293T C370(1.09)  LDD1567  [2]
 LDCM0345  AC8 HEK-293T C370(1.14)  LDD1569  [2]
 LDCM0275  AKOS034007705 HEK-293T C370(1.28)  LDD1514  [2]
 LDCM0368  CL10 HEK-293T C370(0.55)  LDD1572  [2]
 LDCM0372  CL103 HEK-293T C370(0.80)  LDD1576  [2]
 LDCM0376  CL107 HEK-293T C370(0.75)  LDD1580  [2]
 LDCM0381  CL111 HEK-293T C370(0.90)  LDD1585  [2]
 LDCM0385  CL115 HEK-293T C370(0.81)  LDD1589  [2]
 LDCM0389  CL119 HEK-293T C370(0.81)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C370(0.79)  LDD1594  [2]
 LDCM0394  CL123 HEK-293T C370(0.77)  LDD1598  [2]
 LDCM0398  CL127 HEK-293T C370(0.82)  LDD1602  [2]
 LDCM0402  CL15 HEK-293T C370(0.74)  LDD1606  [2]
 LDCM0410  CL22 HEK-293T C370(0.76)  LDD1614  [2]
 LDCM0412  CL24 HEK-293T C370(0.92)  LDD1616  [2]
 LDCM0415  CL27 HEK-293T C370(0.71)  LDD1619  [2]
 LDCM0418  CL3 HEK-293T C370(1.06)  LDD1622  [2]
 LDCM0423  CL34 HEK-293T C370(0.71)  LDD1627  [2]
 LDCM0425  CL36 HEK-293T C370(0.75)  LDD1629  [2]
 LDCM0428  CL39 HEK-293T C370(0.96)  LDD1632  [2]
 LDCM0436  CL46 HEK-293T C370(0.74)  LDD1640  [2]
 LDCM0438  CL48 HEK-293T C370(0.92)  LDD1642  [2]
 LDCM0449  CL58 HEK-293T C370(0.77)  LDD1652  [2]
 LDCM0452  CL60 HEK-293T C370(0.86)  LDD1655  [2]
 LDCM0455  CL63 HEK-293T C370(0.76)  LDD1658  [2]
 LDCM0463  CL70 HEK-293T C370(0.91)  LDD1666  [2]
 LDCM0465  CL72 HEK-293T C370(0.75)  LDD1668  [2]
 LDCM0476  CL82 HEK-293T C370(0.90)  LDD1679  [2]
 LDCM0478  CL84 HEK-293T C370(0.76)  LDD1681  [2]
 LDCM0481  CL87 HEK-293T C370(1.17)  LDD1684  [2]
 LDCM0489  CL94 HEK-293T C370(1.00)  LDD1692  [2]
 LDCM0491  CL96 HEK-293T C370(0.72)  LDD1694  [2]
 LDCM0494  CL99 HEK-293T C370(0.69)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C370(0.79)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C370(0.95)  LDD1671  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402