Details of the Target

General Information of Target

Target ID LDTP13010
Target Name Centrosomal protein of 72 kDa (CEP72)
Gene Name CEP72
Gene ID 55722
Synonyms
KIAA1519; Centrosomal protein of 72 kDa; Cep72
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNAPLDGLSVSSSSTGSLGSAAGAGGGGGAGLRLLSANVRQLHQALTALLSEAEREQFTH
CLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATT
PYRQPAWGGPDSAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKE
GLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQ
GRSISPPSGLPQPHGGALRQQEGDRRSTLHLLQGGDEKKVNLVLGDGRSLGLTIRGGAEY
GLGIYITGVDPGSEAEGSGLKVGDQILEVNGRSFLNILHDEAVRLLKSSRHLILTVKDVG
RLPHARTTVDETKWIASSRIRETMANSAGFLGDLTTEGINKPGFYKGPAGSQVTLSSLGN
QTRVLLEEQARHLLNEQEHATMAYYLDEYRGGSVSVEALVMALFKLLNTHAKFSLLSEVR
GTISPQDLERFDHLVLRREIESMKARQPPGPGAGDTYSMVSYSDTGSSTGSHGTSTTVSS
ARNTLDLEETGEAVQGNINALPDVSVDDVRSTSQGLSSFKPLPRPPPLAQGNDLPLGQPR
KLGREDLQPPSSMPSCSGTVFSAPQNRSPPAGTAPTPGTSSAQDLPSSPIYASVSPANPS
SKRPLDAHLALVNQHPIGPFPRVQSPPHLKSPSAEATVAGGCLLPPSPSGHPDQTGTNQH
FVMVEVHRPDSEPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGEAEASAPGRGR
QSVSTKSRSSKELPRNERPTDGANKPPGLLEPTSTLVRVKKSAATLGIAIEGGANTRQPL
PRIVTIQRGGSAHNCGQLKVGHVILEVNGLTLRGKEHREAARIIAEAFKTKDRDYIDFLV
TEFNVML
Target Bioclass
Other
Family
CEP72 family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs). Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2.
Uniprot ID
Q9P209
Ensemble ID
ENST00000264935.6
HGNC ID
HGNC:25547

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.A28D; p.E618D .
LNCaP clone FGC SNV: p.A548T .
MEWO SNV: p.S594F .
NB1 SNV: p.G249R .
NCIH1048 SNV: p.K185E .
NCIH196 SNV: p.D399H .
SKMEL24 Substitution: p.M304V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AHL-Pu-1
 Probe Info  		C353(2.86)  LDD0168  [1]
DBIA
 Probe Info  		C274(27.62)  LDD0209  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
IPM
 Probe Info  		C353(0.00); C179(0.00)  LDD0147  [5]
TFBX
 Probe Info  		C179(0.00); C245(0.00); C565(0.00)  LDD0148  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [6]
Methacrolein
 Probe Info  		C353(0.00); C437(0.00); C565(0.00)  LDD0218  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C353(2.86)  LDD0168  [1]
 LDCM0026  4SU-RNA+native RNA HEK-293T C353(2.25); C274(9.65)  LDD0169  [1]
 LDCM0214  AC1 HEK-293T C179(1.18); C565(1.04)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C179(0.91)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C179(1.08)  LDD1509  [7]
 LDCM0259  AC14 HEK-293T C179(1.05)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C565(0.96)  LDD1513  [7]
 LDCM0276  AC17 HEK-293T C179(0.99); C565(1.00)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C179(1.15)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C179(1.34)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C179(1.01)  LDD1518  [7]
 LDCM0281  AC21 HEK-293T C179(1.05); C565(0.92)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C179(1.11)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C565(0.97)  LDD1522  [7]
 LDCM0285  AC25 HEK-293T C179(1.01); C565(0.93)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C179(1.09)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C179(1.17)  LDD1526  [7]
 LDCM0289  AC29 HEK-293T C179(0.98); C565(0.89)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C179(0.98)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C179(1.23)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C565(0.94)  LDD1531  [7]
 LDCM0294  AC33 HEK-293T C179(1.07); C565(1.06)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C179(1.24)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C179(1.28)  LDD1535  [7]
 LDCM0298  AC37 HEK-293T C179(1.04); C565(1.02)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C179(1.15)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C565(0.94)  LDD1539  [7]
 LDCM0303  AC41 HEK-293T C179(1.25); C565(1.10)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C179(1.09)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C179(1.72)  LDD1544  [7]
 LDCM0307  AC45 HEK-293T C179(1.01); C565(1.09)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C179(1.24)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C565(0.96)  LDD1548  [7]
 LDCM0311  AC49 HEK-293T C179(1.18); C565(0.99)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C179(0.97); C565(0.91)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C179(1.16)  LDD1552  [7]
 LDCM0314  AC51 HEK-293T C179(1.55)  LDD1553  [7]
 LDCM0316  AC53 HEK-293T C179(1.00); C565(0.93)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C179(1.11)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C565(1.04)  LDD1557  [7]
 LDCM0320  AC57 HEK-293T C179(1.18); C565(1.08)  LDD1559  [7]
 LDCM0321  AC58 HEK-293T C179(1.03)  LDD1560  [7]
 LDCM0322  AC59 HEK-293T C179(1.10)  LDD1561  [7]
 LDCM0323  AC6 HEK-293T C179(1.21)  LDD1562  [7]
 LDCM0325  AC61 HEK-293T C179(0.98); C565(0.95)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C179(1.10)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C565(0.96)  LDD1566  [7]
 LDCM0334  AC7 HEK-293T C565(0.92)  LDD1568  [7]
 LDCM0248  AKOS034007472 HEK-293T C179(1.03); C565(1.01)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C179(1.19); C565(0.97)  LDD1570  [7]
 LDCM0367  CL1 HEK-293T C179(0.99)  LDD1571  [7]
 LDCM0368  CL10 HEK-293T C179(0.99)  LDD1572  [7]
 LDCM0370  CL101 HEK-293T C179(1.04)  LDD1574  [7]
 LDCM0371  CL102 HEK-293T C179(1.36)  LDD1575  [7]
 LDCM0372  CL103 HEK-293T C179(0.96); C565(0.97)  LDD1576  [7]
 LDCM0374  CL105 HEK-293T C179(0.98)  LDD1578  [7]
 LDCM0375  CL106 HEK-293T C179(1.03)  LDD1579  [7]
 LDCM0376  CL107 HEK-293T C179(1.08); C565(0.96)  LDD1580  [7]
 LDCM0378  CL109 HEK-293T C179(1.00)  LDD1582  [7]
 LDCM0379  CL11 HEK-293T C565(1.19)  LDD1583  [7]
 LDCM0380  CL110 HEK-293T C179(1.18)  LDD1584  [7]
 LDCM0381  CL111 HEK-293T C179(0.85); C565(1.11)  LDD1585  [7]
 LDCM0383  CL113 HEK-293T C179(1.03)  LDD1587  [7]
 LDCM0384  CL114 HEK-293T C179(1.00)  LDD1588  [7]
 LDCM0385  CL115 HEK-293T C179(1.00); C565(1.01)  LDD1589  [7]
 LDCM0387  CL117 HEK-293T C179(1.07)  LDD1591  [7]
 LDCM0388  CL118 HEK-293T C179(1.27)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C179(1.03); C565(1.10)  LDD1593  [7]
 LDCM0392  CL121 HEK-293T C179(1.05)  LDD1596  [7]
 LDCM0393  CL122 HEK-293T C179(1.13)  LDD1597  [7]
 LDCM0394  CL123 HEK-293T C179(0.98); C565(1.06)  LDD1598  [7]
 LDCM0396  CL125 HEK-293T C179(1.05)  LDD1600  [7]
 LDCM0397  CL126 HEK-293T C179(1.11)  LDD1601  [7]
 LDCM0398  CL127 HEK-293T C179(0.83); C565(1.01)  LDD1602  [7]
 LDCM0400  CL13 HEK-293T C179(1.10)  LDD1604  [7]
 LDCM0401  CL14 HEK-293T C179(1.12)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C179(1.24); C565(1.04)  LDD1606  [7]
 LDCM0404  CL17 HEK-293T C179(0.98); C565(1.08)  LDD1608  [7]
 LDCM0405  CL18 HEK-293T C179(1.33)  LDD1609  [7]
 LDCM0406  CL19 HEK-293T C179(1.50)  LDD1610  [7]
 LDCM0407  CL2 HEK-293T C179(1.45)  LDD1611  [7]
 LDCM0409  CL21 HEK-293T C179(1.13); C565(1.37)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C179(1.24)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C565(1.19)  LDD1615  [7]
 LDCM0413  CL25 HEK-293T C179(0.98)  LDD1617  [7]
 LDCM0414  CL26 HEK-293T C179(1.02)  LDD1618  [7]
 LDCM0415  CL27 HEK-293T C179(0.97); C565(0.97)  LDD1619  [7]
 LDCM0417  CL29 HEK-293T C179(1.01); C565(1.06)  LDD1621  [7]
 LDCM0418  CL3 HEK-293T C179(0.97); C565(1.14)  LDD1622  [7]
 LDCM0419  CL30 HEK-293T C179(1.11)  LDD1623  [7]
 LDCM0420  CL31 HEK-293T C179(1.24)  LDD1624  [7]
 LDCM0422  CL33 HEK-293T C179(1.10); C565(0.93)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C179(1.30)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C565(1.34)  LDD1628  [7]
 LDCM0426  CL37 HEK-293T C179(1.00)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C179(1.08); C565(1.04)  LDD1632  [7]
 LDCM0431  CL41 HEK-293T C179(0.97); C565(1.10)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C179(1.01)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C179(1.09)  LDD1637  [7]
 LDCM0435  CL45 HEK-293T C179(1.15); C565(1.00)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C179(1.36)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C565(1.20)  LDD1641  [7]
 LDCM0439  CL49 HEK-293T C179(1.04)  LDD1643  [7]
 LDCM0440  CL5 HEK-293T C179(1.03); C565(1.11)  LDD1644  [7]
 LDCM0441  CL50 HEK-293T C179(1.37)  LDD1645  [7]
 LDCM0444  CL53 HEK-293T C179(0.81); C565(1.22)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C179(1.11)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C179(1.43)  LDD1649  [7]
 LDCM0448  CL57 HEK-293T C179(1.22); C565(1.29)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C179(1.62)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C565(1.21)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C179(0.86)  LDD1654  [7]
 LDCM0453  CL61 HEK-293T C179(1.05)  LDD1656  [7]
 LDCM0454  CL62 HEK-293T C179(1.21)  LDD1657  [7]
 LDCM0455  CL63 HEK-293T C179(1.01); C565(1.13)  LDD1658  [7]
 LDCM0457  CL65 HEK-293T C179(1.08); C565(0.98)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C179(1.06)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C179(1.03)  LDD1662  [7]
 LDCM0461  CL69 HEK-293T C179(1.16); C565(0.95)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C179(1.19)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C179(1.36)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C565(1.27)  LDD1667  [7]
 LDCM0466  CL73 HEK-293T C179(0.88)  LDD1669  [7]
 LDCM0467  CL74 HEK-293T C179(1.24)  LDD1670  [7]
 LDCM0470  CL77 HEK-293T C179(1.00); C565(1.08)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C179(1.11)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C179(1.07)  LDD1675  [7]
 LDCM0475  CL81 HEK-293T C179(1.08); C565(1.05)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C179(1.24)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C565(1.10)  LDD1680  [7]
 LDCM0479  CL85 HEK-293T C179(0.90)  LDD1682  [7]
 LDCM0480  CL86 HEK-293T C179(0.93)  LDD1683  [7]
 LDCM0481  CL87 HEK-293T C179(0.84); C565(1.02)  LDD1684  [7]
 LDCM0483  CL89 HEK-293T C179(1.05); C565(1.06)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C179(1.05); C565(1.27)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C179(1.28)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C179(1.07)  LDD1689  [7]
 LDCM0488  CL93 HEK-293T C179(1.03); C565(1.05)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C179(1.03)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C565(1.03)  LDD1693  [7]
 LDCM0492  CL97 HEK-293T C179(0.96)  LDD1695  [7]
 LDCM0493  CL98 HEK-293T C179(1.31)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C179(0.81); C565(0.95)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C179(1.10); C565(1.09)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C179(0.97); C565(1.08)  LDD1671  [7]
 LDCM0427  Fragment51 HEK-293T C179(1.12)  LDD1631  [7]
 LDCM0022  KB02 A2780 C565(1.70)  LDD2254  [8]
 LDCM0023  KB03 Jurkat C274(27.62)  LDD0209  [2]
 LDCM0024  KB05 NCI-H3122 C565(2.08)  LDD3360  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
AMSH-like protease (STAMBPL1) Peptidase M67C family Q96FJ0
Proteasome subunit alpha type-1 (PSMA1) Peptidase T1A family P25786
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (PIN4) PpiC/parvulin rotamase family Q9Y237
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pericentriolar material 1 protein (PCM1) PCM1 family Q15154
Optineurin (OPTN) . Q96CV9
Other
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-7 (ATXN7) Ataxin-7 family O15265
Ciliogenesis-associated TTC17-interacting protein (CATIP) CATIP family Q7Z7H3
Protein FAM161A (FAM161A) FAM161 family Q3B820
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
RNA-binding protein FXR1 (FXR1) FMR1 family P51114
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1
Protein INCA1 (INCA1) INCA family Q0VD86
Centrosomal protein kizuna (KIZ) Kizuna family Q2M2Z5
MICOS complex subunit MIC19 (CHCHD3) MICOS complex subunit Mic19 family Q9NX63
Spermatogenesis-associated protein 24 (SPATA24) SPATA24 family Q86W54
CDK5 regulatory subunit-associated protein 2 (CDK5RAP2) . Q96SN8
Sperm-associated antigen 5 (SPAG5) . Q96R06

References

1 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840