Details of the Target

General Information of Target

Target ID LDTP12525
Target Name rRNA N6-adenosine-methyltransferase METTL5 (METTL5)
Gene Name METTL5
Gene ID 29081
Synonyms
rRNA N6-adenosine-methyltransferase METTL5; EC 2.1.1.-; Methyltransferase-like protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVFPAKRFCLVPSMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAA
MAGRLMIRKLVAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAA
EPELQVGIDIMKTSFPGRGSIPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALK
ESFIKAIGVGLGFELQRLEFDLSPLNLDIGQVYKETRLFLDGEEEKEWAFEESKIDEHHF
VAVALRKPDGSRHQDVPSQDDSKPTQRQFTILNFNDLMSSAVPMTPEDPSFWDCFCFTEE
IPIRNGTKS
Target Bioclass
Enzyme
Family
Methyltransferase superfamily, PrmA family
Subcellular location
Nucleus
Function
Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation.
Uniprot ID
Q9NRN9
Ensemble ID
ENST00000260953.10
HGNC ID
HGNC:25006

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K143(0.53); K158(3.44)  LDD0277  [1]
m-APA
 Probe Info  		15.00  LDD0403  [2]
HHS-475
 Probe Info  		Y41(0.77)  LDD0264  [3]
DBIA
 Probe Info  		C38(0.99)  LDD1492  [4]
ATP probe
 Probe Info  		K21(0.00); K23(0.00); K132(0.00); K135(0.00)  LDD0199  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
HHS-465
 Probe Info  		K53(0.00); Y41(0.00)  LDD2240  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C110(0.84)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C110(1.20)  LDD1509  [4]
 LDCM0259  AC14 HEK-293T C38(0.95); C110(1.05)  LDD1512  [4]
 LDCM0277  AC18 HEK-293T C110(1.01)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C110(0.87)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C110(0.90)  LDD1518  [4]
 LDCM0281  AC21 HEK-293T C110(0.85)  LDD1520  [4]
 LDCM0282  AC22 HEK-293T C38(1.00); C110(1.04)  LDD1521  [4]
 LDCM0284  AC24 HEK-293T C110(1.25)  LDD1523  [4]
 LDCM0286  AC26 HEK-293T C110(0.93)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C110(1.20)  LDD1526  [4]
 LDCM0289  AC29 HEK-293T C110(0.70)  LDD1528  [4]
 LDCM0290  AC3 HEK-293T C110(1.01)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C38(0.99); C110(1.07)  LDD1530  [4]
 LDCM0293  AC32 HEK-293T C110(1.17)  LDD1532  [4]
 LDCM0295  AC34 HEK-293T C110(0.81)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C110(1.45)  LDD1535  [4]
 LDCM0298  AC37 HEK-293T C110(1.15)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C38(0.90); C110(0.99)  LDD1538  [4]
 LDCM0302  AC40 HEK-293T C110(1.24)  LDD1541  [4]
 LDCM0304  AC42 HEK-293T C110(0.99)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C110(1.14)  LDD1544  [4]
 LDCM0307  AC45 HEK-293T C110(0.93)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C38(1.10); C110(1.02)  LDD1547  [4]
 LDCM0310  AC48 HEK-293T C110(1.16)  LDD1549  [4]
 LDCM0312  AC5 HEK-293T C110(0.68)  LDD1551  [4]
 LDCM0313  AC50 HEK-293T C110(0.91)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C110(1.10)  LDD1553  [4]
 LDCM0316  AC53 HEK-293T C110(0.89)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C38(1.01); C110(0.96)  LDD1556  [4]
 LDCM0319  AC56 HEK-293T C110(1.20)  LDD1558  [4]
 LDCM0321  AC58 HEK-293T C110(0.84)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C110(1.21)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C38(0.97); C110(1.00)  LDD1562  [4]
 LDCM0325  AC61 HEK-293T C110(0.91)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C38(0.96); C110(0.93)  LDD1565  [4]
 LDCM0328  AC64 HEK-293T C110(1.17)  LDD1567  [4]
 LDCM0345  AC8 HEK-293T C110(1.17)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C110(0.96)  LDD1511  [4]
 LDCM0275  AKOS034007705 HEK-293T C110(1.20)  LDD1514  [4]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [2]
 LDCM0367  CL1 HEK-293T C110(1.15)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C38(0.72); C110(0.97)  LDD1572  [4]
 LDCM0370  CL101 HEK-293T C110(0.91)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C110(0.81)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C38(0.99); C110(1.21)  LDD1576  [4]
 LDCM0374  CL105 HEK-293T C110(0.91)  LDD1578  [4]
 LDCM0375  CL106 HEK-293T C110(1.00)  LDD1579  [4]
 LDCM0376  CL107 HEK-293T C38(0.88); C110(1.17)  LDD1580  [4]
 LDCM0378  CL109 HEK-293T C110(1.01)  LDD1582  [4]
 LDCM0380  CL110 HEK-293T C110(0.81)  LDD1584  [4]
 LDCM0381  CL111 HEK-293T C38(0.92); C110(1.14)  LDD1585  [4]
 LDCM0383  CL113 HEK-293T C110(0.95)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C110(0.62)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C38(0.96); C110(0.94)  LDD1589  [4]
 LDCM0387  CL117 HEK-293T C110(1.19)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C110(1.02)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C38(1.05); C110(1.05)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C110(0.96)  LDD1594  [4]
 LDCM0392  CL121 HEK-293T C110(0.86)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C110(0.87)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C38(0.88); C110(1.05)  LDD1598  [4]
 LDCM0396  CL125 HEK-293T C110(1.04)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C110(0.79)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C38(0.96); C110(1.17)  LDD1602  [4]
 LDCM0400  CL13 HEK-293T C110(0.85)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C110(0.92)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C38(0.82); C110(1.11)  LDD1606  [4]
 LDCM0405  CL18 HEK-293T C110(0.97)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C110(1.05)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C110(0.76)  LDD1611  [4]
 LDCM0409  CL21 HEK-293T C110(0.79)  LDD1613  [4]
 LDCM0410  CL22 HEK-293T C38(0.66); C110(1.04)  LDD1614  [4]
 LDCM0412  CL24 HEK-293T C110(1.08)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C110(0.70)  LDD1617  [4]
 LDCM0414  CL26 HEK-293T C110(0.85)  LDD1618  [4]
 LDCM0415  CL27 HEK-293T C38(0.91); C110(0.93)  LDD1619  [4]
 LDCM0418  CL3 HEK-293T C38(0.93); C110(0.92)  LDD1622  [4]
 LDCM0419  CL30 HEK-293T C110(0.86)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C110(1.30)  LDD1624  [4]
 LDCM0422  CL33 HEK-293T C110(1.58)  LDD1626  [4]
 LDCM0423  CL34 HEK-293T C38(0.63); C110(1.02)  LDD1627  [4]
 LDCM0425  CL36 HEK-293T C110(1.73)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C110(1.02)  LDD1630  [4]
 LDCM0428  CL39 HEK-293T C38(0.93); C110(1.15)  LDD1632  [4]
 LDCM0432  CL42 HEK-293T C110(0.95)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C110(1.08)  LDD1637  [4]
 LDCM0435  CL45 HEK-293T C110(1.07)  LDD1639  [4]
 LDCM0436  CL46 HEK-293T C38(0.74); C110(1.00)  LDD1640  [4]
 LDCM0438  CL48 HEK-293T C110(1.24)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C110(1.11)  LDD1643  [4]
 LDCM0441  CL50 HEK-293T C110(0.88)  LDD1645  [4]
 LDCM0445  CL54 HEK-293T C110(1.02)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C110(1.13)  LDD1649  [4]
 LDCM0448  CL57 HEK-293T C110(0.86)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C38(0.72); C110(1.04)  LDD1652  [4]
 LDCM0451  CL6 HEK-293T C110(0.83)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C110(1.15)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C110(0.98)  LDD1656  [4]
 LDCM0454  CL62 HEK-293T C110(0.76)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C38(0.85); C110(0.94)  LDD1658  [4]
 LDCM0458  CL66 HEK-293T C110(0.90)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C110(1.17)  LDD1662  [4]
 LDCM0461  CL69 HEK-293T C110(1.00)  LDD1664  [4]
 LDCM0462  CL7 HEK-293T C110(0.99)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C38(0.81); C110(1.02)  LDD1666  [4]
 LDCM0465  CL72 HEK-293T C110(1.12)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C110(1.00)  LDD1669  [4]
 LDCM0467  CL74 HEK-293T C110(0.94)  LDD1670  [4]
 LDCM0471  CL78 HEK-293T C110(1.06)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C110(1.32)  LDD1675  [4]
 LDCM0475  CL81 HEK-293T C110(0.75)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C38(0.65); C110(0.98)  LDD1679  [4]
 LDCM0478  CL84 HEK-293T C110(0.96)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C110(0.98)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C110(0.76)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C38(0.96); C110(1.12)  LDD1684  [4]
 LDCM0484  CL9 HEK-293T C110(0.96)  LDD1687  [4]
 LDCM0485  CL90 HEK-293T C110(1.21)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C110(1.31)  LDD1689  [4]
 LDCM0488  CL93 HEK-293T C110(0.94)  LDD1691  [4]
 LDCM0489  CL94 HEK-293T C38(0.79); C110(0.89)  LDD1692  [4]
 LDCM0491  CL96 HEK-293T C110(1.28)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C110(0.77)  LDD1695  [4]
 LDCM0493  CL98 HEK-293T C110(0.86)  LDD1696  [4]
 LDCM0494  CL99 HEK-293T C38(1.07); C110(0.97)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C38(0.91); C110(1.07)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C38(0.84); C110(1.01)  LDD1671  [4]
 LDCM0427  Fragment51 HEK-293T C110(0.81)  LDD1631  [4]
 LDCM0116  HHS-0101 DM93 Y41(0.77)  LDD0264  [3]
 LDCM0120  HHS-0701 DM93 Y41(2.31)  LDD0268  [3]
 LDCM0022  KB02 HEK-293T C38(0.99)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C38(1.00)  LDD1497  [4]
 LDCM0024  KB05 HEK-293T C38(1.10)  LDD1502  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 5 (ZBTB5) . O15062
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) TRM112 family Q9UI30

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010