Details of the Target

General Information of Target

Target ID LDTP11667
Target Name Twisted gastrulation protein homolog 1 (TWSG1)
Gene Name TWSG1
Gene ID 57045
Synonyms
TSG; Twisted gastrulation protein homolog 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELL
FLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRK
AEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRIL
LPLYEVDDLRDAFRTLGL
Target Bioclass
Other
Family
Twisted gastrulation protein family
Subcellular location
Secreted
Function
May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development.
Uniprot ID
Q9GZX9
Ensemble ID
ENST00000262120.10
HGNC ID
HGNC:12429

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
LS180 SNV: p.C56F .
TC71 SNV: p.Ter224QextTer10 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C31(2.58)  LDD3342  [1]
BTD
 Probe Info  		C31(0.80)  LDD2113  [2]
IPM
 Probe Info  		C53(1.10)  LDD1701  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
IA-alkyne
 Probe Info  		C31(0.00); C185(0.00)  LDD0165  [4]
AOyne
 Probe Info  		12.20  LDD0443  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C31(0.87)  LDD1523  [6]
 LDCM0293  AC32 HEK-293T C31(1.65)  LDD1532  [6]
 LDCM0302  AC40 HEK-293T C31(1.19)  LDD1541  [6]
 LDCM0310  AC48 HEK-293T C31(1.20)  LDD1549  [6]
 LDCM0319  AC56 HEK-293T C31(1.10)  LDD1558  [6]
 LDCM0328  AC64 HEK-293T C31(1.10)  LDD1567  [6]
 LDCM0345  AC8 HEK-293T C31(0.89)  LDD1569  [6]
 LDCM0520  AKOS000195272 MDA-MB-231 C31(0.80)  LDD2113  [2]
 LDCM0275  AKOS034007705 HEK-293T C31(0.82)  LDD1514  [6]
 LDCM0369  CL100 HEK-293T C185(0.89)  LDD1573  [6]
 LDCM0373  CL104 HEK-293T C185(1.03)  LDD1577  [6]
 LDCM0377  CL108 HEK-293T C185(0.95)  LDD1581  [6]
 LDCM0382  CL112 HEK-293T C185(0.91)  LDD1586  [6]
 LDCM0386  CL116 HEK-293T C185(1.02)  LDD1590  [6]
 LDCM0390  CL12 HEK-293T C31(0.89)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C185(0.95)  LDD1595  [6]
 LDCM0395  CL124 HEK-293T C185(0.91)  LDD1599  [6]
 LDCM0399  CL128 HEK-293T C185(1.02)  LDD1603  [6]
 LDCM0403  CL16 HEK-293T C185(1.03)  LDD1607  [6]
 LDCM0412  CL24 HEK-293T C31(1.05)  LDD1616  [6]
 LDCM0416  CL28 HEK-293T C185(0.96)  LDD1620  [6]
 LDCM0425  CL36 HEK-293T C31(1.25)  LDD1629  [6]
 LDCM0429  CL4 HEK-293T C185(0.98)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C185(1.02)  LDD1634  [6]
 LDCM0438  CL48 HEK-293T C31(1.06)  LDD1642  [6]
 LDCM0443  CL52 HEK-293T C185(1.05)  LDD1646  [6]
 LDCM0452  CL60 HEK-293T C31(1.01)  LDD1655  [6]
 LDCM0456  CL64 HEK-293T C185(0.96)  LDD1659  [6]
 LDCM0465  CL72 HEK-293T C31(1.30)  LDD1668  [6]
 LDCM0469  CL76 HEK-293T C185(1.01)  LDD1672  [6]
 LDCM0478  CL84 HEK-293T C31(1.02)  LDD1681  [6]
 LDCM0482  CL88 HEK-293T C185(1.03)  LDD1685  [6]
 LDCM0491  CL96 HEK-293T C31(0.97)  LDD1694  [6]
 LDCM0022  KB02 A2780 C31(1.38)  LDD2254  [1]
 LDCM0023  KB03 MDA-MB-231 C53(1.10)  LDD1701  [2]
 LDCM0024  KB05 NCI-H1155 C31(2.58)  LDD3342  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gap junction alpha-8 protein (GJA8) Connexin family P48165
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G-protein coupled receptor family C group 5 member D (GPRC5D) G-protein coupled receptor 3 family Q9NZD1
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leucine-rich repeat-containing protein 4C (LRRC4C) . Q9HCJ2
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) SGT family O43765
Fibronectin type III domain-containing protein 9 (FNDC9) . Q8TBE3

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402