Details of the Target

General Information of Target

Target ID LDTP10946
Target Name Lysosomal-trafficking regulator (LYST)
Gene Name LYST
Gene ID 1130
Synonyms
CHS; CHS1; Lysosomal-trafficking regulator; Beige homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
METNCRKLVSACVQLGVQPAAVECLFSKDSEIKKVEFTDSPESRKEAASSKFFPRQHPGA
NEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAV
WTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWA
AKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLN
SLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQ
NPASNLSACQYAVDRPV
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes. Might regulate trafficking of effectors involved in exocytosis. In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules. In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes. In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways.
Uniprot ID
Q99698
Ensemble ID
ENST00000389793.7
HGNC ID
HGNC:1968

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.I3338N .
BICR22 SNV: p.H3615Q .
CCK81 SNV: p.Y1711H .
COLO320 SNV: p.R329Ter .
COLO792 Substitution: p.P3458L DBIA    Probe Info 
DOTC24510 SNV: p.G43E .
DU145 SNV: p.M2327V .
EFO27 SNV: p.A3399V .
GAMG SNV: p.M2742T .
HCC15 SNV: p.C3761F .
HCC1937 SNV: p.I1838V .
HCT116 SNV: p.V3228M; p.Q3442Ter .
HCT15 SNV: p.V1705F; p.A3130T .
HELA SNV: p.D1236H .
HT115 SNV: p.S279F; p.L327F; p.K1317N; p.A2833E .
Ishikawa (Heraklio) 02 ER SNV: p.L1418F .
JURKAT SNV: p.V2552I; p.E3454K .
K562 SNV: p.L3513I .
KPL1 SNV: p.L2438Q .
LNCaP clone FGC SNV: p.S60Y .
LS180 SNV: p.A3800V .
MCC13 SNV: p.S1680L; p.C2965W .
MCF7 SNV: p.L2438Q .
MDAMB453 SNV: p.G314C; p.E651Q .
MEWO SNV: p.D1175N; p.E2901K; p.F3001S DBIA    Probe Info 
MFE296 SNV: p.V761A .
MFE319 Insertion: p.L1297FfsTer5 .
MIAPACA2 SNV: p.L3345V .
MM1S SNV: p.M966I .
MV411 SNV: p.A1284T DBIA    Probe Info 
NALM6 SNV: p.R145Q .
NCIH2286 SNV: p.D2412Y .
NCIH358 SNV: p.G1810A .
NCIH446 SNV: p.R503L .
PF382 SNV: p.M452T .
RD SNV: p.A1290S .
RL952 SNV: p.I639V .
SBC5 SNV: p.P3334L .
SKMEL2 SNV: p.V1346I .
SNGM SNV: p.Q2184H .
SNU1 SNV: p.Y1753C DBIA    Probe Info 
SNU5 SNV: p.P3120H .
SUIT2 SNV: p.E848A .
TFK1 SNV: p.A2935T .
WM2664 Insertion: p.L1725PfsTer15 DBIA    Probe Info 
YSCCC SNV: p.T641I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C3505(2.26)  LDD3325  [1]
IA-alkyne
 Probe Info  		C2127(6.69)  LDD1706  [2]
Acrolein
 Probe Info  		C1113(0.00); H1115(0.00)  LDD0221  [3]
CY-1
 Probe Info  		Q115(0.00); Q119(0.00)  LDD0246  [4]
IPM
 Probe Info  		N.A.  LDD2156  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C1827(1.02)  LDD1507  [6]
 LDCM0215  AC10 HEK-293T C2780(1.07); C2127(0.96)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C1827(1.33)  LDD1509  [6]
 LDCM0259  AC14 HEK-293T C1827(1.02)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C1827(1.05)  LDD1513  [6]
 LDCM0276  AC17 HEK-293T C1827(0.86)  LDD1515  [6]
 LDCM0277  AC18 HEK-293T C2780(1.04); C2127(1.21)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C1827(1.40)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C2780(1.10); C2127(1.28)  LDD1518  [6]
 LDCM0281  AC21 HEK-293T C3649(0.95)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C1827(0.91)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C1827(0.89)  LDD1522  [6]
 LDCM0285  AC25 HEK-293T C1827(0.97)  LDD1524  [6]
 LDCM0286  AC26 HEK-293T C2780(1.07); C2127(1.09)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C1827(1.44)  LDD1526  [6]
 LDCM0289  AC29 HEK-293T C3649(1.00)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C1827(1.18)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C1827(1.25)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C1827(0.83)  LDD1531  [6]
 LDCM0294  AC33 HEK-293T C1827(0.89)  LDD1533  [6]
 LDCM0295  AC34 HEK-293T C2780(1.05); C2127(1.10)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C1827(1.15)  LDD1535  [6]
 LDCM0298  AC37 HEK-293T C3649(1.08)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C1827(1.18)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C1827(1.01)  LDD1539  [6]
 LDCM0303  AC41 HEK-293T C1827(1.07)  LDD1542  [6]
 LDCM0304  AC42 HEK-293T C2780(1.00); C2127(1.23)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C1827(1.40)  LDD1544  [6]
 LDCM0307  AC45 HEK-293T C3649(1.08)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C1827(1.10)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C1827(1.14)  LDD1548  [6]
 LDCM0311  AC49 HEK-293T C1827(0.95)  LDD1550  [6]
 LDCM0312  AC5 HEK-293T C3649(0.94)  LDD1551  [6]
 LDCM0313  AC50 HEK-293T C2780(1.04); C2127(1.16)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C1827(1.16)  LDD1553  [6]
 LDCM0316  AC53 HEK-293T C3649(0.90)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C1827(1.09)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C1827(0.84)  LDD1557  [6]
 LDCM0320  AC57 HEK-293T C1827(1.08)  LDD1559  [6]
 LDCM0321  AC58 HEK-293T C2780(1.06); C2127(1.08)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C1827(1.14)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C1827(1.05)  LDD1562  [6]
 LDCM0325  AC61 HEK-293T C3649(0.87)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C1827(1.49)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C1827(1.01)  LDD1566  [6]
 LDCM0334  AC7 HEK-293T C1827(0.85)  LDD1568  [6]
 LDCM0248  AKOS034007472 HEK-293T C3649(0.93)  LDD1511  [6]
 LDCM0356  AKOS034007680 HEK-293T C1827(0.99)  LDD1570  [6]
 LDCM0367  CL1 HEK-293T C1827(0.82); C2780(1.02)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C1827(1.82)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C1827(0.81); C2780(0.97)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C1827(0.98); C2780(0.95)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C1827(1.36); C1337(1.01)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C1827(1.11)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C1827(0.69); C2780(0.96)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C1827(0.81); C2780(0.71)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C1827(1.23); C1337(0.89)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C1827(1.00)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C1827(0.91); C2780(1.04)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C1827(0.97); C2780(0.72)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C1827(1.44)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C1827(1.72); C1337(0.23)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C1827(1.04)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C1827(0.98); C2780(1.02)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C1827(0.91); C2780(0.83)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C1827(1.20); C1337(0.73)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C1827(1.11)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C1827(1.28); C2780(0.92)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C1827(1.02); C2780(0.91)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C1827(1.24); C1337(0.75)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C1827(1.13)  LDD1593  [6]
 LDCM0391  CL120 HEK-293T C1827(0.76); C2780(0.93)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C1827(0.97); C2780(0.81)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C1827(1.54); C1337(0.78)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C1827(1.14)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C1827(0.73); C2780(1.06)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C1827(1.08); C2780(0.86)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C1827(1.54); C1337(0.96)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C1827(1.07)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C1827(0.88); C2780(0.98)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C1827(1.21); C2780(0.87)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C1827(1.18); C1337(0.85)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C1827(0.94)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C1827(0.93); C2780(1.06)  LDD1607  [6]
 LDCM0404  CL17 HEK-293T C1827(1.02)  LDD1608  [6]
 LDCM0405  CL18 HEK-293T C2780(1.09); C2127(1.07)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C1827(1.37)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C1827(1.59); C1337(1.00)  LDD1611  [6]
 LDCM0409  CL21 HEK-293T C3649(1.24)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C1827(1.48)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C1827(1.34)  LDD1615  [6]
 LDCM0413  CL25 HEK-293T C1827(0.85); C2780(0.81)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C1827(1.03); C1337(0.69)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C1827(1.17)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C1827(0.82); C2780(0.87)  LDD1620  [6]
 LDCM0417  CL29 HEK-293T C1827(1.19)  LDD1621  [6]
 LDCM0418  CL3 HEK-293T C1827(1.03)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C2780(1.02); C2127(0.93)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C1827(1.51)  LDD1624  [6]
 LDCM0422  CL33 HEK-293T C3649(1.16)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C1827(1.46)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C1827(1.32)  LDD1628  [6]
 LDCM0426  CL37 HEK-293T C1827(0.96); C2780(0.94)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C1827(0.98)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C1827(1.04); C2780(1.34)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C1827(0.76); C2780(0.94)  LDD1634  [6]
 LDCM0431  CL41 HEK-293T C1827(0.86)  LDD1635  [6]
 LDCM0432  CL42 HEK-293T C2780(1.13); C2127(1.15)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C1827(1.87)  LDD1637  [6]
 LDCM0435  CL45 HEK-293T C3649(0.99)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C1827(1.36)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C1827(1.48)  LDD1641  [6]
 LDCM0439  CL49 HEK-293T C1827(0.94); C2780(0.80)  LDD1643  [6]
 LDCM0440  CL5 HEK-293T C1827(1.27)  LDD1644  [6]
 LDCM0441  CL50 HEK-293T C1827(1.26); C1337(0.91)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C1827(0.90); C2780(0.92)  LDD1646  [6]
 LDCM0444  CL53 HEK-293T C1827(1.04)  LDD1647  [6]
 LDCM0445  CL54 HEK-293T C2780(1.37); C2127(1.09)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C1827(1.55)  LDD1649  [6]
 LDCM0448  CL57 HEK-293T C3649(1.28)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C1827(1.50)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C1827(1.12)  LDD1653  [6]
 LDCM0451  CL6 HEK-293T C2780(1.40); C2127(1.32)  LDD1654  [6]
 LDCM0453  CL61 HEK-293T C1827(1.15); C2780(1.08)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C1827(1.40); C1337(0.87)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C1827(0.97)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C1827(1.11); C2780(1.04)  LDD1659  [6]
 LDCM0457  CL65 HEK-293T C1827(1.14)  LDD1660  [6]
 LDCM0458  CL66 HEK-293T C2780(1.12); C2127(0.97)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C1827(1.43)  LDD1662  [6]
 LDCM0461  CL69 HEK-293T C3649(1.07)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C1827(1.73)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C1827(1.42)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C1827(1.47)  LDD1667  [6]
 LDCM0466  CL73 HEK-293T C1827(0.98); C2780(1.15)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C1827(1.40); C1337(0.69)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C1827(1.06); C2780(0.92)  LDD1672  [6]
 LDCM0470  CL77 HEK-293T C1827(1.04)  LDD1673  [6]
 LDCM0471  CL78 HEK-293T C2780(1.03); C2127(1.03)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C1827(1.55)  LDD1675  [6]
 LDCM0475  CL81 HEK-293T C3649(0.92)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C1827(1.30)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C1827(1.18)  LDD1680  [6]
 LDCM0479  CL85 HEK-293T C1827(0.92); C2780(0.94)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C1827(1.73); C1337(0.80)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C1827(1.21)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C1827(0.86); C2780(0.97)  LDD1685  [6]
 LDCM0483  CL89 HEK-293T C1827(0.97)  LDD1686  [6]
 LDCM0484  CL9 HEK-293T C3649(1.14)  LDD1687  [6]
 LDCM0485  CL90 HEK-293T C2780(1.40); C2127(1.18)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C1827(1.66)  LDD1689  [6]
 LDCM0488  CL93 HEK-293T C3649(1.08)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C1827(1.07)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C1827(1.15)  LDD1693  [6]
 LDCM0492  CL97 HEK-293T C1827(0.88); C2780(1.01)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C1827(1.26); C1337(0.85)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C1827(1.08)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C1827(1.00)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C1827(1.18)  LDD1671  [6]
 LDCM0427  Fragment51 HEK-293T C1827(1.22); C1337(0.80)  LDD1631  [6]
 LDCM0107  IAA HeLa C1113(0.00); H1115(0.00)  LDD0221  [3]
 LDCM0022  KB02 HEK-293T C1337(0.94)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C1337(0.90)  LDD1497  [6]
 LDCM0024  KB05 UACC257 C3505(2.26)  LDD3325  [1]
 LDCM0109  NEM HeLa C1113(0.00); H1115(0.00)  LDD0225  [3]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402