Details of the Target

General Information of Target

Target ID LDTP10611
Target Name PWWP domain-containing protein 2A (PWWP2A)
Gene Name PWWP2A
Gene ID 114825
Synonyms
KIAA1935; MST101; PWWP domain-containing protein 2A
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MLHEEAAQKRKGKEPGMALPQGRLTFRDVAIEFSLAEWKFLNPAQRALYREVMLENYRNL
EAVDISSKRMMKEVLSTGQGNTEVIHTGMLQRHESYHTGDFCFQEIEKDIHDFEFQSQKD
ERNGHEASMPKIKELMGSTDRHDQRHAGNKPIKDQLGLSFHLHLPELHIFQPEEKIANQV
EKSVNDASSISTSQRISCRPETHTPNNYGNNFFHSSLLTQKQEVHMREKSFQCNETGEAF
NCSSFVRKHQIIHLGEKQYKFDICGKVFNEKRYLARHRRCHTSEKPYKCNECGKSFSYKS
SLTCHRRCHTGEKPYKCNECGKSFSYKSSLTCHHRCHTGEKPYKCNECGKSFSYKSSLRC
HRRLHTGIKPYKCNECGKMFGQNSTLVIHKAIHTGEKPYKCNECGKAFNQQSHLSRHHRL
HTGEKPYKCNDCGKAFIHQSSLARHHRLHTGEKSYKCEECDRVFSQKSNLERHKIIHTGE
KPYKCNECHKTFSHRSSLPCHRRLHSGEKPYKCNECGKTFNVQSHLSRHHRLHTGEKPYK
CKVCDKAFMCHSYLANHTRIHSGEKPYKCNECGKAHNHLIDSSIKPCMSS
Target Bioclass
Enzyme
Subcellular location
Nucleus
Function
Chromatin-binding protein that acts as an adapter between distinct nucleosome components (H3K36me3 or H2A.Z) and chromatin-modifying complexes, contributing to the regulation of the levels of histone acetylation at actively transcribed genes. Competes with CHD4 and MBD3 for interaction with MTA1 to form a NuRD subcomplex, preventing the formation of full NuRD complex (containing CHD4 and MBD3), leading to recruitment of HDACs to gene promoters resulting in turn in the deacetylation of nearby H3K27 and H2A.Z. Plays a role in facilitating transcriptional elongation and repression of spurious transcription initiation through regulation of histone acetylation. Essential for proper mitosis progression.
Uniprot ID
Q96N64
Ensemble ID
ENST00000307063.9
HGNC ID
HGNC:29406

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.G493R .
HT115 SNV: p.S679N .
KASUMI1 SNV: p.V153G .
LS180 SNV: p.F277L .
NCIH1155 SNV: p.A104P .
NCIH1993 SNV: p.A104P .
RKO Deletion: p.V417FfsTer16 .
SW756 SNV: p.G187W .
TCCSUP SNV: p.Y348C .
TOV21G SNV: p.G149W .
WM2664 Insertion: p.N327KfsTer6 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C654(1.98)  LDD3339  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0167  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C233(1.50)  LDD1513  [3]
 LDCM0281  AC21 HEK-293T C233(1.29)  LDD1520  [3]
 LDCM0283  AC23 HEK-293T C233(1.21)  LDD1522  [3]
 LDCM0284  AC24 HEK-293T C233(0.87)  LDD1523  [3]
 LDCM0289  AC29 HEK-293T C233(0.97)  LDD1528  [3]
 LDCM0292  AC31 HEK-293T C233(0.99)  LDD1531  [3]
 LDCM0293  AC32 HEK-293T C233(1.04)  LDD1532  [3]
 LDCM0298  AC37 HEK-293T C233(0.84)  LDD1537  [3]
 LDCM0300  AC39 HEK-293T C233(1.00)  LDD1539  [3]
 LDCM0302  AC40 HEK-293T C233(1.02)  LDD1541  [3]
 LDCM0307  AC45 HEK-293T C233(1.16)  LDD1546  [3]
 LDCM0309  AC47 HEK-293T C233(1.13)  LDD1548  [3]
 LDCM0310  AC48 HEK-293T C233(1.02)  LDD1549  [3]
 LDCM0312  AC5 HEK-293T C233(1.13)  LDD1551  [3]
 LDCM0316  AC53 HEK-293T C233(1.05)  LDD1555  [3]
 LDCM0318  AC55 HEK-293T C233(0.86)  LDD1557  [3]
 LDCM0319  AC56 HEK-293T C233(0.87)  LDD1558  [3]
 LDCM0325  AC61 HEK-293T C233(1.05)  LDD1564  [3]
 LDCM0327  AC63 HEK-293T C233(1.06)  LDD1566  [3]
 LDCM0328  AC64 HEK-293T C233(0.90)  LDD1567  [3]
 LDCM0334  AC7 HEK-293T C233(1.01)  LDD1568  [3]
 LDCM0345  AC8 HEK-293T C233(1.11)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C233(1.03)  LDD1511  [3]
 LDCM0275  AKOS034007705 HEK-293T C233(1.08)  LDD1514  [3]
 LDCM0379  CL11 HEK-293T C233(0.95)  LDD1583  [3]
 LDCM0390  CL12 HEK-293T C233(0.92)  LDD1594  [3]
 LDCM0409  CL21 HEK-293T C233(0.91)  LDD1613  [3]
 LDCM0411  CL23 HEK-293T C233(1.57)  LDD1615  [3]
 LDCM0412  CL24 HEK-293T C233(0.94)  LDD1616  [3]
 LDCM0422  CL33 HEK-293T C233(0.81)  LDD1626  [3]
 LDCM0424  CL35 HEK-293T C233(1.10)  LDD1628  [3]
 LDCM0425  CL36 HEK-293T C233(0.78)  LDD1629  [3]
 LDCM0435  CL45 HEK-293T C233(1.16)  LDD1639  [3]
 LDCM0437  CL47 HEK-293T C233(1.19)  LDD1641  [3]
 LDCM0438  CL48 HEK-293T C233(0.84)  LDD1642  [3]
 LDCM0448  CL57 HEK-293T C233(0.90)  LDD1651  [3]
 LDCM0450  CL59 HEK-293T C233(1.04)  LDD1653  [3]
 LDCM0452  CL60 HEK-293T C233(0.90)  LDD1655  [3]
 LDCM0461  CL69 HEK-293T C233(0.98)  LDD1664  [3]
 LDCM0464  CL71 HEK-293T C233(1.08)  LDD1667  [3]
 LDCM0465  CL72 HEK-293T C233(0.77)  LDD1668  [3]
 LDCM0475  CL81 HEK-293T C233(0.97)  LDD1678  [3]
 LDCM0477  CL83 HEK-293T C233(1.06)  LDD1680  [3]
 LDCM0478  CL84 HEK-293T C233(0.79)  LDD1681  [3]
 LDCM0484  CL9 HEK-293T C233(1.09)  LDD1687  [3]
 LDCM0488  CL93 HEK-293T C233(0.74)  LDD1691  [3]
 LDCM0490  CL95 HEK-293T C233(0.90)  LDD1693  [3]
 LDCM0491  CL96 HEK-293T C233(0.92)  LDD1694  [3]
 LDCM0022  KB02 AGS C654(1.50)  LDD2263  [1]
 LDCM0023  KB03 AGS C654(1.56)  LDD2680  [1]
 LDCM0024  KB05 NALM-6 C654(1.98)  LDD3339  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone deacetylase 1 (HDAC1) Histone deacetylase family Q13547
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402