Details of the Target

General Information of Target

Target ID LDTP10547
Target Name Sentrin-specific protease 8 (SENP8)
Gene Name SENP8
Gene ID 123228
Synonyms
DEN1; NEDP1; PRSC2; Sentrin-specific protease 8; EC 3.4.22.-; Deneddylase-1; NEDD8-specific protease 1; Protease, cysteine 2; Sentrin/SUMO-specific protease SENP8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDGSGPFSCPICLEPLREPVTLPCGHNFCLACLGALWPHRGASGAGGPGGAARCPLCQEP
FPDGLQLRKNHTLSELLQLRQGSGPGSGPGPAPALAPEPSAPSALPSVPEPSAPCAPEPW
PAGEEPVRCDACPEGAALPAALSCLSCLASFCPAHLGPHERSPALRGHRLVPPLRRLEES
LCPRHLRPLERYCRAERVCLCEACAAQEHRGHELVPLEQERALQEAEQSKVLSAVEDRMD
ELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRS
QGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFT
KSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPR
DYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRG
TYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPL
PHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSH
FAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Peptidase C48 family
Function Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
TTD ID
T13928
Uniprot ID
Q96LD8
DrugMap ID
TTMBUHI
Ensemble ID
ENST00000340912.6
HGNC ID
HGNC:22992
ChEMBL ID
CHEMBL1741207

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C64(1.08)  LDD1492  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C158
 Probe Info  		15.24  LDD1838  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C64(0.97); C163(1.32)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C163(0.94)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C64(0.99); C163(1.10)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C64(1.07)  LDD1510  [1]
 LDCM0270  AC15 HEK-293T C64(0.90); C163(1.02)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C64(0.96); C163(1.21)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C163(1.03)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C64(0.92); C163(0.98)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C163(1.00)  LDD1518  [1]
 LDCM0280  AC20 HEK-293T C64(1.02)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C163(1.06)  LDD1520  [1]
 LDCM0283  AC23 HEK-293T C64(0.96); C163(1.00)  LDD1522  [1]
 LDCM0285  AC25 HEK-293T C64(0.93); C163(1.25)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C163(0.92)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C64(0.83); C163(1.07)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C64(1.12)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C163(0.99)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C64(0.81); C163(1.08)  LDD1529  [1]
 LDCM0292  AC31 HEK-293T C64(0.95); C163(1.08)  LDD1531  [1]
 LDCM0294  AC33 HEK-293T C64(1.00); C163(1.27)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C163(1.10)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C64(0.91); C163(1.16)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C64(1.08)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C163(1.06)  LDD1537  [1]
 LDCM0300  AC39 HEK-293T C64(0.95); C163(1.02)  LDD1539  [1]
 LDCM0301  AC4 HEK-293T C64(1.30)  LDD1540  [1]
 LDCM0303  AC41 HEK-293T C64(0.96); C163(1.34)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C163(0.88)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C64(0.98); C163(0.98)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C64(1.11)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C163(0.97)  LDD1546  [1]
 LDCM0309  AC47 HEK-293T C64(1.03); C163(1.17)  LDD1548  [1]
 LDCM0311  AC49 HEK-293T C64(1.01); C163(1.28)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C163(0.96)  LDD1551  [1]
 LDCM0313  AC50 HEK-293T C163(1.03)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C64(0.95); C163(1.06)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C64(1.07)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C163(1.09)  LDD1555  [1]
 LDCM0318  AC55 HEK-293T C64(1.12); C163(1.16)  LDD1557  [1]
 LDCM0320  AC57 HEK-293T C64(0.78); C163(1.16)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C163(1.04)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C64(0.83); C163(1.00)  LDD1561  [1]
 LDCM0324  AC60 HEK-293T C64(1.11)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C163(1.09)  LDD1564  [1]
 LDCM0327  AC63 HEK-293T C64(0.96); C163(0.95)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C64(0.90); C163(0.99)  LDD1568  [1]
 LDCM0248  AKOS034007472 HEK-293T C163(1.13)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C64(0.83); C163(1.26)  LDD1570  [1]
 LDCM0367  CL1 HEK-293T C163(1.17)  LDD1571  [1]
 LDCM0370  CL101 HEK-293T C163(1.06)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C64(0.91); C163(1.21)  LDD1575  [1]
 LDCM0372  CL103 HEK-293T C64(0.85); C163(1.01)  LDD1576  [1]
 LDCM0374  CL105 HEK-293T C163(1.08)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C64(0.89); C163(1.06)  LDD1579  [1]
 LDCM0376  CL107 HEK-293T C64(0.97); C163(0.89)  LDD1580  [1]
 LDCM0378  CL109 HEK-293T C163(1.15)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C64(1.10); C163(1.15)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C64(0.97); C163(0.96)  LDD1584  [1]
 LDCM0381  CL111 HEK-293T C64(0.94); C163(0.95)  LDD1585  [1]
 LDCM0383  CL113 HEK-293T C163(0.97)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C64(0.87); C163(0.53)  LDD1588  [1]
 LDCM0385  CL115 HEK-293T C64(0.96); C163(0.93)  LDD1589  [1]
 LDCM0387  CL117 HEK-293T C163(0.97)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C64(0.95); C163(1.11)  LDD1592  [1]
 LDCM0389  CL119 HEK-293T C64(0.88); C163(0.91)  LDD1593  [1]
 LDCM0392  CL121 HEK-293T C163(1.11)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C64(1.07); C163(0.94)  LDD1597  [1]
 LDCM0394  CL123 HEK-293T C64(0.90); C163(1.05)  LDD1598  [1]
 LDCM0396  CL125 HEK-293T C163(1.09)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C64(0.87); C163(1.02)  LDD1601  [1]
 LDCM0398  CL127 HEK-293T C64(0.98); C163(0.96)  LDD1602  [1]
 LDCM0400  CL13 HEK-293T C163(1.14)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C64(0.87); C163(1.09)  LDD1605  [1]
 LDCM0402  CL15 HEK-293T C64(0.92); C163(0.83)  LDD1606  [1]
 LDCM0404  CL17 HEK-293T C64(1.07); C163(1.17)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C163(1.04)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C64(1.02); C163(1.12)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C64(0.96); C163(0.98)  LDD1611  [1]
 LDCM0408  CL20 HEK-293T C64(1.50)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C163(1.11)  LDD1613  [1]
 LDCM0411  CL23 HEK-293T C64(1.22); C163(1.05)  LDD1615  [1]
 LDCM0413  CL25 HEK-293T C163(1.00)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C64(1.01); C163(0.99)  LDD1618  [1]
 LDCM0415  CL27 HEK-293T C64(0.75); C163(0.95)  LDD1619  [1]
 LDCM0417  CL29 HEK-293T C64(0.85); C163(1.14)  LDD1621  [1]
 LDCM0418  CL3 HEK-293T C64(0.89); C163(0.94)  LDD1622  [1]
 LDCM0419  CL30 HEK-293T C163(0.88)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C64(0.94); C163(1.06)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C64(1.28)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C163(1.22)  LDD1626  [1]
 LDCM0424  CL35 HEK-293T C64(1.00); C163(1.08)  LDD1628  [1]
 LDCM0426  CL37 HEK-293T C163(0.91)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C64(0.98); C163(1.17)  LDD1632  [1]
 LDCM0431  CL41 HEK-293T C64(0.84); C163(1.32)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C163(0.98)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C64(0.99); C163(1.06)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C64(1.11)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C163(1.12)  LDD1639  [1]
 LDCM0437  CL47 HEK-293T C64(1.25); C163(1.01)  LDD1641  [1]
 LDCM0439  CL49 HEK-293T C163(0.97)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C64(0.80); C163(1.26)  LDD1644  [1]
 LDCM0441  CL50 HEK-293T C64(0.91); C163(1.06)  LDD1645  [1]
 LDCM0444  CL53 HEK-293T C64(0.99); C163(1.51)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C163(0.99)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C64(0.98); C163(1.18)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C64(1.36)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C163(1.04)  LDD1651  [1]
 LDCM0450  CL59 HEK-293T C64(1.23); C163(1.15)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C163(1.11)  LDD1654  [1]
 LDCM0453  CL61 HEK-293T C163(1.17)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C64(0.93); C163(1.03)  LDD1657  [1]
 LDCM0455  CL63 HEK-293T C64(0.85); C163(0.89)  LDD1658  [1]
 LDCM0457  CL65 HEK-293T C64(0.81); C163(1.09)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C163(0.86)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C64(0.94); C163(1.19)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C64(1.39)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C163(1.11)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C64(0.91); C163(1.08)  LDD1665  [1]
 LDCM0464  CL71 HEK-293T C64(1.15); C163(1.11)  LDD1667  [1]
 LDCM0466  CL73 HEK-293T C163(1.08)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C64(0.96); C163(1.03)  LDD1670  [1]
 LDCM0470  CL77 HEK-293T C64(0.69); C163(1.25)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C163(0.52)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C64(1.01); C163(1.08)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C64(1.48)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C64(1.17)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C163(1.05)  LDD1678  [1]
 LDCM0477  CL83 HEK-293T C64(1.14); C163(0.97)  LDD1680  [1]
 LDCM0479  CL85 HEK-293T C163(1.01)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C64(0.88); C163(1.02)  LDD1683  [1]
 LDCM0481  CL87 HEK-293T C64(0.93); C163(1.11)  LDD1684  [1]
 LDCM0483  CL89 HEK-293T C64(0.87); C163(1.15)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C163(1.13)  LDD1687  [1]
 LDCM0485  CL90 HEK-293T C163(0.95)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C64(1.14); C163(1.06)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C64(1.43)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C163(1.25)  LDD1691  [1]
 LDCM0490  CL95 HEK-293T C64(1.07); C163(1.06)  LDD1693  [1]
 LDCM0492  CL97 HEK-293T C163(1.12)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C64(1.00); C163(1.10)  LDD1696  [1]
 LDCM0494  CL99 HEK-293T C64(0.95); C163(0.93)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C64(0.99); C163(1.09)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C64(0.86); C163(0.96)  LDD1671  [1]
 LDCM0427  Fragment51 HEK-293T C64(0.82); C163(0.94)  LDD1631  [1]
 LDCM0022  KB02 HEK-293T C64(1.08)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C64(1.03)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C64(0.96)  LDD1502  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Beta-chimaerin (CHN2) . P52757

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
St074946 Small molecular drug D05TNT

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587