Details of the Target

General Information of Target

Target ID LDTP10257
Target Name Myotubularin-related protein 8 (MTMR8)
Gene Name MTMR8
Gene ID 55613
Synonyms
Myotubularin-related protein 8; Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; EC 3.1.3.95; Phosphatidylinositol-3-phosphate phosphatase; EC 3.1.3.64
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFVARSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWDKSESGDWHCTASWKTHSGSV
WRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVK
FAPKHMGLMLATCSADGIVRIYEAPDVMNLSQWSLQHEISCKLSCSCISWNPSSSRAHSP
MIAVGSDDSSPNAMAKVQIFEYNENTRKYAKAETLMTVTDPVHDIAFAPNLGRSFHILAI
ATKDVRIFTLKPVRKELTSSGGPTKFEIHIVAQFDNHNSQVWRVSWNITGTVLASSGDDG
CVRLWKANYMDNWKCTGILKGNGSPVNGSSQQGTSNPSLGSTIPSLQNSLNGSSAGRKHS
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily
Subcellular location
Nucleus envelope
Function
Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. In complex with MTMR9, negatively regulates autophagy.
Uniprot ID
Q96EF0
Ensemble ID
ENST00000374852.4
HGNC ID
HGNC:16825

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL78 Deletion: p.G689_T695del .
G361 Substitution: p.N669_L670delinsKM .
HCC1937 SNV: p.E454K .
HCT15 SNV: p.W496L .
HEC1B SNV: p.D340G .
HT SNV: p.D681G .
HT115 SNV: p.A675S .
MCC13 SNV: p.R541C .
MDAMB453 SNV: p.F322L .
MDST8 SNV: p.L388I .
MEWO SNV: p.N259I .
NCIH716 Substitution: p.N669_L670delinsKM .
NCIH82 SNV: p.G555V .
RH41 SNV: p.W407C .
TE8 Deletion: p.C216VfsTer5 .
TOV21G SNV: p.S458Y .
U2OS SNV: p.S179R; p.D219N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C216(1.01)  LDD1512  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C216(1.01)  LDD1512  [1]
 LDCM0270  AC15 HEK-293T C216(0.85)  LDD1513  [1]
 LDCM0282  AC22 HEK-293T C216(0.98)  LDD1521  [1]
 LDCM0283  AC23 HEK-293T C216(1.10)  LDD1522  [1]
 LDCM0291  AC30 HEK-293T C216(1.01)  LDD1530  [1]
 LDCM0292  AC31 HEK-293T C216(0.99)  LDD1531  [1]
 LDCM0299  AC38 HEK-293T C216(1.08)  LDD1538  [1]
 LDCM0300  AC39 HEK-293T C216(1.26)  LDD1539  [1]
 LDCM0308  AC46 HEK-293T C216(1.16)  LDD1547  [1]
 LDCM0309  AC47 HEK-293T C216(0.93)  LDD1548  [1]
 LDCM0317  AC54 HEK-293T C216(0.95)  LDD1556  [1]
 LDCM0318  AC55 HEK-293T C216(1.10)  LDD1557  [1]
 LDCM0323  AC6 HEK-293T C216(1.10)  LDD1562  [1]
 LDCM0326  AC62 HEK-293T C216(1.10)  LDD1565  [1]
 LDCM0327  AC63 HEK-293T C216(1.28)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C216(1.00)  LDD1568  [1]
 LDCM0367  CL1 HEK-293T C216(1.03)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C216(1.12)  LDD1572  [1]
 LDCM0369  CL100 HEK-293T C216(0.94)  LDD1573  [1]
 LDCM0370  CL101 HEK-293T C216(1.07)  LDD1574  [1]
 LDCM0372  CL103 HEK-293T C216(1.09)  LDD1576  [1]
 LDCM0373  CL104 HEK-293T C216(1.19)  LDD1577  [1]
 LDCM0374  CL105 HEK-293T C216(1.14)  LDD1578  [1]
 LDCM0376  CL107 HEK-293T C216(0.85)  LDD1580  [1]
 LDCM0377  CL108 HEK-293T C216(1.20)  LDD1581  [1]
 LDCM0378  CL109 HEK-293T C216(0.82)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C216(0.94)  LDD1583  [1]
 LDCM0381  CL111 HEK-293T C216(1.26)  LDD1585  [1]
 LDCM0382  CL112 HEK-293T C216(1.33)  LDD1586  [1]
 LDCM0383  CL113 HEK-293T C216(1.09)  LDD1587  [1]
 LDCM0385  CL115 HEK-293T C216(1.03)  LDD1589  [1]
 LDCM0386  CL116 HEK-293T C216(1.14)  LDD1590  [1]
 LDCM0387  CL117 HEK-293T C216(1.05)  LDD1591  [1]
 LDCM0389  CL119 HEK-293T C216(1.15)  LDD1593  [1]
 LDCM0391  CL120 HEK-293T C216(1.30)  LDD1595  [1]
 LDCM0392  CL121 HEK-293T C216(0.98)  LDD1596  [1]
 LDCM0394  CL123 HEK-293T C216(1.02)  LDD1598  [1]
 LDCM0395  CL124 HEK-293T C216(1.28)  LDD1599  [1]
 LDCM0396  CL125 HEK-293T C216(0.77)  LDD1600  [1]
 LDCM0398  CL127 HEK-293T C216(1.08)  LDD1602  [1]
 LDCM0399  CL128 HEK-293T C216(1.18)  LDD1603  [1]
 LDCM0400  CL13 HEK-293T C216(0.75)  LDD1604  [1]
 LDCM0402  CL15 HEK-293T C216(0.83)  LDD1606  [1]
 LDCM0403  CL16 HEK-293T C216(1.07)  LDD1607  [1]
 LDCM0410  CL22 HEK-293T C216(0.95)  LDD1614  [1]
 LDCM0411  CL23 HEK-293T C216(0.93)  LDD1615  [1]
 LDCM0413  CL25 HEK-293T C216(1.04)  LDD1617  [1]
 LDCM0415  CL27 HEK-293T C216(1.29)  LDD1619  [1]
 LDCM0416  CL28 HEK-293T C216(1.13)  LDD1620  [1]
 LDCM0418  CL3 HEK-293T C216(1.10)  LDD1622  [1]
 LDCM0423  CL34 HEK-293T C216(0.82)  LDD1627  [1]
 LDCM0424  CL35 HEK-293T C216(0.87)  LDD1628  [1]
 LDCM0426  CL37 HEK-293T C216(1.11)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C216(0.94)  LDD1632  [1]
 LDCM0429  CL4 HEK-293T C216(1.03)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C216(1.10)  LDD1634  [1]
 LDCM0436  CL46 HEK-293T C216(1.06)  LDD1640  [1]
 LDCM0437  CL47 HEK-293T C216(1.10)  LDD1641  [1]
 LDCM0439  CL49 HEK-293T C216(0.94)  LDD1643  [1]
 LDCM0443  CL52 HEK-293T C216(1.17)  LDD1646  [1]
 LDCM0449  CL58 HEK-293T C216(0.86)  LDD1652  [1]
 LDCM0450  CL59 HEK-293T C216(1.00)  LDD1653  [1]
 LDCM0453  CL61 HEK-293T C216(0.89)  LDD1656  [1]
 LDCM0455  CL63 HEK-293T C216(1.09)  LDD1658  [1]
 LDCM0456  CL64 HEK-293T C216(1.01)  LDD1659  [1]
 LDCM0463  CL70 HEK-293T C216(1.01)  LDD1666  [1]
 LDCM0464  CL71 HEK-293T C216(1.07)  LDD1667  [1]
 LDCM0466  CL73 HEK-293T C216(0.85)  LDD1669  [1]
 LDCM0469  CL76 HEK-293T C216(1.21)  LDD1672  [1]
 LDCM0476  CL82 HEK-293T C216(1.11)  LDD1679  [1]
 LDCM0477  CL83 HEK-293T C216(1.01)  LDD1680  [1]
 LDCM0479  CL85 HEK-293T C216(1.29)  LDD1682  [1]
 LDCM0481  CL87 HEK-293T C216(1.12)  LDD1684  [1]
 LDCM0482  CL88 HEK-293T C216(1.29)  LDD1685  [1]
 LDCM0489  CL94 HEK-293T C216(0.91)  LDD1692  [1]
 LDCM0490  CL95 HEK-293T C216(0.82)  LDD1693  [1]
 LDCM0492  CL97 HEK-293T C216(0.95)  LDD1695  [1]
 LDCM0494  CL99 HEK-293T C216(1.16)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C216(0.86)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C216(1.07)  LDD1671  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myotubularin-related protein 9 (MTMR9) Protein-tyrosine phosphatase family Q96QG7

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019