Details of the Target

General Information of Target

Target ID LDTP10142
Target Name Alkylated DNA repair protein alkB homolog 8 (ALKBH8)
Gene Name ALKBH8
Gene ID 91801
Synonyms
ABH8; Alkylated DNA repair protein alkB homolog 8; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA; carboxymethyluridine(34)-5-O)-methyltransferase ABH8; EC 2.1.1.229
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADHNPDSDSTPRTLLRRVLDTADPRTPRRPRSARAGARRALLETASPRKLSGQTRTIAR
GRSHGARSVGRSAHIQASGHLEEQTPRTLLKNILLTAPESSILMPESVVKPVPAPQAVQP
SRQESSCGSLELQLPELEPPTTLAPGLLAPGRRKQRLRLSVFQQGVDQGLSLSQEPQGNA
DASSLTRSLNLTFATPLQPQSVQRPGLARRPPARRAVDVGAFLRDLRDTSLAPPNIVLED
TQPFSQPMVGSPNVYHSLPCTPHTGAEDAEQAAGRKTQSSGPGLQKNSPGKPAQFLAGEA
EEVNAFALGFLSTSSGVSGEDEVEPLHDGVEEAEKKMEEEGVSVSEMEATGAQGPSRVEE
AEGHTEVTEAEGSQGTAEADGPGASSGDEDASGRAASPESASSTPESLQARRHHQFLEPA
PAPGAAVLSSEPAEPLLVRHPPRPRTTGPRPRQDPHKAGLSHYVKLFSFYAKMPMERKAL
EMVEKCLDKYFQHLCDDLEVFAAHAGRKTVKPEDLELLMRRQGLVTDQVSLHVLVERHLP
LEYRQLLIPCAYSGNSVFPAQ
Target Bioclass
Enzyme
Family
AlkB family
Subcellular location
Cytoplasm
Function
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Uniprot ID
Q96BT7
Ensemble ID
ENST00000260318.6
HGNC ID
HGNC:25189

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.Y588H .
HCT116 SNV: p.I660V .
HT29 SNV: p.T37K .
KYSE150 SNV: p.P558R .
MFE319 SNV: p.V582I .
NCIH1703 SNV: p.D316E .
REH SNV: p.S251Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C299(3.45)  LDD3407  [1]
IPM
 Probe Info  		C296(0.63)  LDD1701  [2]
IA-alkyne
 Probe Info  		C442(0.00); C68(0.00)  LDD0165  [3]
HHS-465
 Probe Info  		N.A.  LDD2240  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C296(1.01)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C442(1.02); C296(1.05)  LDD1509  [5]
 LDCM0277  AC18 HEK-293T C296(1.07)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C442(0.91); C296(0.84)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C296(1.02)  LDD1518  [5]
 LDCM0284  AC24 HEK-293T C296(0.97)  LDD1523  [5]
 LDCM0286  AC26 HEK-293T C296(0.99)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C442(1.02); C296(1.08)  LDD1526  [5]
 LDCM0290  AC3 HEK-293T C442(0.96); C296(1.04)  LDD1529  [5]
 LDCM0293  AC32 HEK-293T C296(0.92)  LDD1532  [5]
 LDCM0295  AC34 HEK-293T C296(1.07)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C442(1.08); C296(1.15)  LDD1535  [5]
 LDCM0302  AC40 HEK-293T C296(1.02)  LDD1541  [5]
 LDCM0304  AC42 HEK-293T C296(1.07)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C442(1.04); C296(1.10)  LDD1544  [5]
 LDCM0310  AC48 HEK-293T C296(0.98)  LDD1549  [5]
 LDCM0313  AC50 HEK-293T C296(1.11)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C442(1.04); C296(1.14)  LDD1553  [5]
 LDCM0319  AC56 HEK-293T C296(0.95)  LDD1558  [5]
 LDCM0321  AC58 HEK-293T C296(1.16)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C442(0.93); C296(1.10)  LDD1561  [5]
 LDCM0328  AC64 HEK-293T C296(1.04)  LDD1567  [5]
 LDCM0345  AC8 HEK-293T C296(1.07)  LDD1569  [5]
 LDCM0275  AKOS034007705 HEK-293T C296(0.96)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C639(1.04)  LDD1571  [5]
 LDCM0370  CL101 HEK-293T C639(1.02)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C442(0.95)  LDD1575  [5]
 LDCM0374  CL105 HEK-293T C639(1.06)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C442(1.07)  LDD1579  [5]
 LDCM0378  CL109 HEK-293T C639(0.97)  LDD1582  [5]
 LDCM0380  CL110 HEK-293T C442(1.05)  LDD1584  [5]
 LDCM0383  CL113 HEK-293T C639(1.07)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C442(0.94)  LDD1588  [5]
 LDCM0387  CL117 HEK-293T C639(1.03)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C442(1.15)  LDD1592  [5]
 LDCM0390  CL12 HEK-293T C296(1.16)  LDD1594  [5]
 LDCM0392  CL121 HEK-293T C639(0.99)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C442(0.95)  LDD1597  [5]
 LDCM0396  CL125 HEK-293T C639(0.96)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C442(1.04)  LDD1601  [5]
 LDCM0400  CL13 HEK-293T C639(1.04)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C442(1.30)  LDD1605  [5]
 LDCM0405  CL18 HEK-293T C296(1.21)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C442(1.10); C296(0.92)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C442(1.09)  LDD1611  [5]
 LDCM0412  CL24 HEK-293T C296(1.11)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C639(0.88)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C442(1.20)  LDD1618  [5]
 LDCM0419  CL30 HEK-293T C296(1.01)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C442(1.12); C296(0.77)  LDD1624  [5]
 LDCM0425  CL36 HEK-293T C296(1.08)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C639(0.90)  LDD1630  [5]
 LDCM0432  CL42 HEK-293T C296(0.94)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C442(1.05); C296(0.84)  LDD1637  [5]
 LDCM0438  CL48 HEK-293T C296(1.20)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C639(0.82)  LDD1643  [5]
 LDCM0441  CL50 HEK-293T C442(1.17)  LDD1645  [5]
 LDCM0445  CL54 HEK-293T C296(1.25)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C442(1.09); C296(0.82)  LDD1649  [5]
 LDCM0451  CL6 HEK-293T C296(1.45)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C296(1.10)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C639(0.85)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C442(1.16)  LDD1657  [5]
 LDCM0458  CL66 HEK-293T C296(1.09)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C442(1.02); C296(0.91)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C442(0.92); C296(0.89)  LDD1665  [5]
 LDCM0465  CL72 HEK-293T C296(0.97)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C639(1.01)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C442(1.00)  LDD1670  [5]
 LDCM0471  CL78 HEK-293T C296(0.96)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C442(1.04); C296(0.88)  LDD1675  [5]
 LDCM0478  CL84 HEK-293T C296(1.25)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C639(0.95)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C442(1.13)  LDD1683  [5]
 LDCM0485  CL90 HEK-293T C296(1.27)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C442(1.03); C296(0.97)  LDD1689  [5]
 LDCM0491  CL96 HEK-293T C296(1.32)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C639(1.04)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C442(0.92)  LDD1696  [5]
 LDCM0574  Fragment12 Ramos C568(0.38)  LDD2191  [6]
 LDCM0575  Fragment13 Ramos C568(0.44)  LDD2192  [6]
 LDCM0576  Fragment14 Ramos C568(0.67)  LDD2193  [6]
 LDCM0579  Fragment20 Ramos C568(0.35)  LDD2194  [6]
 LDCM0580  Fragment21 Ramos C568(0.60)  LDD2195  [6]
 LDCM0582  Fragment23 Ramos C568(0.57)  LDD2196  [6]
 LDCM0578  Fragment27 Ramos C568(0.46)  LDD2197  [6]
 LDCM0586  Fragment28 Ramos C568(0.77)  LDD2198  [6]
 LDCM0588  Fragment30 Ramos C568(0.64)  LDD2199  [6]
 LDCM0589  Fragment31 Ramos C568(0.45)  LDD2200  [6]
 LDCM0590  Fragment32 Ramos C568(0.79)  LDD2201  [6]
 LDCM0468  Fragment33 Ramos C568(0.56)  LDD2202  [6]
 LDCM0596  Fragment38 Ramos C568(0.25)  LDD2203  [6]
 LDCM0566  Fragment4 Ramos C568(0.70)  LDD2184  [6]
 LDCM0427  Fragment51 HEK-293T C442(1.12)  LDD1631  [5]
 LDCM0610  Fragment52 Ramos C568(0.69)  LDD2204  [6]
 LDCM0614  Fragment56 Ramos C568(0.60)  LDD2205  [6]
 LDCM0569  Fragment7 Ramos C568(0.89)  LDD2186  [6]
 LDCM0571  Fragment9 Ramos C568(0.46)  LDD2188  [6]
 LDCM0022  KB02 HEK-293T C442(0.92)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C442(1.10)  LDD1497  [5]
 LDCM0024  KB05 RKO C299(3.45)  LDD3407  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Microfibrillar-associated protein 1 (MFAP1) MFAP1 family P55081
Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) TRM112 family Q9UI30
PRKCA-binding protein (PICK1) . Q9NRD5

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578