Details of the Target

General Information of Target

Target ID LDTP09653
Target Name Protein spire homolog 2 (SPIRE2)
Gene Name SPIRE2
Gene ID 84501
Synonyms
KIAA1832; SPIR2; Protein spire homolog 2; Spir-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARAGSCGGAAAGAGRPEPWELSLEEVLKAYEQPLNEEQAWAVCFQGCRGLRGSPGRRLR
DTGDLLLRGDGSVGAREPEAAEPATMVVPLASSEAQTVQSLGFAIYRALDWGLDESEERE
LSPQLERLIDLMANNDSEDSGCGAADEGYGGPEEEEEAEGVPRSVRTFAQAMRLCAARLT
DPRGAQAHYQAVCRALFVETLELRAFLARVREAKEMLQKLREDEPHLETPRAELDSLGHT
DWARLWVQLMRELRRGVKLKKVQEQEFNPLPTEFQLTPFEMLMQDIRARNYKLRKVMVDG
DIPPRVKKDAHELILDFIRSRPPLKQVSERRLRPLPPKQRSLHEKILEEIKQERRLRPVR
GEGWAARGFGSLPCILNACSGDAKSTSCINLSVTDAGGSAQRPRPRVLLKAPTLAEMEEM
NTSEEEESPCGEVTLKRDRSFSEHDLAQLRSEVASGLQSATHPPGGTEPPRPRAGSAHVW
RPGSRDQGTCPASVSDPSHPLLSNRGSSGDRPEASMTPDAKHLWLEFSHPVESLALTVEE
VMDVRRVLVKAEMEKFLQNKELFSSLKKGKICCCCRAKFPLFSWPPSCLFCKRAVCTSCS
IKMKMPSKKFGHIPVYTLGFESPQRVSAAKTAPIQRRDIFQSLQGPQWQSVEEAFPHIYS
HGCVLKDVCSECTSFVADVVRSSRKSVDVLNTTPRRSRQTQSLYIPNTRTLDFK
Target Bioclass
Other
Family
Spire family
Subcellular location
Cytoplasm, cytoskeleton
Function
Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage.
Uniprot ID
Q8WWL2
Ensemble ID
ENST00000378247.8
HGNC ID
HGNC:30623

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DOTC24510 SNV: p.D61N .
HEC1 SNV: p.G662D .
HS578T SNV: p.G160A DBIA    Probe Info 
L428 SNV: p.D309N .
LS513 SNV: p.R505W .
MOLT4 SNV: p.M283I; p.E552K .
NCIH1155 SNV: p.A95V DBIA    Probe Info 
P31FUJ SNV: p.Q186Ter .
RKO SNV: p.G256A DBIA    Probe Info 
RL952 SNV: p.E233Ter .
SNU5 SNV: p.C44S .
WM793 SNV: p.R289G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C62(2.67)  LDD3332  [1]
TFBX
 Probe Info  		N.A.  LDD0027  [2]
IPM
 Probe Info  		N.A.  LDD0147  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C374(1.10)  LDD1507  [3]
 LDCM0259  AC14 HEK-293T C374(1.20)  LDD1512  [3]
 LDCM0276  AC17 HEK-293T C374(1.24)  LDD1515  [3]
 LDCM0281  AC21 HEK-293T C374(1.03)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C374(0.85)  LDD1521  [3]
 LDCM0284  AC24 HEK-293T C663(1.07)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C374(1.31)  LDD1524  [3]
 LDCM0289  AC29 HEK-293T C374(1.15)  LDD1528  [3]
 LDCM0291  AC30 HEK-293T C374(1.30)  LDD1530  [3]
 LDCM0293  AC32 HEK-293T C663(1.11)  LDD1532  [3]
 LDCM0294  AC33 HEK-293T C374(1.13)  LDD1533  [3]
 LDCM0298  AC37 HEK-293T C374(1.04)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C374(1.05)  LDD1538  [3]
 LDCM0302  AC40 HEK-293T C663(0.99)  LDD1541  [3]
 LDCM0303  AC41 HEK-293T C374(1.27)  LDD1542  [3]
 LDCM0307  AC45 HEK-293T C374(1.29)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C374(1.07)  LDD1547  [3]
 LDCM0310  AC48 HEK-293T C663(1.00)  LDD1549  [3]
 LDCM0311  AC49 HEK-293T C374(1.14)  LDD1550  [3]
 LDCM0312  AC5 HEK-293T C374(0.89)  LDD1551  [3]
 LDCM0316  AC53 HEK-293T C374(1.03)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C374(0.84)  LDD1556  [3]
 LDCM0319  AC56 HEK-293T C663(1.00)  LDD1558  [3]
 LDCM0320  AC57 HEK-293T C374(1.12)  LDD1559  [3]
 LDCM0323  AC6 HEK-293T C374(1.13)  LDD1562  [3]
 LDCM0325  AC61 HEK-293T C374(1.20)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C374(1.09)  LDD1565  [3]
 LDCM0328  AC64 HEK-293T C663(1.03)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C663(1.05)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C374(1.37)  LDD1511  [3]
 LDCM0356  AKOS034007680 HEK-293T C374(1.14)  LDD1570  [3]
 LDCM0275  AKOS034007705 HEK-293T C663(1.07)  LDD1514  [3]
 LDCM0368  CL10 HEK-293T C374(1.69)  LDD1572  [3]
 LDCM0390  CL12 HEK-293T C663(0.87)  LDD1594  [3]
 LDCM0404  CL17 HEK-293T C374(1.62)  LDD1608  [3]
 LDCM0409  CL21 HEK-293T C374(1.29)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C374(0.91)  LDD1614  [3]
 LDCM0412  CL24 HEK-293T C663(0.94)  LDD1616  [3]
 LDCM0417  CL29 HEK-293T C374(1.15)  LDD1621  [3]
 LDCM0422  CL33 HEK-293T C374(1.18)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C374(1.06)  LDD1627  [3]
 LDCM0425  CL36 HEK-293T C663(1.03)  LDD1629  [3]
 LDCM0431  CL41 HEK-293T C374(1.28)  LDD1635  [3]
 LDCM0435  CL45 HEK-293T C374(1.27)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C374(1.04)  LDD1640  [3]
 LDCM0438  CL48 HEK-293T C663(1.00)  LDD1642  [3]
 LDCM0440  CL5 HEK-293T C374(0.99)  LDD1644  [3]
 LDCM0444  CL53 HEK-293T C374(1.31)  LDD1647  [3]
 LDCM0448  CL57 HEK-293T C374(1.03)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C374(1.20)  LDD1652  [3]
 LDCM0452  CL60 HEK-293T C663(0.82)  LDD1655  [3]
 LDCM0457  CL65 HEK-293T C374(1.49)  LDD1660  [3]
 LDCM0461  CL69 HEK-293T C374(1.02)  LDD1664  [3]
 LDCM0463  CL70 HEK-293T C374(1.04)  LDD1666  [3]
 LDCM0465  CL72 HEK-293T C663(0.88)  LDD1668  [3]
 LDCM0470  CL77 HEK-293T C374(1.66)  LDD1673  [3]
 LDCM0475  CL81 HEK-293T C374(1.09)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C374(1.11)  LDD1679  [3]
 LDCM0478  CL84 HEK-293T C663(1.01)  LDD1681  [3]
 LDCM0483  CL89 HEK-293T C374(1.17)  LDD1686  [3]
 LDCM0484  CL9 HEK-293T C374(1.13)  LDD1687  [3]
 LDCM0488  CL93 HEK-293T C374(1.05)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C374(0.93)  LDD1692  [3]
 LDCM0491  CL96 HEK-293T C663(0.94)  LDD1694  [3]
 LDCM0022  KB02 22RV1 C62(1.76)  LDD2243  [1]
 LDCM0023  KB03 A101D C62(2.45)  LDD2667  [1]
 LDCM0024  KB05 MKN45 C62(2.67)  LDD3332  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Latrunculin B . DB08080

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402