Details of the Target

General Information of Target

Target ID LDTP09534
Target Name WAS/WASL-interacting protein family member 2 (WIPF2)
Gene Name WIPF2
Gene ID 147179
Synonyms
WICH; WIRE; WAS/WASL-interacting protein family member 2; WASP-interacting protein-related protein; WIP- and CR16-homologous protein; WIP-related protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPIPPPPPPPPGPPPPPTFHQANTEQPKLSRDEQRGRGALLQDICKGTKLKKVTNINDRS
APILEKPKGSSGGYGSGGAALQPKGGLFQGGVLKLRPVGAKDGSENLAGKPALQIPSSRA
AAPRPPVSAASGRPQDDTDSSRASLPELPRMQRPSLPDLSRPNTTSSTGMKHSSSAPPPP
PPGRRANAPPTPLPMHSSKAPAYNREKPLPPTPGQRLHPGREGPPAPPPVKPPPSPVNIR
TGPSGQSLAPPPPPYRQPPGVPNGPSSPTNESAPELPQRHNSLHRKTPGPVRGLAPPPPT
SASPSLLSNRPPPPARDPPSRGAAPPPPPPVIRNGARDAPPPPPPYRMHGSEPPSRGKPP
PPPSRTPAGPPPPPPPPLRNGHRDSITTVRSFLDDFESKYSFHPVEDFPAPEEYKHFQRI
YPSKTNRAARGAPPLPPILR
Target Bioclass
Other
Family
Verprolin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system.
Uniprot ID
Q8TF74
Ensemble ID
ENST00000323571.9
HGNC ID
HGNC:30923

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A172 SNV: p.K101E .
CCK81 SNV: p.P434H .
HCC15 SNV: p.P288L .
HEC1 SNV: p.R427Ter .
KU1919 SNV: p.K286N .
MOLT4 SNV: p.S160F .
SKMEL5 SNV: p.H20Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		8.62  LDD0066  [1]
STPyne
 Probe Info  		K286(9.82)  LDD0277  [2]
DBIA
 Probe Info  		C45(2.10)  LDD3358  [3]
HHS-475
 Probe Info  		Y74(1.01)  LDD0264  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C45(1.08)  LDD1513  [6]
 LDCM0283  AC23 HEK-293T C45(1.09)  LDD1522  [6]
 LDCM0292  AC31 HEK-293T C45(1.03)  LDD1531  [6]
 LDCM0300  AC39 HEK-293T C45(1.17)  LDD1539  [6]
 LDCM0309  AC47 HEK-293T C45(1.05)  LDD1548  [6]
 LDCM0318  AC55 HEK-293T C45(1.09)  LDD1557  [6]
 LDCM0327  AC63 HEK-293T C45(1.11)  LDD1566  [6]
 LDCM0334  AC7 HEK-293T C45(1.01)  LDD1568  [6]
 LDCM0369  CL100 HEK-293T C45(0.97)  LDD1573  [6]
 LDCM0373  CL104 HEK-293T C45(0.96)  LDD1577  [6]
 LDCM0377  CL108 HEK-293T C45(0.96)  LDD1581  [6]
 LDCM0379  CL11 HEK-293T C45(1.11)  LDD1583  [6]
 LDCM0382  CL112 HEK-293T C45(0.93)  LDD1586  [6]
 LDCM0386  CL116 HEK-293T C45(1.04)  LDD1590  [6]
 LDCM0391  CL120 HEK-293T C45(0.98)  LDD1595  [6]
 LDCM0395  CL124 HEK-293T C45(1.04)  LDD1599  [6]
 LDCM0399  CL128 HEK-293T C45(1.06)  LDD1603  [6]
 LDCM0403  CL16 HEK-293T C45(1.05)  LDD1607  [6]
 LDCM0411  CL23 HEK-293T C45(1.18)  LDD1615  [6]
 LDCM0416  CL28 HEK-293T C45(1.03)  LDD1620  [6]
 LDCM0424  CL35 HEK-293T C45(1.06)  LDD1628  [6]
 LDCM0429  CL4 HEK-293T C45(1.02)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C45(1.02)  LDD1634  [6]
 LDCM0437  CL47 HEK-293T C45(1.02)  LDD1641  [6]
 LDCM0443  CL52 HEK-293T C45(1.04)  LDD1646  [6]
 LDCM0450  CL59 HEK-293T C45(1.05)  LDD1653  [6]
 LDCM0456  CL64 HEK-293T C45(1.00)  LDD1659  [6]
 LDCM0464  CL71 HEK-293T C45(1.12)  LDD1667  [6]
 LDCM0469  CL76 HEK-293T C45(1.00)  LDD1672  [6]
 LDCM0477  CL83 HEK-293T C45(0.97)  LDD1680  [6]
 LDCM0482  CL88 HEK-293T C45(1.06)  LDD1685  [6]
 LDCM0490  CL95 HEK-293T C45(0.87)  LDD1693  [6]
 LDCM0116  HHS-0101 DM93 Y74(1.01)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y74(3.76)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y74(1.24)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y74(3.53)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y74(2.33)  LDD0268  [4]
 LDCM0022  KB02 42-MG-BA C45(1.90)  LDD2244  [3]
 LDCM0023  KB03 AGS C45(1.66)  LDD2680  [3]
 LDCM0024  KB05 NCI-H2291 C45(2.10)  LDD3358  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Golgi apparatus membrane protein TVP23 homolog A (TVP23A) TVP23 family A6NH52
Actin nucleation-promoting factor WASL (WASL) . O00401
Vinexin (SORBS3) . O60504

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402