Details of the Target

General Information of Target

Target ID LDTP09240
Target Name Tetraspanin-14 (TSPAN14)
Gene Name TSPAN14
Gene ID 81619
Synonyms
TM4SF14; Tetraspanin-14; Tspan-14; DC-TM4F2; Transmembrane 4 superfamily member 14
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MHYYRYSNAKVSCWYKYLLFSYNIIFWLAGVVFLGVGLWAWSEKGVLSDLTKVTRMHGID
PVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLIFFLELAVAVLAFLFQDWVR
DRFREFFESNIKSYRDDIDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKC
GVPFSCCVPDPAQKVVNTQCGYDVRIQLKSKWDESIFTKGCIQALESWLPRNIYIVAGVF
IAISLLQIFGIFLARTLISDIEAVKAGHHF
Target Bioclass
Transporter and channel
Family
Tetraspanin (TM4SF) family
Subcellular location
Cell membrane
Function
Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates. Negatively regulates ADAM10-mediated cleavage of GP6. Promotes ADAM10-mediated cleavage of CDH5.
Uniprot ID
Q8NG11
Ensemble ID
ENST00000372156.5
HGNC ID
HGNC:23303

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.W228Ter .
KARPAS422 SNV: p.T256P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K132(0.60)  LDD3494  [1]
BTD
 Probe Info  		C200(0.49)  LDD2099  [2]
DBIA
 Probe Info  		C200(1.51)  LDD0547  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
AOyne
 Probe Info  		11.50  LDD0443  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C200(0.41)  LDD2117  [2]
 LDCM0214  AC1 PaTu 8988t C200(0.31)  LDD1093  [3]
 LDCM0215  AC10 PaTu 8988t C200(1.03)  LDD1094  [3]
 LDCM0226  AC11 PaTu 8988t C200(1.10)  LDD1105  [3]
 LDCM0230  AC113 HCT 116 C200(1.51)  LDD0547  [3]
 LDCM0231  AC114 HCT 116 C200(1.57)  LDD0548  [3]
 LDCM0232  AC115 HCT 116 C200(1.77)  LDD0549  [3]
 LDCM0233  AC116 HCT 116 C200(1.43)  LDD0550  [3]
 LDCM0234  AC117 HCT 116 C200(1.22)  LDD0551  [3]
 LDCM0235  AC118 HCT 116 C200(1.34)  LDD0552  [3]
 LDCM0236  AC119 HCT 116 C200(1.29)  LDD0553  [3]
 LDCM0237  AC12 PaTu 8988t C200(1.60)  LDD1116  [3]
 LDCM0238  AC120 HCT 116 C200(1.48)  LDD0555  [3]
 LDCM0239  AC121 HCT 116 C200(1.42)  LDD0556  [3]
 LDCM0240  AC122 HCT 116 C200(1.35)  LDD0557  [3]
 LDCM0241  AC123 HCT 116 C200(1.40)  LDD0558  [3]
 LDCM0242  AC124 HCT 116 C200(1.21)  LDD0559  [3]
 LDCM0243  AC125 HCT 116 C200(1.44)  LDD0560  [3]
 LDCM0244  AC126 HCT 116 C200(1.21)  LDD0561  [3]
 LDCM0245  AC127 HCT 116 C200(1.20)  LDD0562  [3]
 LDCM0259  AC14 PaTu 8988t C200(1.28)  LDD1138  [3]
 LDCM0270  AC15 PaTu 8988t C200(1.33)  LDD1149  [3]
 LDCM0279  AC2 PaTu 8988t C200(0.27)  LDD1158  [3]
 LDCM0290  AC3 PaTu 8988t C200(0.33)  LDD1169  [3]
 LDCM0301  AC4 PaTu 8988t C200(0.21)  LDD1180  [3]
 LDCM0308  AC46 HCT 116 C200(0.84)  LDD0625  [3]
 LDCM0309  AC47 HCT 116 C200(0.89)  LDD0626  [3]
 LDCM0310  AC48 HCT 116 C200(0.67)  LDD0627  [3]
 LDCM0311  AC49 HCT 116 C200(0.87)  LDD0628  [3]
 LDCM0312  AC5 PaTu 8988t C200(0.24)  LDD1191  [3]
 LDCM0313  AC50 HCT 116 C200(0.92)  LDD0630  [3]
 LDCM0314  AC51 HCT 116 C200(0.82)  LDD0631  [3]
 LDCM0315  AC52 HCT 116 C200(0.79)  LDD0632  [3]
 LDCM0316  AC53 HCT 116 C200(1.01)  LDD0633  [3]
 LDCM0317  AC54 HCT 116 C200(0.68)  LDD0634  [3]
 LDCM0318  AC55 HCT 116 C200(0.87)  LDD0635  [3]
 LDCM0319  AC56 HCT 116 C200(0.83)  LDD0636  [3]
 LDCM0320  AC57 HCT 116 C200(1.21)  LDD0637  [3]
 LDCM0321  AC58 HCT 116 C200(0.93)  LDD0638  [3]
 LDCM0322  AC59 HCT 116 C200(1.38)  LDD0639  [3]
 LDCM0323  AC6 PaTu 8988t C200(1.85)  LDD1202  [3]
 LDCM0324  AC60 HCT 116 C200(1.32)  LDD0641  [3]
 LDCM0325  AC61 HCT 116 C200(1.08)  LDD0642  [3]
 LDCM0326  AC62 HCT 116 C200(0.98)  LDD0643  [3]
 LDCM0327  AC63 HCT 116 C200(1.07)  LDD0644  [3]
 LDCM0328  AC64 HCT 116 C200(1.12)  LDD0645  [3]
 LDCM0329  AC65 HCT 116 C200(1.14)  LDD0646  [3]
 LDCM0330  AC66 HCT 116 C200(1.26)  LDD0647  [3]
 LDCM0331  AC67 HCT 116 C200(0.71)  LDD0648  [3]
 LDCM0332  AC68 HCT 116 C200(1.80)  LDD0649  [3]
 LDCM0333  AC69 HCT 116 C200(1.00)  LDD0650  [3]
 LDCM0334  AC7 PaTu 8988t C200(1.30)  LDD1213  [3]
 LDCM0335  AC70 HCT 116 C200(1.19)  LDD0652  [3]
 LDCM0336  AC71 HCT 116 C200(1.25)  LDD0653  [3]
 LDCM0337  AC72 HCT 116 C200(1.45)  LDD0654  [3]
 LDCM0338  AC73 HCT 116 C200(0.76)  LDD0655  [3]
 LDCM0339  AC74 HCT 116 C200(1.16)  LDD0656  [3]
 LDCM0340  AC75 HCT 116 C200(0.73)  LDD0657  [3]
 LDCM0341  AC76 HCT 116 C200(1.50)  LDD0658  [3]
 LDCM0342  AC77 HCT 116 C200(0.95)  LDD0659  [3]
 LDCM0343  AC78 HCT 116 C200(1.27)  LDD0660  [3]
 LDCM0344  AC79 HCT 116 C200(1.31)  LDD0661  [3]
 LDCM0345  AC8 PaTu 8988t C200(1.28)  LDD1224  [3]
 LDCM0346  AC80 HCT 116 C200(1.22)  LDD0663  [3]
 LDCM0347  AC81 HCT 116 C200(1.92)  LDD0664  [3]
 LDCM0348  AC82 HCT 116 C200(0.62)  LDD0665  [3]
 LDCM0248  AKOS034007472 PaTu 8988t C200(1.04)  LDD1127  [3]
 LDCM0356  AKOS034007680 PaTu 8988t C200(1.47)  LDD1235  [3]
 LDCM0275  AKOS034007705 PaTu 8988t C200(1.37)  LDD1154  [3]
 LDCM0367  CL1 HCT 116 C200(1.35)  LDD0684  [3]
 LDCM0368  CL10 HCT 116 C200(1.00)  LDD0685  [3]
 LDCM0369  CL100 PaTu 8988t C200(0.32)  LDD1248  [3]
 LDCM0370  CL101 PaTu 8988t C200(1.27)  LDD1249  [3]
 LDCM0371  CL102 PaTu 8988t C200(1.99)  LDD1250  [3]
 LDCM0372  CL103 PaTu 8988t C200(0.84)  LDD1251  [3]
 LDCM0373  CL104 PaTu 8988t C200(1.42)  LDD1252  [3]
 LDCM0379  CL11 HCT 116 C200(1.00)  LDD0696  [3]
 LDCM0390  CL12 HCT 116 C200(1.12)  LDD0707  [3]
 LDCM0392  CL121 HCT 116 C200(0.79)  LDD0709  [3]
 LDCM0393  CL122 HCT 116 C200(1.16)  LDD0710  [3]
 LDCM0394  CL123 HCT 116 C200(0.92)  LDD0711  [3]
 LDCM0395  CL124 HCT 116 C200(0.94)  LDD0712  [3]
 LDCM0396  CL125 HCT 116 C200(1.65)  LDD0713  [3]
 LDCM0397  CL126 HCT 116 C200(2.04)  LDD0714  [3]
 LDCM0398  CL127 HCT 116 C200(1.38)  LDD0715  [3]
 LDCM0399  CL128 HCT 116 C200(1.03)  LDD0716  [3]
 LDCM0400  CL13 HCT 116 C200(1.03)  LDD0717  [3]
 LDCM0401  CL14 HCT 116 C200(0.98)  LDD0718  [3]
 LDCM0402  CL15 HCT 116 C200(0.69)  LDD0719  [3]
 LDCM0403  CL16 HCT 116 C200(1.06)  LDD0720  [3]
 LDCM0404  CL17 HCT 116 C200(0.68)  LDD0721  [3]
 LDCM0405  CL18 HCT 116 C200(0.62)  LDD0722  [3]
 LDCM0406  CL19 HCT 116 C200(0.59)  LDD0723  [3]
 LDCM0407  CL2 HCT 116 C200(1.37)  LDD0724  [3]
 LDCM0408  CL20 HCT 116 C200(0.75)  LDD0725  [3]
 LDCM0409  CL21 HCT 116 C200(0.67)  LDD0726  [3]
 LDCM0410  CL22 HCT 116 C200(0.60)  LDD0727  [3]
 LDCM0411  CL23 HCT 116 C200(0.83)  LDD0728  [3]
 LDCM0412  CL24 HCT 116 C200(0.92)  LDD0729  [3]
 LDCM0413  CL25 HCT 116 C200(1.10)  LDD0730  [3]
 LDCM0414  CL26 HCT 116 C200(0.93)  LDD0731  [3]
 LDCM0415  CL27 HCT 116 C200(0.78)  LDD0732  [3]
 LDCM0416  CL28 HCT 116 C200(0.85)  LDD0733  [3]
 LDCM0417  CL29 HCT 116 C200(1.41)  LDD0734  [3]
 LDCM0418  CL3 HCT 116 C200(1.52)  LDD0735  [3]
 LDCM0419  CL30 HCT 116 C200(0.84)  LDD0736  [3]
 LDCM0429  CL4 HCT 116 C200(0.96)  LDD0746  [3]
 LDCM0440  CL5 HCT 116 C200(1.12)  LDD0757  [3]
 LDCM0451  CL6 HCT 116 C200(1.16)  LDD0768  [3]
 LDCM0453  CL61 PaTu 8988t C200(1.01)  LDD1332  [3]
 LDCM0454  CL62 PaTu 8988t C200(1.08)  LDD1333  [3]
 LDCM0455  CL63 PaTu 8988t C200(1.60)  LDD1334  [3]
 LDCM0456  CL64 PaTu 8988t C200(1.69)  LDD1335  [3]
 LDCM0457  CL65 PaTu 8988t C200(1.28)  LDD1336  [3]
 LDCM0458  CL66 PaTu 8988t C200(2.23)  LDD1337  [3]
 LDCM0459  CL67 PaTu 8988t C200(1.10)  LDD1338  [3]
 LDCM0460  CL68 PaTu 8988t C200(1.17)  LDD1339  [3]
 LDCM0461  CL69 PaTu 8988t C200(1.19)  LDD1340  [3]
 LDCM0462  CL7 HCT 116 C200(1.13)  LDD0779  [3]
 LDCM0463  CL70 PaTu 8988t C200(1.80)  LDD1342  [3]
 LDCM0464  CL71 PaTu 8988t C200(0.91)  LDD1343  [3]
 LDCM0465  CL72 PaTu 8988t C200(1.17)  LDD1344  [3]
 LDCM0466  CL73 PaTu 8988t C200(0.82)  LDD1345  [3]
 LDCM0467  CL74 PaTu 8988t C200(1.52)  LDD1346  [3]
 LDCM0473  CL8 HCT 116 C200(1.23)  LDD0790  [3]
 LDCM0484  CL9 HCT 116 C200(1.78)  LDD0801  [3]
 LDCM0486  CL91 PaTu 8988t C200(0.26)  LDD1365  [3]
 LDCM0487  CL92 PaTu 8988t C200(0.35)  LDD1366  [3]
 LDCM0488  CL93 PaTu 8988t C200(0.28)  LDD1367  [3]
 LDCM0489  CL94 PaTu 8988t C200(0.49)  LDD1368  [3]
 LDCM0490  CL95 PaTu 8988t C200(0.20)  LDD1369  [3]
 LDCM0491  CL96 PaTu 8988t C200(0.30)  LDD1370  [3]
 LDCM0492  CL97 PaTu 8988t C200(0.27)  LDD1371  [3]
 LDCM0493  CL98 PaTu 8988t C200(0.24)  LDD1372  [3]
 LDCM0494  CL99 PaTu 8988t C200(0.28)  LDD1373  [3]
 LDCM0468  Fragment33 PaTu 8988t C200(1.29)  LDD1347  [3]
 LDCM0022  KB02 ICC108 C77(1.65)  LDD2381  [6]
 LDCM0023  KB03 ICC108 C77(1.92)  LDD2798  [6]
 LDCM0024  KB05 SNU-245 C77(2.12)  LDD3440  [6]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C200(0.49)  LDD2099  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C200(1.18)  LDD2123  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C200(0.56)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C200(0.42)  LDD2137  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) . O14672

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840