Details of the Target

General Information of Target

Target ID LDTP08910
Target Name Protein mono-ADP-ribosyltransferase PARP16 (PARP16)
Gene Name PARP16
Gene ID 54956
Synonyms
ARTD15; C15orf30; Protein mono-ADP-ribosyltransferase PARP16; EC 2.4.2.-; ADP-ribosyltransferase diphtheria toxin-like 15; Poly [ADP-ribose] polymerase 16; PARP-16
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQPSGWAAAREAAGRDMLAADLRCSLFASALQSYKRDSVLRPFPASYARGDCKDFEALLA
DASKLPNLKELLQSSGDNHKRAWDLVSWILSSKVLTIHSAGKAEFEKIQKLTGAPHTPVP
APDFLFEIEYFDPANAKFYETKGERDLIYAFHGSRLENFHSIIHNGLHCHLNKTSLFGEG
TYLTSDLSLALIYSPHGHGWQHSLLGPILSCVAVCEVIDHPDVKCQTKKKDSKEIDRRRA
RIKHSEGGDIPPKYFVVTNNQLLRVKYLLVYSQKPPKRASSQLSWFSSHWFTVMISLYLL
LLLIVSVINSSAFQHFWNRAKR
Target Bioclass
Enzyme
Family
ARTD/PARP family
Subcellular location
Endoplasmic reticulum membrane
Function
Intracellular mono-ADP-ribosyltransferase that plays a role in different processes, such as protein translation and unfolded protein response (UPR), through the mono-ADP-ribosylation of proteins involved in those processes. Acts as an inhibitor of protein translation by catalyzing mono-ADP-ribosylation of ribosomal subunits, such as RPL14 and RPS6, thereby inhibiting polysome assembly and mRNA loading. Mono-ADP-ribosylation of ribosomal subunits is promoted by NMNAT2. Involved in the unfolded protein response (UPR) by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors. May also mediate mono-ADP-ribosylation of karyopherin KPNB1 a nuclear import factor. May not modify proteins on arginine or cysteine residues compared to other mono-ADP-ribosyltransferases.
Uniprot ID
Q8N5Y8
Ensemble ID
ENST00000261888.10
HGNC ID
HGNC:26040
ChEMBL ID
CHEMBL4105981

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 Substitution: p.L72Q .
G361 Substitution: p.L72Q .
IM95 SNV: p.R10M .
MSTO211H Substitution: p.L72Q .
OVCAR8 Substitution: p.G199V .
T98G Substitution: p.L72Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.42  LDD0402  [1]
DBIA
 Probe Info  		C24(2.14)  LDD3367  [2]
AHL-Pu-1
 Probe Info  		C52(2.36)  LDD0168  [3]
IA-alkyne
 Probe Info  		C24(0.00); C52(0.00)  LDD0165  [4]
IPM
 Probe Info  		N.A.  LDD2156  [5]
AOyne
 Probe Info  		15.00  LDD0443  [6]
NAIA_5
 Probe Info  		C24(0.00); C52(0.00)  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C52(2.36)  LDD0168  [3]
 LDCM0026  4SU-RNA+native RNA HEK-293T C52(4.37)  LDD0169  [3]
 LDCM0214  AC1 HEK-293T C169(1.06)  LDD1507  [8]
 LDCM0215  AC10 HEK-293T C169(1.06)  LDD1508  [8]
 LDCM0259  AC14 HEK-293T C169(0.99)  LDD1512  [8]
 LDCM0276  AC17 HEK-293T C169(1.05)  LDD1515  [8]
 LDCM0277  AC18 HEK-293T C169(0.97)  LDD1516  [8]
 LDCM0279  AC2 HEK-293T C169(1.04)  LDD1518  [8]
 LDCM0281  AC21 HEK-293T C169(0.96)  LDD1520  [8]
 LDCM0282  AC22 HEK-293T C169(1.05)  LDD1521  [8]
 LDCM0285  AC25 HEK-293T C169(1.06)  LDD1524  [8]
 LDCM0286  AC26 HEK-293T C169(0.96)  LDD1525  [8]
 LDCM0289  AC29 HEK-293T C169(1.07)  LDD1528  [8]
 LDCM0291  AC30 HEK-293T C169(1.02)  LDD1530  [8]
 LDCM0294  AC33 HEK-293T C169(1.08)  LDD1533  [8]
 LDCM0295  AC34 HEK-293T C169(1.10)  LDD1534  [8]
 LDCM0298  AC37 HEK-293T C169(1.05)  LDD1537  [8]
 LDCM0299  AC38 HEK-293T C169(0.94)  LDD1538  [8]
 LDCM0303  AC41 HEK-293T C169(1.05)  LDD1542  [8]
 LDCM0304  AC42 HEK-293T C169(1.11)  LDD1543  [8]
 LDCM0307  AC45 HEK-293T C169(1.03)  LDD1546  [8]
 LDCM0308  AC46 HEK-293T C169(1.02)  LDD1547  [8]
 LDCM0311  AC49 HEK-293T C169(1.01)  LDD1550  [8]
 LDCM0312  AC5 HEK-293T C169(0.96)  LDD1551  [8]
 LDCM0313  AC50 HEK-293T C169(0.93)  LDD1552  [8]
 LDCM0316  AC53 HEK-293T C169(1.00)  LDD1555  [8]
 LDCM0317  AC54 HEK-293T C169(1.03)  LDD1556  [8]
 LDCM0320  AC57 HEK-293T C169(1.06)  LDD1559  [8]
 LDCM0321  AC58 HEK-293T C169(0.99)  LDD1560  [8]
 LDCM0323  AC6 HEK-293T C169(0.94)  LDD1562  [8]
 LDCM0325  AC61 HEK-293T C169(1.04)  LDD1564  [8]
 LDCM0326  AC62 HEK-293T C169(0.95)  LDD1565  [8]
 LDCM0248  AKOS034007472 HEK-293T C169(0.95)  LDD1511  [8]
 LDCM0356  AKOS034007680 HEK-293T C169(1.04)  LDD1570  [8]
 LDCM0156  Aniline NCI-H1299 12.74  LDD0403  [1]
 LDCM0368  CL10 HEK-293T C169(1.21)  LDD1572  [8]
 LDCM0369  CL100 HEK-293T C169(1.01)  LDD1573  [8]
 LDCM0372  CL103 HEK-293T C169(1.03)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C169(0.94)  LDD1577  [8]
 LDCM0376  CL107 HEK-293T C169(1.06)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C169(1.02)  LDD1581  [8]
 LDCM0381  CL111 HEK-293T C169(1.08)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C169(1.15)  LDD1586  [8]
 LDCM0385  CL115 HEK-293T C169(1.11)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C169(1.04)  LDD1590  [8]
 LDCM0389  CL119 HEK-293T C169(1.02)  LDD1593  [8]
 LDCM0391  CL120 HEK-293T C169(0.98)  LDD1595  [8]
 LDCM0394  CL123 HEK-293T C169(0.98)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C169(1.00)  LDD1599  [8]
 LDCM0398  CL127 HEK-293T C169(1.01)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C169(0.92)  LDD1603  [8]
 LDCM0402  CL15 HEK-293T C169(1.01)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C169(1.02)  LDD1607  [8]
 LDCM0404  CL17 HEK-293T C169(1.16)  LDD1608  [8]
 LDCM0405  CL18 HEK-293T C169(1.03)  LDD1609  [8]
 LDCM0409  CL21 HEK-293T C169(1.06)  LDD1613  [8]
 LDCM0410  CL22 HEK-293T C169(0.96)  LDD1614  [8]
 LDCM0415  CL27 HEK-293T C169(1.00)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C169(0.96)  LDD1620  [8]
 LDCM0417  CL29 HEK-293T C169(0.99)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C169(1.16)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C169(0.88)  LDD1623  [8]
 LDCM0422  CL33 HEK-293T C169(0.93)  LDD1626  [8]
 LDCM0423  CL34 HEK-293T C169(1.01)  LDD1627  [8]
 LDCM0428  CL39 HEK-293T C169(2.99)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C169(0.93)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C169(1.02)  LDD1634  [8]
 LDCM0431  CL41 HEK-293T C169(1.11)  LDD1635  [8]
 LDCM0432  CL42 HEK-293T C169(0.89)  LDD1636  [8]
 LDCM0435  CL45 HEK-293T C169(1.09)  LDD1639  [8]
 LDCM0436  CL46 HEK-293T C169(0.88)  LDD1640  [8]
 LDCM0440  CL5 HEK-293T C169(0.96)  LDD1644  [8]
 LDCM0443  CL52 HEK-293T C169(0.99)  LDD1646  [8]
 LDCM0444  CL53 HEK-293T C169(1.14)  LDD1647  [8]
 LDCM0445  CL54 HEK-293T C169(1.13)  LDD1648  [8]
 LDCM0448  CL57 HEK-293T C169(1.10)  LDD1651  [8]
 LDCM0449  CL58 HEK-293T C169(1.04)  LDD1652  [8]
 LDCM0451  CL6 HEK-293T C169(1.02)  LDD1654  [8]
 LDCM0455  CL63 HEK-293T C169(1.05)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C169(1.17)  LDD1659  [8]
 LDCM0457  CL65 HEK-293T C169(1.10)  LDD1660  [8]
 LDCM0458  CL66 HEK-293T C169(1.02)  LDD1661  [8]
 LDCM0461  CL69 HEK-293T C169(0.92)  LDD1664  [8]
 LDCM0463  CL70 HEK-293T C169(1.02)  LDD1666  [8]
 LDCM0469  CL76 HEK-293T C169(1.04)  LDD1672  [8]
 LDCM0470  CL77 HEK-293T C169(1.08)  LDD1673  [8]
 LDCM0471  CL78 HEK-293T C169(0.95)  LDD1674  [8]
 LDCM0475  CL81 HEK-293T C169(0.92)  LDD1678  [8]
 LDCM0476  CL82 HEK-293T C169(0.96)  LDD1679  [8]
 LDCM0481  CL87 HEK-293T C169(1.03)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C169(0.92)  LDD1685  [8]
 LDCM0483  CL89 HEK-293T C169(1.04)  LDD1686  [8]
 LDCM0484  CL9 HEK-293T C169(0.99)  LDD1687  [8]
 LDCM0485  CL90 HEK-293T C169(0.91)  LDD1688  [8]
 LDCM0488  CL93 HEK-293T C169(0.94)  LDD1691  [8]
 LDCM0489  CL94 HEK-293T C169(1.03)  LDD1692  [8]
 LDCM0494  CL99 HEK-293T C169(1.24)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C169(1.38)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C169(1.00)  LDD1671  [8]
 LDCM0022  KB02 HEK-293T C169(1.03)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C169(0.94)  LDD1497  [8]
 LDCM0024  KB05 NUGC-3 C24(2.14)  LDD3367  [2]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402