Details of the Target

General Information of Target

Target ID LDTP08825
Target Name Protein mono-ADP-ribosyltransferase PARP8 (PARP8)
Gene Name PARP8
Gene ID 79668
Synonyms
Protein mono-ADP-ribosyltransferase PARP8; EC 2.4.2.-; ADP-ribosyltransferase diphtheria toxin-like 16; ARTD16; Poly [ADP-ribose] polymerase 8; PARP-8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPRSVSYSVHVSEDY
PDNTYVSSSENDEDVLVTTEPIPVIFHRIATELRKTNDINCCLSIKSKLQKENGEESRQN
STVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLSAVREIYGPHAVSLREYGAIDD
VDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGL
GHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKS
KSKLKSEQDGISKTHKLLRRTCSSTVKTDDVCVTKSHRTFGRSLSSDPRAEQAMTAIKSH
KLLNRPCPAAVKSEECLTLKSHRLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEEL
YGLKNHKLLSKSYSSAPKSSKTELFKEPNAEGRRLSLTSGLIGILTPSSSSSSQLAPNGA
KCIPVRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQT
LGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKK
NYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQ
LKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRL
QLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKSSNTSQSQKKGQQSQFL
QSRNLKCIALCEVITSSDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIH
KEILRVIGNQTATG
Target Bioclass
Enzyme
Family
ARTD/PARP family
Function Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.
Uniprot ID
Q8N3A8
Ensemble ID
ENST00000281631.10
HGNC ID
HGNC:26124
ChEMBL ID
CHEMBL3091262

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A172 SNV: p.K446Ter .
DU145 SNV: p.C482Y .
EFO21 SNV: p.K305N DBIA    Probe Info 
HCT15 SNV: p.P758T; p.N833K .
HS936T SNV: p.P477S .
HT115 SNV: p.V202A; p.Q474H .
IGR1 SNV: p.G542E DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER SNV: p.P207H .
JHH7 SNV: p.K427N .
MDAMB231 SNV: p.Q19R .
NCIH1793 SNV: p.D756Y .
OV56 SNV: p.P366S .
PC9 SNV: p.H723R .
RH30 SNV: p.R118I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C367(2.17)  LDD3370  [1]
IA-alkyne
 Probe Info  		C297(9.55)  LDD1705  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C367(0.94)  LDD1509  [4]
 LDCM0259  AC14 HEK-293T C367(1.12)  LDD1512  [4]
 LDCM0278  AC19 HEK-293T C367(1.47)  LDD1517  [4]
 LDCM0281  AC21 HEK-293T C367(0.90)  LDD1520  [4]
 LDCM0282  AC22 HEK-293T C367(0.73)  LDD1521  [4]
 LDCM0284  AC24 HEK-293T C367(0.94)  LDD1523  [4]
 LDCM0287  AC27 HEK-293T C367(0.94)  LDD1526  [4]
 LDCM0289  AC29 HEK-293T C367(0.83)  LDD1528  [4]
 LDCM0290  AC3 HEK-293T C367(0.99)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C367(1.05)  LDD1530  [4]
 LDCM0293  AC32 HEK-293T C367(1.21)  LDD1532  [4]
 LDCM0296  AC35 HEK-293T C367(0.94)  LDD1535  [4]
 LDCM0298  AC37 HEK-293T C367(0.84)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C367(0.98)  LDD1538  [4]
 LDCM0302  AC40 HEK-293T C367(1.33)  LDD1541  [4]
 LDCM0305  AC43 HEK-293T C367(0.91)  LDD1544  [4]
 LDCM0307  AC45 HEK-293T C367(1.04)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C367(0.87)  LDD1547  [4]
 LDCM0310  AC48 HEK-293T C367(1.26)  LDD1549  [4]
 LDCM0312  AC5 HEK-293T C367(0.93)  LDD1551  [4]
 LDCM0314  AC51 HEK-293T C367(1.00)  LDD1553  [4]
 LDCM0316  AC53 HEK-293T C367(0.90)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C367(0.96)  LDD1556  [4]
 LDCM0319  AC56 HEK-293T C367(0.87)  LDD1558  [4]
 LDCM0322  AC59 HEK-293T C367(0.89)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C367(1.17)  LDD1562  [4]
 LDCM0325  AC61 HEK-293T C367(1.22)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C367(1.01)  LDD1565  [4]
 LDCM0328  AC64 HEK-293T C367(0.98)  LDD1567  [4]
 LDCM0345  AC8 HEK-293T C367(1.20)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C367(0.99)  LDD1511  [4]
 LDCM0275  AKOS034007705 HEK-293T C367(0.95)  LDD1514  [4]
 LDCM0368  CL10 HEK-293T C367(1.28)  LDD1572  [4]
 LDCM0371  CL102 HEK-293T C367(0.92)  LDD1575  [4]
 LDCM0375  CL106 HEK-293T C367(0.86)  LDD1579  [4]
 LDCM0380  CL110 HEK-293T C367(0.91)  LDD1584  [4]
 LDCM0384  CL114 HEK-293T C367(0.93)  LDD1588  [4]
 LDCM0388  CL118 HEK-293T C367(0.94)  LDD1592  [4]
 LDCM0390  CL12 HEK-293T C367(1.07)  LDD1594  [4]
 LDCM0393  CL122 HEK-293T C367(0.95)  LDD1597  [4]
 LDCM0397  CL126 HEK-293T C367(0.94)  LDD1601  [4]
 LDCM0401  CL14 HEK-293T C367(0.83)  LDD1605  [4]
 LDCM0406  CL19 HEK-293T C367(0.79)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C367(1.05)  LDD1611  [4]
 LDCM0409  CL21 HEK-293T C367(0.79)  LDD1613  [4]
 LDCM0410  CL22 HEK-293T C367(0.94)  LDD1614  [4]
 LDCM0412  CL24 HEK-293T C367(0.92)  LDD1616  [4]
 LDCM0414  CL26 HEK-293T C367(0.91)  LDD1618  [4]
 LDCM0420  CL31 HEK-293T C367(0.99)  LDD1624  [4]
 LDCM0422  CL33 HEK-293T C367(1.04)  LDD1626  [4]
 LDCM0423  CL34 HEK-293T C367(1.30)  LDD1627  [4]
 LDCM0425  CL36 HEK-293T C367(0.84)  LDD1629  [4]
 LDCM0433  CL43 HEK-293T C367(0.97)  LDD1637  [4]
 LDCM0435  CL45 HEK-293T C367(1.03)  LDD1639  [4]
 LDCM0436  CL46 HEK-293T C367(0.85)  LDD1640  [4]
 LDCM0438  CL48 HEK-293T C367(1.08)  LDD1642  [4]
 LDCM0441  CL50 HEK-293T C367(0.90)  LDD1645  [4]
 LDCM0446  CL55 HEK-293T C367(1.00)  LDD1649  [4]
 LDCM0448  CL57 HEK-293T C367(1.05)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C367(0.85)  LDD1652  [4]
 LDCM0452  CL60 HEK-293T C367(1.15)  LDD1655  [4]
 LDCM0454  CL62 HEK-293T C367(0.94)  LDD1657  [4]
 LDCM0459  CL67 HEK-293T C367(0.85)  LDD1662  [4]
 LDCM0461  CL69 HEK-293T C367(0.86)  LDD1664  [4]
 LDCM0462  CL7 HEK-293T C367(1.13)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C367(0.76)  LDD1666  [4]
 LDCM0465  CL72 HEK-293T C367(1.04)  LDD1668  [4]
 LDCM0467  CL74 HEK-293T C367(0.89)  LDD1670  [4]
 LDCM0472  CL79 HEK-293T C367(0.86)  LDD1675  [4]
 LDCM0475  CL81 HEK-293T C367(0.86)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C367(0.99)  LDD1679  [4]
 LDCM0478  CL84 HEK-293T C367(1.27)  LDD1681  [4]
 LDCM0480  CL86 HEK-293T C367(0.91)  LDD1683  [4]
 LDCM0484  CL9 HEK-293T C367(0.96)  LDD1687  [4]
 LDCM0486  CL91 HEK-293T C367(1.30)  LDD1689  [4]
 LDCM0488  CL93 HEK-293T C367(0.94)  LDD1691  [4]
 LDCM0489  CL94 HEK-293T C367(0.99)  LDD1692  [4]
 LDCM0491  CL96 HEK-293T C367(1.21)  LDD1694  [4]
 LDCM0493  CL98 HEK-293T C367(0.86)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C367(0.95)  LDD1631  [4]
 LDCM0022  KB02 A-375 C367(2.50)  LDD2255  [1]
 LDCM0023  KB03 A-375 C367(2.62)  LDD2672  [1]
 LDCM0024  KB05 OCI-AML-2 C367(2.17)  LDD3370  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402