Details of the Target

General Information of Target

Target ID LDTP06337
Target Name Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform (PPP2R5B)
Gene Name PPP2R5B
Gene ID 5526
Synonyms
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform; PP2A B subunit isoform B'-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELT
PLPLLKDVPASELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGV
LIEPVYPDIIRMISVNIFRTLPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPD
FQPSVAKRYVDQKFVLMLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNHIFL
RFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVRVLIPLHSVKSLSVFHAQLAY
CVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQFVKIQEPLFK
QVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSL
IYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKE
APLQRLTPQVAASGGQS
Target Bioclass
Enzyme
Family
Phosphatase 2A regulatory subunit B56 family
Subcellular location
Cytoplasm
Function
As the regulatory component of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.
Uniprot ID
Q15173
Ensemble ID
ENST00000164133.7
HGNC ID
HGNC:9310

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HEC1 SNV: p.R208W .
HEC1B SNV: p.R208W .
HLF SNV: p.E159Q .
IPC298 SNV: p.S32P .
NCIH1666 SNV: p.Q234H .
TOV21G SNV: p.E211Ter .
U2OS SNV: p.R280C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.75  LDD0403  [1]
DBIA
 Probe Info  		C84(0.96)  LDD1507  [2]
AOyne
 Probe Info  		15.00  LDD0443  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C84(0.96)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C113(0.94)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C84(0.94)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C84(1.05)  LDD1510  [2]
 LDCM0270  AC15 HEK-293T C84(1.26)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C84(1.51)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C113(0.79)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C84(1.01)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C113(0.95)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C84(0.78)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C113(1.03)  LDD1520  [2]
 LDCM0283  AC23 HEK-293T C84(0.79)  LDD1522  [2]
 LDCM0285  AC25 HEK-293T C84(1.24)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C113(0.89)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C84(1.05)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C84(0.96)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C113(0.99)  LDD1528  [2]
 LDCM0290  AC3 HEK-293T C84(1.00)  LDD1529  [2]
 LDCM0292  AC31 HEK-293T C84(0.97)  LDD1531  [2]
 LDCM0294  AC33 HEK-293T C84(1.80)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C113(0.82)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C84(0.89)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C84(1.40)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C113(0.90)  LDD1537  [2]
 LDCM0300  AC39 HEK-293T C84(1.39)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C84(0.90)  LDD1540  [2]
 LDCM0303  AC41 HEK-293T C84(1.21)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C113(0.96)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C84(1.02)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C84(0.86)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C113(0.91)  LDD1546  [2]
 LDCM0309  AC47 HEK-293T C84(0.56)  LDD1548  [2]
 LDCM0311  AC49 HEK-293T C84(1.00)  LDD1550  [2]
 LDCM0312  AC5 HEK-293T C113(1.05)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C113(0.95)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C84(1.03)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C84(0.99)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C113(0.99)  LDD1555  [2]
 LDCM0318  AC55 HEK-293T C84(0.78)  LDD1557  [2]
 LDCM0320  AC57 HEK-293T C84(1.43)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C113(0.85)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C84(1.33)  LDD1561  [2]
 LDCM0324  AC60 HEK-293T C84(0.98)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C113(1.05)  LDD1564  [2]
 LDCM0327  AC63 HEK-293T C84(1.01)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C84(1.17)  LDD1568  [2]
 LDCM0248  AKOS034007472 HEK-293T C113(1.00)  LDD1511  [2]
 LDCM0356  AKOS034007680 HEK-293T C84(1.20)  LDD1570  [2]
 LDCM0156  Aniline NCI-H1299 11.75  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C113(1.28); C84(0.82)  LDD1571  [2]
 LDCM0369  CL100 HEK-293T C84(0.89)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C113(1.19); C84(1.42)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C235(0.94); C84(1.18)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C84(0.81)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C84(1.42)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C113(1.11); C84(1.16)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C235(0.91); C84(1.54)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C84(0.98)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C84(0.62)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C113(1.08); C84(1.08)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C84(1.47)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C235(0.96); C84(1.58)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C84(1.06)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C84(1.72)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C113(1.05); C84(1.17)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C235(1.01); C84(1.21)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C84(1.01)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C84(1.27)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C113(1.17); C84(1.68)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C235(0.95); C84(1.15)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C84(0.93)  LDD1593  [2]
 LDCM0391  CL120 HEK-293T C84(0.99)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C113(1.24); C84(1.57)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C235(0.96); C84(1.04)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C84(1.51)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C84(1.03)  LDD1599  [2]
 LDCM0396  CL125 HEK-293T C113(1.22); C84(1.61)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C235(1.12); C84(1.12)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C84(1.05)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C84(1.06)  LDD1603  [2]
 LDCM0400  CL13 HEK-293T C113(1.01); C84(1.05)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C235(1.03); C84(1.27)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C84(0.97)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C84(1.19)  LDD1607  [2]
 LDCM0404  CL17 HEK-293T C84(0.96)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C113(0.95)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C84(1.15)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C235(1.08); C84(1.20)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C84(1.09)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C113(0.96)  LDD1613  [2]
 LDCM0411  CL23 HEK-293T C84(1.15)  LDD1615  [2]
 LDCM0413  CL25 HEK-293T C113(0.92); C84(1.16)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C235(0.85); C84(1.01)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C84(1.03)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C84(1.04)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C84(1.46)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C84(0.83)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C113(0.97)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C84(1.26)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C84(1.10)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C113(0.90)  LDD1626  [2]
 LDCM0424  CL35 HEK-293T C84(1.35)  LDD1628  [2]
 LDCM0426  CL37 HEK-293T C113(1.06); C84(0.89)  LDD1630  [2]
 LDCM0428  CL39 HEK-293T C84(0.88)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C84(1.50)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C84(1.08)  LDD1634  [2]
 LDCM0431  CL41 HEK-293T C84(1.34)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C113(0.90)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C84(1.13)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C84(1.35)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C113(0.95)  LDD1639  [2]
 LDCM0437  CL47 HEK-293T C84(1.02)  LDD1641  [2]
 LDCM0439  CL49 HEK-293T C113(0.94); C84(0.92)  LDD1643  [2]
 LDCM0440  CL5 HEK-293T C84(1.01)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C235(1.00); C84(1.26)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C84(1.70)  LDD1646  [2]
 LDCM0444  CL53 HEK-293T C84(1.15)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C113(0.86)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C84(1.21)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C84(1.15)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C113(1.04)  LDD1651  [2]
 LDCM0450  CL59 HEK-293T C84(1.13)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C113(1.04)  LDD1654  [2]
 LDCM0453  CL61 HEK-293T C113(1.13); C84(0.85)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C235(1.01); C84(0.95)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C84(0.92)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C84(1.26)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C84(1.26)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C113(0.96)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C84(1.05)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C84(1.11)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C113(0.86)  LDD1664  [2]
 LDCM0462  CL7 HEK-293T C84(0.87)  LDD1665  [2]
 LDCM0464  CL71 HEK-293T C84(1.22)  LDD1667  [2]
 LDCM0466  CL73 HEK-293T C113(1.01); C84(0.85)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C235(1.00); C84(1.25)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C84(1.29)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C84(1.48)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C113(0.84)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C84(0.97)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C84(1.36)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C84(0.99)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C113(0.91)  LDD1678  [2]
 LDCM0477  CL83 HEK-293T C84(1.07)  LDD1680  [2]
 LDCM0479  CL85 HEK-293T C113(1.16); C84(1.38)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C235(0.94); C84(1.09)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C84(0.83)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C84(1.48)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C84(0.83)  LDD1686  [2]
 LDCM0484  CL9 HEK-293T C113(0.99)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C113(0.81)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C84(0.82)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C84(1.61)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C113(0.99)  LDD1691  [2]
 LDCM0490  CL95 HEK-293T C84(1.19)  LDD1693  [2]
 LDCM0492  CL97 HEK-293T C113(1.02); C84(1.55)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C235(0.87); C84(1.20)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C84(0.76)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C84(0.82)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C84(0.99)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C235(1.10); C84(1.26)  LDD1631  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) Phosphatase 2A regulatory subunit A family P30153
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) PPP phosphatase family P67775
Serine/threonine-protein kinase Chk2 (CHEK2) CAMK Ser/Thr protein kinase family O96017
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Radiation-inducible immediate-early gene IEX-1 (IER3) IER3 family P46695

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.