Details of the Target

General Information of Target

Target ID LDTP06285
Target Name Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1)
Gene Name ACAP1
Gene ID 9744
Synonyms
CENTB1; KIAA0050; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; Cnt-b1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFV
VGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGF
REARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVI
EDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQ
RHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKK
YKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIA
SAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQC
CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVI
INQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQP
PVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNW
VNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFL
KRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFS
LMASDDPEKLSRRSHDLHTL
Target Bioclass
Other
Subcellular location
Recycling endosome membrane
Function
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Uniprot ID
Q15027
Ensemble ID
ENST00000158762.8
HGNC ID
HGNC:16467

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C320(2.37); C338(2.78)  LDD3334  [1]
DA-P3
 Probe Info  		4.98  LDD0181  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C64(0.00); C328(0.00); C320(0.00); C10(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		C64(0.00); C328(0.00); C320(0.00); C10(0.00)  LDD0036  [3]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [4]
Lodoacetamide azide
 Probe Info  		C64(0.00); C328(0.00); C320(0.00); C10(0.00)  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0031  Epigallocatechin gallate HEK-293T 4.86  LDD0183  [2]
 LDCM0625  F8 Ramos C64(0.67); C79(1.68); C338(1.15)  LDD2187  [5]
 LDCM0572  Fragment10 Ramos C64(1.99); C79(0.97); C338(0.81)  LDD2189  [5]
 LDCM0573  Fragment11 Ramos C64(1.76); C79(0.43); C338(1.27)  LDD2190  [5]
 LDCM0574  Fragment12 Ramos C64(0.68); C79(0.97); C338(0.77)  LDD2191  [5]
 LDCM0575  Fragment13 Ramos C64(0.58); C79(0.76); C338(0.78)  LDD2192  [5]
 LDCM0576  Fragment14 Ramos C64(0.79); C79(2.71)  LDD2193  [5]
 LDCM0579  Fragment20 Ramos C64(0.69); C79(0.85); C338(1.00)  LDD2194  [5]
 LDCM0580  Fragment21 Ramos C64(1.02); C79(0.98); C338(0.95)  LDD2195  [5]
 LDCM0582  Fragment23 Ramos C64(1.05); C79(1.91); C338(0.81)  LDD2196  [5]
 LDCM0578  Fragment27 Ramos C64(0.81); C79(0.91); C338(0.79)  LDD2197  [5]
 LDCM0586  Fragment28 Ramos C64(1.06); C79(0.59); C338(0.83)  LDD2198  [5]
 LDCM0588  Fragment30 Ramos C64(0.53); C79(1.05); C338(0.92)  LDD2199  [5]
 LDCM0589  Fragment31 Ramos C64(0.96); C79(1.19); C338(0.91)  LDD2200  [5]
 LDCM0590  Fragment32 Ramos C64(0.77); C79(0.93); C338(1.11)  LDD2201  [5]
 LDCM0468  Fragment33 Ramos C64(0.73); C79(1.13); C338(1.04)  LDD2202  [5]
 LDCM0596  Fragment38 Ramos C64(0.56); C79(1.10); C338(1.23)  LDD2203  [5]
 LDCM0566  Fragment4 Ramos C64(0.75); C79(0.91); C338(0.65)  LDD2184  [5]
 LDCM0610  Fragment52 Ramos C64(0.38); C79(1.19); C338(0.85)  LDD2204  [5]
 LDCM0614  Fragment56 Ramos C64(0.53); C79(0.86)  LDD2205  [5]
 LDCM0569  Fragment7 Ramos C64(0.68); C79(1.04); C338(1.04)  LDD2186  [5]
 LDCM0571  Fragment9 Ramos C64(1.82); C79(0.82); C338(1.08)  LDD2188  [5]
 LDCM0022  KB02 Ramos C64(0.66); C79(1.48); C338(1.31)  LDD2182  [5]
 LDCM0023  KB03 Ramos C64(0.48); C79(1.57); C338(1.48)  LDD2183  [5]
 LDCM0024  KB05 MOLM-16 C320(2.37); C338(2.78)  LDD3334  [1]
 LDCM0030  Luteolin HEK-293T 5.86  LDD0182  [2]
 LDCM0029  Quercetin HEK-293T 4.98  LDD0181  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) Peptidase M16 family P22695
Protein kinase C alpha type (PRKCA) AGC Ser/Thr protein kinase family P17252
Cytochrome b-c1 complex subunit 7 (UQCRB) UQCRB/QCR7 family P14927
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Nebulette (NEBL) . O76041

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578