Details of the Target

General Information of Target

Target ID LDTP05878
Target Name Tumor suppressor candidate 3 (TUSC3)
Gene Name TUSC3
Gene ID 7991
Synonyms
N33; Tumor suppressor candidate 3; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3; Oligosaccharyl transferase subunit TUSC3; Magnesium uptake/transporter TUSC3; Protein N33
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKKKENLLAEKVEQLMEWS
SRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVCRQANEEYQILANSWRYSS
AFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRPKRADTFDLQRIGFAAEQLAK
WIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLRRNNLEFIYNKTGWAMVSLCIVF
AMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQFVAESHIILVLNAAITMGMVLLNE
AATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFRSKYHGYPYSDLDFE
Target Bioclass
Transporter and channel
Family
OST3/OST6 family
Subcellular location
Endoplasmic reticulum membrane
Function
Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.; Magnesium transporter.
Uniprot ID
Q13454
Ensemble ID
ENST00000382020.8
HGNC ID
HGNC:30242

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AU565 SNV: p.Q94K .
HCT15 SNV: p.G5S .
Ishikawa (Heraklio) 02 ER SNV: p.Y256H .
NCIH146 SNV: p.M57I .
NCIH2172 SNV: p.W231C DBIA    Probe Info 
PF382 SNV: p.T137S DBIA    Probe Info 
RKO Insertion: p.V53SfsTer19 DBIA    Probe Info 
SW1271 SNV: p.R312P .
SW1783 SNV: p.R334H DBIA    Probe Info 
WM793 SNV: p.T201I DBIA    Probe Info 
YSCCC SNV: p.E300K DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K78(7.14)  LDD0277  [1]
OPA-S-S-alkyne
 Probe Info  		K72(2.93)  LDD3494  [2]
BTD
 Probe Info  		C123(2.25)  LDD1700  [3]
DBIA
 Probe Info  		C123(1.26)  LDD0080  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
WYneN
 Probe Info  		N.A.  LDD0021  [6]
IPM
 Probe Info  		N.A.  LDD0005  [6]
Acrolein
 Probe Info  		C123(0.00); H189(0.00)  LDD0217  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C123(0.91); C102(0.75)  LDD2117  [3]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C123(1.41)  LDD2152  [3]
 LDCM0214  AC1 HEK-293T C123(0.94)  LDD1507  [8]
 LDCM0215  AC10 HEK-293T C123(0.93)  LDD1508  [8]
 LDCM0226  AC11 HEK-293T C123(1.01)  LDD1509  [8]
 LDCM0237  AC12 HEK-293T C123(0.78)  LDD1510  [8]
 LDCM0270  AC15 HEK-293T C123(1.02)  LDD1513  [8]
 LDCM0276  AC17 HEK-293T C123(0.92)  LDD1515  [8]
 LDCM0277  AC18 HEK-293T C123(0.83)  LDD1516  [8]
 LDCM0278  AC19 HEK-293T C123(0.85)  LDD1517  [8]
 LDCM0279  AC2 HEK-293T C123(0.91)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C123(0.93)  LDD1519  [8]
 LDCM0283  AC23 HEK-293T C123(1.04)  LDD1522  [8]
 LDCM0285  AC25 HEK-293T C123(0.94)  LDD1524  [8]
 LDCM0286  AC26 HEK-293T C123(0.91)  LDD1525  [8]
 LDCM0287  AC27 HEK-293T C123(1.03)  LDD1526  [8]
 LDCM0288  AC28 HEK-293T C123(0.93)  LDD1527  [8]
 LDCM0290  AC3 HEK-293T C123(1.02)  LDD1529  [8]
 LDCM0292  AC31 HEK-293T C123(0.98)  LDD1531  [8]
 LDCM0294  AC33 HEK-293T C123(0.92)  LDD1533  [8]
 LDCM0295  AC34 HEK-293T C123(0.89)  LDD1534  [8]
 LDCM0296  AC35 HEK-293T C123(0.94)  LDD1535  [8]
 LDCM0297  AC36 HEK-293T C123(0.84)  LDD1536  [8]
 LDCM0300  AC39 HEK-293T C123(1.01)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C123(0.86)  LDD1540  [8]
 LDCM0303  AC41 HEK-293T C123(0.98)  LDD1542  [8]
 LDCM0304  AC42 HEK-293T C123(0.89)  LDD1543  [8]
 LDCM0305  AC43 HEK-293T C123(0.92)  LDD1544  [8]
 LDCM0306  AC44 HEK-293T C123(0.97)  LDD1545  [8]
 LDCM0309  AC47 HEK-293T C123(1.03)  LDD1548  [8]
 LDCM0311  AC49 HEK-293T C123(1.03)  LDD1550  [8]
 LDCM0313  AC50 HEK-293T C123(0.89)  LDD1552  [8]
 LDCM0314  AC51 HEK-293T C123(0.99)  LDD1553  [8]
 LDCM0315  AC52 HEK-293T C123(0.88)  LDD1554  [8]
 LDCM0318  AC55 HEK-293T C123(0.99)  LDD1557  [8]
 LDCM0320  AC57 HEK-293T C123(0.99)  LDD1559  [8]
 LDCM0321  AC58 HEK-293T C123(0.89)  LDD1560  [8]
 LDCM0322  AC59 HEK-293T C123(0.91)  LDD1561  [8]
 LDCM0324  AC60 HEK-293T C123(0.86)  LDD1563  [8]
 LDCM0327  AC63 HEK-293T C123(1.02)  LDD1566  [8]
 LDCM0334  AC7 HEK-293T C123(1.01)  LDD1568  [8]
 LDCM0520  AKOS000195272 MDA-MB-231 C123(1.02); C102(0.86)  LDD2113  [3]
 LDCM0356  AKOS034007680 HEK-293T C123(1.00)  LDD1570  [8]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [7]
 LDCM0367  CL1 HEK-293T C123(0.88)  LDD1571  [8]
 LDCM0369  CL100 HEK-293T C123(1.02)  LDD1573  [8]
 LDCM0370  CL101 HEK-293T C123(0.93)  LDD1574  [8]
 LDCM0371  CL102 HEK-293T C123(0.92)  LDD1575  [8]
 LDCM0372  CL103 HEK-293T C123(1.01)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C123(0.95)  LDD1577  [8]
 LDCM0374  CL105 HEK-293T C123(0.95)  LDD1578  [8]
 LDCM0375  CL106 HEK-293T C123(0.96)  LDD1579  [8]
 LDCM0376  CL107 HEK-293T C123(0.94)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C123(0.95)  LDD1581  [8]
 LDCM0378  CL109 HEK-293T C123(0.94)  LDD1582  [8]
 LDCM0379  CL11 HEK-293T C123(1.07)  LDD1583  [8]
 LDCM0380  CL110 HEK-293T C123(0.84)  LDD1584  [8]
 LDCM0381  CL111 HEK-293T C123(0.89)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C123(0.99)  LDD1586  [8]
 LDCM0383  CL113 HEK-293T C123(1.02)  LDD1587  [8]
 LDCM0384  CL114 HEK-293T C123(0.87)  LDD1588  [8]
 LDCM0385  CL115 HEK-293T C123(1.00)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C123(0.99)  LDD1590  [8]
 LDCM0387  CL117 HEK-293T C123(0.97)  LDD1591  [8]
 LDCM0388  CL118 HEK-293T C123(0.93)  LDD1592  [8]
 LDCM0389  CL119 HEK-293T C123(0.97)  LDD1593  [8]
 LDCM0391  CL120 HEK-293T C123(0.95)  LDD1595  [8]
 LDCM0392  CL121 HEK-293T C123(0.97)  LDD1596  [8]
 LDCM0393  CL122 HEK-293T C123(0.93)  LDD1597  [8]
 LDCM0394  CL123 HEK-293T C123(0.95)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C123(0.94)  LDD1599  [8]
 LDCM0396  CL125 HEK-293T C123(1.02)  LDD1600  [8]
 LDCM0397  CL126 HEK-293T C123(1.03)  LDD1601  [8]
 LDCM0398  CL127 HEK-293T C123(0.98)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C123(0.99)  LDD1603  [8]
 LDCM0400  CL13 HEK-293T C123(0.90)  LDD1604  [8]
 LDCM0401  CL14 HEK-293T C123(0.93)  LDD1605  [8]
 LDCM0402  CL15 HEK-293T C123(0.91)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C123(0.95)  LDD1607  [8]
 LDCM0404  CL17 HEK-293T C123(0.98)  LDD1608  [8]
 LDCM0405  CL18 HEK-293T C123(0.94)  LDD1609  [8]
 LDCM0406  CL19 HEK-293T C123(0.97)  LDD1610  [8]
 LDCM0407  CL2 HEK-293T C123(0.93)  LDD1611  [8]
 LDCM0408  CL20 HEK-293T C123(0.89)  LDD1612  [8]
 LDCM0411  CL23 HEK-293T C123(1.09)  LDD1615  [8]
 LDCM0413  CL25 HEK-293T C123(0.93)  LDD1617  [8]
 LDCM0414  CL26 HEK-293T C123(1.02)  LDD1618  [8]
 LDCM0415  CL27 HEK-293T C123(1.03)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C123(1.04)  LDD1620  [8]
 LDCM0417  CL29 HEK-293T C123(0.92)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C123(1.04)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C123(0.98)  LDD1623  [8]
 LDCM0420  CL31 HEK-293T C123(1.04)  LDD1624  [8]
 LDCM0421  CL32 HEK-293T C123(0.88)  LDD1625  [8]
 LDCM0424  CL35 HEK-293T C123(1.12)  LDD1628  [8]
 LDCM0426  CL37 HEK-293T C123(0.98)  LDD1630  [8]
 LDCM0428  CL39 HEK-293T C123(0.93)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C123(0.92)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C123(1.03)  LDD1634  [8]
 LDCM0431  CL41 HEK-293T C123(1.09)  LDD1635  [8]
 LDCM0432  CL42 HEK-293T C123(0.92)  LDD1636  [8]
 LDCM0433  CL43 HEK-293T C123(0.99)  LDD1637  [8]
 LDCM0434  CL44 HEK-293T C123(1.01)  LDD1638  [8]
 LDCM0437  CL47 HEK-293T C123(1.12)  LDD1641  [8]
 LDCM0439  CL49 HEK-293T C123(0.95)  LDD1643  [8]
 LDCM0440  CL5 HEK-293T C123(1.07)  LDD1644  [8]
 LDCM0441  CL50 HEK-293T C123(0.91)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C123(0.99)  LDD1646  [8]
 LDCM0444  CL53 HEK-293T C123(0.93)  LDD1647  [8]
 LDCM0445  CL54 HEK-293T C123(0.79)  LDD1648  [8]
 LDCM0446  CL55 HEK-293T C123(0.95)  LDD1649  [8]
 LDCM0447  CL56 HEK-293T C123(0.95)  LDD1650  [8]
 LDCM0450  CL59 HEK-293T C123(1.07)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C123(0.87)  LDD1654  [8]
 LDCM0453  CL61 HEK-293T C123(1.00)  LDD1656  [8]
 LDCM0454  CL62 HEK-293T C123(0.91)  LDD1657  [8]
 LDCM0455  CL63 HEK-293T C123(1.00)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C123(0.93)  LDD1659  [8]
 LDCM0457  CL65 HEK-293T C123(0.91)  LDD1660  [8]
 LDCM0458  CL66 HEK-293T C123(0.92)  LDD1661  [8]
 LDCM0459  CL67 HEK-293T C123(1.05)  LDD1662  [8]
 LDCM0460  CL68 HEK-293T C123(0.91)  LDD1663  [8]
 LDCM0462  CL7 HEK-293T C123(1.02)  LDD1665  [8]
 LDCM0464  CL71 HEK-293T C123(1.08)  LDD1667  [8]
 LDCM0466  CL73 HEK-293T C123(0.91)  LDD1669  [8]
 LDCM0467  CL74 HEK-293T C123(0.93)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C123(0.97)  LDD1672  [8]
 LDCM0470  CL77 HEK-293T C123(0.91)  LDD1673  [8]
 LDCM0471  CL78 HEK-293T C123(0.95)  LDD1674  [8]
 LDCM0472  CL79 HEK-293T C123(1.00)  LDD1675  [8]
 LDCM0473  CL8 HEK-293T C123(0.60)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C123(0.89)  LDD1677  [8]
 LDCM0477  CL83 HEK-293T C123(1.05)  LDD1680  [8]
 LDCM0479  CL85 HEK-293T C123(0.94)  LDD1682  [8]
 LDCM0480  CL86 HEK-293T C123(1.00)  LDD1683  [8]
 LDCM0481  CL87 HEK-293T C123(1.01)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C123(0.98)  LDD1685  [8]
 LDCM0483  CL89 HEK-293T C123(1.05)  LDD1686  [8]
 LDCM0485  CL90 HEK-293T C123(0.78)  LDD1688  [8]
 LDCM0486  CL91 HEK-293T C123(0.98)  LDD1689  [8]
 LDCM0487  CL92 HEK-293T C123(0.78)  LDD1690  [8]
 LDCM0490  CL95 HEK-293T C123(0.87)  LDD1693  [8]
 LDCM0492  CL97 HEK-293T C123(1.01)  LDD1695  [8]
 LDCM0493  CL98 HEK-293T C123(0.90)  LDD1696  [8]
 LDCM0494  CL99 HEK-293T C123(0.96)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C123(1.05)  LDD1698  [8]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C123(0.73); C99(0.69)  LDD1702  [3]
 LDCM0468  Fragment33 HEK-293T C123(0.94)  LDD1671  [8]
 LDCM0427  Fragment51 HEK-293T C123(0.93)  LDD1631  [8]
 LDCM0022  KB02 HCT 116 C123(1.26)  LDD0080  [4]
 LDCM0023  KB03 HCT 116 C123(1.08)  LDD0081  [4]
 LDCM0024  KB05 HCT 116 C123(1.24)  LDD0082  [4]
 LDCM0109  NEM HeLa N.A.  LDD0227  [7]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C123(1.30)  LDD2093  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C123(1.14)  LDD2099  [3]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C123(0.85)  LDD2101  [3]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C123(0.75)  LDD2106  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C123(1.11)  LDD2107  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C123(0.93); C102(0.33)  LDD2109  [3]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C123(0.96)  LDD2115  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C102(0.32)  LDD2123  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C123(0.78)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C123(1.02); C102(0.46)  LDD2127  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C123(1.21)  LDD2129  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C123(0.64); C102(0.48)  LDD2134  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C123(1.38)  LDD2135  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C123(1.24)  LDD2136  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C123(2.25)  LDD1700  [3]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C123(0.70)  LDD2141  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C123(3.14)  LDD2144  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C123(0.98)  LDD2146  [3]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C123(1.15)  LDD2148  [3]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402