Details of the Target

General Information of Target

Target ID LDTP05232
Target Name Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (PPP2R2B)
Gene Name PPP2R2B
Gene ID 5521
Synonyms
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; PP2A subunit B isoform B55-beta; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A subunit B isoform beta
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQ
VHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKV
SERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSIS
VNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVY
SSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRD
YLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNN
FFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAW
HPSENIIAVAATNNLYIFQDKVN
Target Bioclass
Enzyme
Family
Phosphatase 2A regulatory subunit B family
Subcellular location
Cytoplasm
Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.
Uniprot ID
Q00005
Ensemble ID
ENST00000336640.10
HGNC ID
HGNC:9305

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.S285L DBIA    Probe Info 
HT115 SNV: p.S190F DBIA    Probe Info 
IGR1 SNV: p.D184N DBIA    Probe Info 
IGROV1 SNV: p.N197S DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER Deletion: p.G46VfsTer4 DBIA    Probe Info 
MFE296 Deletion: p.Q100SfsTer16 DBIA    Probe Info 
MOLT4 SNV: p.A254P .
NCIH2286 SNV: p.Q101H DBIA    Probe Info 
SH4 SNV: p.V395G DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C394(0.61)  LDD2229  [1]
DBIA
 Probe Info  		C364(1.84)  LDD3311  [2]
HHS-475
 Probe Info  		Y82(3.70)  LDD0264  [3]
CY4
 Probe Info  		Q55(0.00); Q60(0.00)  LDD0247  [4]
WYneN
 Probe Info  		N.A.  LDD0021  [5]
WYneO
 Probe Info  		C330(0.00); C258(0.00)  LDD0022  [5]
TFBX
 Probe Info  		N.A.  LDD0148  [6]
HHS-465
 Probe Info  		N.A.  LDD2240  [7]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-2
 Probe Info  		N.A.  LDD0070  [8]
STS-1
 Probe Info  		N.A.  LDD0068  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y82(3.70)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y82(3.47)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y82(3.75)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y82(4.06)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y82(1.76)  LDD0268  [3]
 LDCM0022  KB02 22RV1 C364(1.49)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C364(1.60)  LDD2660  [2]
 LDCM0024  KB05 G361 C364(1.84)  LDD3311  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) Phosphatase 2A regulatory subunit A family P30153
3-phosphoinositide-dependent protein kinase 1 (PDPK1) AGC Ser/Thr protein kinase family O15530
Ribosomal protein S6 kinase beta-1 (RPS6KB1) AGC Ser/Thr protein kinase family P23443
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067

References

1 A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes. Nat Protoc. 2020 Sep;15(9):2891-2919. doi: 10.1038/s41596-020-0352-2. Epub 2020 Jul 20.
Mass spectrometry data entry: PXD016048
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
8 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
9 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.