Details of the Target

General Information of Target

Target ID LDTP05060
Target Name Actin, gamma-enteric smooth muscle (ACTG2)
Gene Name ACTG2
Gene ID 72
Synonyms
ACTA3; ACTL3; ACTSG; Actin, gamma-enteric smooth muscle; EC 3.6.4.-; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin) [Cleaved into: Actin, gamma-enteric smooth muscle, intermediate form]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ
SKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKM
TQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLD
LAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKS
YELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVL
SGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK
PEYDEAGPSIVHRKCF
Target Bioclass
Enzyme
Family
Actin family
Subcellular location
Cytoplasm, cytoskeleton
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Uniprot ID
P63267
Ensemble ID
ENST00000345517.8
HGNC ID
HGNC:145

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA Deletion: p.R29GfsTer16
SNV: p.R29L		 .
CCK81 Deletion: p.R29GfsTer16 .
IGROV1 Deletion: p.R29GfsTer16 .
KPL1 SNV: p.E242K .
MCF7 SNV: p.E242K .
NALM6 SNV: p.R178H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
HHS-475
 Probe Info  		Y241(0.75); Y54(0.77); Y70(0.92); Y189(1.75)  LDD0264  [2]
HHS-465
 Probe Info  		Y189(10.00); Y241(10.00); Y54(10.00); Y70(9.20)  LDD2237  [3]
ATP probe
 Probe Info  		K62(0.00); K329(0.00); K51(0.00)  LDD0035  [4]
AZ-9
 Probe Info  		D26(0.00); E242(0.00)  LDD0395  [5]
NHS
 Probe Info  		K292(0.00); K51(0.00); K327(0.00); K69(0.00)  LDD0010  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [7]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Diazir
 Probe Info  		E242(0.00); E254(0.00); E84(0.00); Y241(0.00)  LDD0011  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y241(0.75); Y54(0.77); Y70(0.92); Y189(1.75)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y241(0.62); Y54(0.67); Y70(0.87); Y189(1.25)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y54(0.71); Y70(0.85); Y189(1.11); Y241(1.16)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y241(0.73); Y54(0.75); Y70(0.97); Y189(1.59)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y241(0.52); Y54(0.71); Y70(0.72); Y189(1.00)  LDD0268  [2]

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
4 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
5 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255