Details of the Target

General Information of Target

Target ID LDTP04511
Target Name Rho GDP-dissociation inhibitor 2 (ARHGDIB)
Gene Name ARHGDIB
Gene ID 397
Synonyms
GDIA2; GDID4; RAP1GN1; Rho GDP-dissociation inhibitor 2; Rho GDI 2; Ly-GDI; Rho-GDI beta
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTEKAPEPHVEEDDDDELDSKLNYKPPPQKSLKELQEMDKDDESLIKYKKTLLGDGPVVT
DPKAPNVVVTRLTLVCESAPGPITMDLTGDLEALKKETIVLKEGSEYRVKIHFKVNRDIV
SGLKYVQHTYRTGVKVDKATFMVGSYGPRPEEYEFLTPVEEAPKGMLARGTYHNKSFFTD
DDKQDHLSWEWNLSIKKEWTE
Target Bioclass
Other
Family
Rho GDI family
Subcellular location
Cytoplasm, cytosol
Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Regulates reorganization of the actin cytoskeleton mediated by Rho family members.
Uniprot ID
P52566
Ensemble ID
ENST00000228945.9
HGNC ID
HGNC:679

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y130(20.00); Y125(13.83); Y107(12.21); Y172(11.81)  LDD0260  [1]
ONAyne
 Probe Info  		K135(0.85)  LDD0274  [2]
STPyne
 Probe Info  		K124(4.44); K50(10.00)  LDD0277  [2]
DBIA
 Probe Info  		C76(1.65)  LDD3334  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [5]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [6]
Compound 11
 Probe Info  		N.A.  LDD2213  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Photocelecoxib
 Probe Info  		E160(0.00); A162(0.00)  LDD0019  [7]
Photonaproxen
 Probe Info  		V159(0.00); P158(0.00)  LDD0157  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C76(0.49)  LDD2187  [8]
 LDCM0572  Fragment10 Ramos C76(0.45)  LDD2189  [8]
 LDCM0573  Fragment11 Ramos C76(0.09)  LDD2190  [8]
 LDCM0574  Fragment12 Ramos C76(0.49)  LDD2191  [8]
 LDCM0575  Fragment13 Ramos C76(0.74)  LDD2192  [8]
 LDCM0576  Fragment14 Ramos C76(0.70)  LDD2193  [8]
 LDCM0579  Fragment20 Ramos C76(0.40)  LDD2194  [8]
 LDCM0580  Fragment21 Ramos C76(0.78)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C76(0.79)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C76(0.75)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C76(0.39)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C76(0.65)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C76(0.78)  LDD2200  [8]
 LDCM0590  Fragment32 Ramos C76(0.45)  LDD2201  [8]
 LDCM0468  Fragment33 Ramos C76(0.77)  LDD2202  [8]
 LDCM0596  Fragment38 Ramos C76(1.13)  LDD2203  [8]
 LDCM0566  Fragment4 Ramos C76(0.60)  LDD2184  [8]
 LDCM0610  Fragment52 Ramos C76(0.85)  LDD2204  [8]
 LDCM0614  Fragment56 Ramos C76(0.95)  LDD2205  [8]
 LDCM0569  Fragment7 Ramos C76(0.39)  LDD2186  [8]
 LDCM0571  Fragment9 Ramos C76(0.57)  LDD2188  [8]
 LDCM0022  KB02 Ramos C76(0.43)  LDD2182  [8]
 LDCM0023  KB03 Ramos C76(0.54)  LDD2183  [8]
 LDCM0024  KB05 MOLM-16 C76(1.65)  LDD3334  [3]
 LDCM0131  RA190 MM1.R C76(1.11)  LDD0304  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) Peptidase M16 family P31930
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein c-Fos (FOS) BZIP family P01100
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
6 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
7 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
9 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.