Details of the Target

General Information of Target

Target ID LDTP04403
Target Name Ras-related protein Rab-27A (RAB27A)
Gene Name RAB27A
Gene ID 5873
Synonyms
RAB27; Ras-related protein Rab-27A; Rab-27; EC 3.6.5.2; GTP-binding protein Ram
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRASGPDG
ATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMH
AYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDL
IMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC
Target Bioclass
Enzyme
Family
Small GTPase superfamily, Rab family
Subcellular location
Membrane
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Uniprot ID
P51159
Ensemble ID
ENST00000336787.6
HGNC ID
HGNC:9766
ChEMBL ID
CHEMBL4105702

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KYM1 SNV: p.T23N .
TCCSUP SNV: p.Y159C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
IA-alkyne
 Probe Info  		C123(1.11)  LDD0304  [2]
BTD
 Probe Info  		C188(1.07)  LDD2129  [3]
HPAP
 Probe Info  		3.01  LDD0063  [4]
IPM
 Probe Info  		N.A.  LDD0025  [5]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
NAIA_5
 Probe Info  		N.A.  LDD2224  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C188(1.17)  LDD2187  [7]
 LDCM0573  Fragment11 Ramos C188(0.04)  LDD2190  [7]
 LDCM0575  Fragment13 Ramos C188(1.04)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C188(1.78)  LDD2193  [7]
 LDCM0580  Fragment21 Ramos C188(0.95)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C188(1.79)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C188(0.90)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C188(0.80)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C188(2.36)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C188(1.01)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C188(2.65)  LDD2201  [7]
 LDCM0468  Fragment33 Ramos C188(1.50)  LDD2202  [7]
 LDCM0596  Fragment38 Ramos C188(2.18)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C188(2.04)  LDD2184  [7]
 LDCM0610  Fragment52 Ramos C188(3.18)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C188(1.34)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C188(2.49)  LDD2186  [7]
 LDCM0022  KB02 Ramos C188(3.78)  LDD2182  [7]
 LDCM0023  KB03 Ramos C188(1.91)  LDD2183  [7]
 LDCM0024  KB05 T cell C123, C131(5.14)  LDD1709  [8]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C188(1.07)  LDD2129  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C188(0.70)  LDD2140  [3]
 LDCM0014  Panhematin K562 3.01  LDD0063  [4]
 LDCM0131  RA190 MM1.R C123(1.11)  LDD0304  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Synaptotagmin-like protein 4 (SYTL4) . Q96C24
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein unc-13 homolog D (UNC13D) Unc-13 family Q70J99
Melanophilin (MLPH) . Q9BV36
Rab effector MyRIP (MYRIP) . Q8NFW9
Synaptotagmin-like protein 1 (SYTL1) . Q8IYJ3
Synaptotagmin-like protein 3 (SYTL3) . Q4VX76

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
8 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.