Details of the Target

General Information of Target

Target ID LDTP04175
Target Name Transcription factor RFX3 (RFX3)
Gene Name RFX3
Gene ID 5991
Synonyms
Transcription factor RFX3; Regulatory factor X 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYV
EGSDTVYTNGAIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQM
GVTGGQLISSSGGTYLIGNSMENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLL
NSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEHKLDPVNAASFGKLIRSIFMGLRTRR
LGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQQKQRYKPMQKVDGVADGFTG
SGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDIKSLQSLYREH
CEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESSNLSEIESRLPKAKLITLCK
HESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQR
MIQTKVAAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASW
VCQCDDNMVQRLETDFKMTLQQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLK
WSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFGDL
NAVSPGNLDKDEGSEVESEMDEELDDSSEPQAKREKTELSQAFPVGCMQPVLETGVQPSL
LNPIHSEHIVTSTQTIRQCSATGNTYTAV
Target Bioclass
Transcription factor
Family
RFX family
Subcellular location
Nucleus
Function
Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
Uniprot ID
P48380
Ensemble ID
ENST00000302303.5
HGNC ID
HGNC:9984

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL33 Deletion: p.V14GfsTer90 .
CAL78 SNV: p.K557N .
MCC26 SNV: p.R255C .
NUGC3 SNV: p.Q270Ter .
WM2664 Insertion: p.S678KfsTer2 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C228(1.84)  LDD3318  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C739(1.00)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C707(0.92)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C707(0.95)  LDD1513  [2]
 LDCM0280  AC20 HEK-293T C739(0.85)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C707(1.07)  LDD1520  [2]
 LDCM0282  AC22 HEK-293T C707(1.42)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C707(0.93)  LDD1522  [2]
 LDCM0288  AC28 HEK-293T C739(0.83)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C707(1.92)  LDD1528  [2]
 LDCM0291  AC30 HEK-293T C707(1.00)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C707(1.33)  LDD1531  [2]
 LDCM0297  AC36 HEK-293T C739(0.95)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C707(1.13)  LDD1537  [2]
 LDCM0299  AC38 HEK-293T C707(0.91)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C707(1.10)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C739(1.01)  LDD1540  [2]
 LDCM0306  AC44 HEK-293T C739(0.89)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C707(1.19)  LDD1546  [2]
 LDCM0308  AC46 HEK-293T C707(1.11)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C707(1.08)  LDD1548  [2]
 LDCM0312  AC5 HEK-293T C707(1.31)  LDD1551  [2]
 LDCM0315  AC52 HEK-293T C739(0.94)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C707(1.33)  LDD1555  [2]
 LDCM0317  AC54 HEK-293T C707(1.40)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C707(0.96)  LDD1557  [2]
 LDCM0323  AC6 HEK-293T C707(1.03)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C739(1.04)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C707(1.02)  LDD1564  [2]
 LDCM0326  AC62 HEK-293T C707(1.05)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C707(1.05)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C707(0.86)  LDD1568  [2]
 LDCM0248  AKOS034007472 HEK-293T C707(0.88)  LDD1511  [2]
 LDCM0368  CL10 HEK-293T C707(1.24)  LDD1572  [2]
 LDCM0379  CL11 HEK-293T C707(1.50)  LDD1583  [2]
 LDCM0408  CL20 HEK-293T C739(1.11)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C707(1.96)  LDD1613  [2]
 LDCM0410  CL22 HEK-293T C707(1.29)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C707(1.07)  LDD1615  [2]
 LDCM0421  CL32 HEK-293T C739(1.26)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C707(1.04)  LDD1626  [2]
 LDCM0423  CL34 HEK-293T C707(1.10)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C707(1.24)  LDD1628  [2]
 LDCM0434  CL44 HEK-293T C739(1.09)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C707(1.23)  LDD1639  [2]
 LDCM0436  CL46 HEK-293T C707(1.26)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C707(1.13)  LDD1641  [2]
 LDCM0447  CL56 HEK-293T C739(1.33)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C707(1.28)  LDD1651  [2]
 LDCM0449  CL58 HEK-293T C707(1.34)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C707(1.16)  LDD1653  [2]
 LDCM0460  CL68 HEK-293T C739(1.07)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C707(1.24)  LDD1664  [2]
 LDCM0463  CL70 HEK-293T C707(1.20)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C707(1.23)  LDD1667  [2]
 LDCM0473  CL8 HEK-293T C739(0.66)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C739(1.09)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C707(1.07)  LDD1678  [2]
 LDCM0476  CL82 HEK-293T C707(1.11)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C707(1.50)  LDD1680  [2]
 LDCM0484  CL9 HEK-293T C707(1.01)  LDD1687  [2]
 LDCM0487  CL92 HEK-293T C739(1.01)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C707(1.01)  LDD1691  [2]
 LDCM0489  CL94 HEK-293T C707(1.62)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C707(1.25)  LDD1693  [2]
 LDCM0022  KB02 A101D C228(1.15)  LDD2250  [1]
 LDCM0023  KB03 A101D C228(2.15)  LDD2667  [1]
 LDCM0024  KB05 MELHO C228(1.84)  LDD3318  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402