Details of the Target

General Information of Target

Target ID LDTP03937
Target Name Ubiquitin-like modifier-activating enzyme 7 (UBA7)
Gene Name UBA7
Gene ID 7318
Synonyms
UBE1L; UBE2; Ubiquitin-like modifier-activating enzyme 7; Ubiquitin-activating enzyme 7; EC 6.2.1.-; D8; Ubiquitin-activating enzyme E1 homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLT
LHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQ
VVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAA
IQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGD
TTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKF
QHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPM
VAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIA
VFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQ
FLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALD
SFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCT
VRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLR
VRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTN
QDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASA
EFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRA
QSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFA
PAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSP
EKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL
Target Bioclass
Enzyme
Family
Ubiquitin-activating E1 family
Function
E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation. Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus. For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition. ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling.
Uniprot ID
P41226
Ensemble ID
ENST00000333486.4
HGNC ID
HGNC:12471
ChEMBL ID
CHEMBL2321623

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C599(20.00)  LDD1464  [1]
DBIA
 Probe Info  		C599(0.95)  LDD0078  [2]
NHS
 Probe Info  		N.A.  LDD0010  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C599(0.95)  LDD0078  [2]
 LDCM0625  F8 Ramos C599(0.89)  LDD2187  [4]
 LDCM0572  Fragment10 MDA-MB-231 C599(8.02)  LDD1465  [1]
 LDCM0574  Fragment12 Ramos C599(2.44)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C599(1.04)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C599(0.91)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C599(3.36)  LDD2194  [4]
 LDCM0580  Fragment21 MDA-MB-231 C599(0.95)  LDD1473  [1]
 LDCM0582  Fragment23 Ramos C599(1.18)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C599(0.91)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C599(0.39)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C599(1.26)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C599(1.24)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C599(2.42)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C599(1.41)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C599(0.88)  LDD2203  [4]
 LDCM0610  Fragment52 Ramos C599(1.84)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C599(1.29)  LDD2205  [4]
 LDCM0571  Fragment9 Ramos C599(20.00)  LDD1464  [1]
 LDCM0022  KB02 T cell C599(6.96)  LDD1703  [5]
 LDCM0023  KB03 Ramos C599(1.00)  LDD2183  [4]
 LDCM0024  KB05 MELHO C145(1.29)  LDD3318  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TAR DNA-binding protein 43 (TARDBP) . Q13148
Ubiquitin-like protein ISG15 (ISG15) . P05161

References

1 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840