Details of the Target

General Information of Target

Target ID LDTP03893
Target Name Proteasome subunit beta type-10 (PSMB10)
Gene Name PSMB10
Gene ID 5699
Synonyms
LMP10; MECL1; Proteasome subunit beta type-10; EC 3.4.25.1; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRAT
NDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTR
ILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDR
FQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGR
YHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE
Target Type
Patented-recorded
Target Bioclass
Enzyme
Family
Peptidase T1B family
Subcellular location
Cytoplasm
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
TTD ID
T09022
Uniprot ID
P40306
DrugMap ID
TTPNACM
Ensemble ID
ENST00000358514.9
HGNC ID
HGNC:9538
ChEMBL ID
CHEMBL3317334

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K101(2.52); K31(10.00)  LDD0277  [1]
DBIA
 Probe Info  		C17(3.17)  LDD3333  [2]
Cep-alkyne
 Probe Info  		3.27  LDD0284  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		C17(0.00); C70(0.00)  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		C17(0.00); C70(0.00)  LDD0037  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
Compound 11
 Probe Info  		N.A.  LDD2213  [5]
IPM
 Probe Info  		N.A.  LDD0147  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0122  Cepafungin 1 RPMI-8226 3.27  LDD0284  [3]
 LDCM0625  F8 Ramos C17(0.77); C70(1.49)  LDD2187  [7]
 LDCM0572  Fragment10 Ramos C17(1.28); C70(0.64)  LDD2189  [7]
 LDCM0573  Fragment11 Ramos C17(0.42)  LDD2190  [7]
 LDCM0574  Fragment12 Ramos C17(1.54); C70(0.56)  LDD2191  [7]
 LDCM0575  Fragment13 Ramos C17(1.00); C70(0.96)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C17(0.94); C70(0.97)  LDD2193  [7]
 LDCM0579  Fragment20 Ramos C17(2.46); C70(0.47)  LDD2194  [7]
 LDCM0580  Fragment21 Ramos C17(1.31); C70(0.83)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C17(0.93); C70(0.89)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C17(1.06); C70(0.92)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C17(0.95); C70(0.52)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C17(1.26); C70(1.14)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C17(1.00); C70(0.81)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C17(2.18); C70(0.78)  LDD2201  [7]
 LDCM0468  Fragment33 Ramos C17(1.36); C70(0.90)  LDD2202  [7]
 LDCM0596  Fragment38 Ramos C17(1.07); C70(0.70)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C17(1.29); C70(0.82)  LDD2184  [7]
 LDCM0610  Fragment52 Ramos C17(1.20); C70(1.26)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C17(2.49); C70(1.16)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C17(1.28); C70(0.71)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C17(2.31); C70(0.67)  LDD2188  [7]
 LDCM0022  KB02 T cell-activated C17(5.54)  LDD1704  [8]
 LDCM0023  KB03 Ramos C17(1.37); C70(0.79)  LDD2183  [7]
 LDCM0024  KB05 MOLM-13 C17(3.17)  LDD3333  [2]
 LDCM0131  RA190 MM1.R C17(2.29)  LDD0304  [9]
 LDCM0170  Structure8 Ramos 5.01  LDD0433  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome subunit beta type-3 (PSMB3) Peptidase T1B family P49720
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cone-rod homeobox protein (CRX) Paired homeobox family O43186
Homeobox protein MOX-2 (MEOX2) . P50222
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-D1-binding protein 1 (CCNDBP1) CCNDBP1 family O95273
Progranulin (GRN) Granulin family P28799
RING finger protein 11 (RNF11) . Q9Y3C5
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Carfilzomib Small molecular drug DB08889
Patented
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Peptide Analog 21 Small molecular drug D02PJI
Peptide Analog 23 Small molecular drug D0T1YB
Peptide Analog 36 Small molecular drug D0DY3A
Peptide Analog 37 Small molecular drug D00GGB
Peptide Analog 38 Small molecular drug D01GON

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Concise Chemoenzymatic Total Synthesis and Identification of Cellular Targets of Cepafungin I. Cell Chem Biol. 2020 Oct 15;27(10):1318-1326.e18. doi: 10.1016/j.chembiol.2020.07.012. Epub 2020 Aug 6.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
8 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
9 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
10 2-Sulfonylpyridines as Tunable, Cysteine-Reactive Electrophiles. J Am Chem Soc. 2020 May 13;142(19):8972-8979. doi: 10.1021/jacs.0c02721. Epub 2020 Apr 29.