Details of the Target

General Information of Target

Target ID LDTP01464
Target Name D-glucuronyl C5-epimerase (GLCE)
Gene Name GLCE
Gene ID 26035
Synonyms
KIAA0836; D-glucuronyl C5-epimerase; EC 5.1.3.17; Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQFPRRSSSGFRVDGFEKRAAASE
SNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGR
REGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGY
NVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAE
DRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKF
LTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRK
GVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKG
GWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFL
SEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYER
GMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKE
FVKRWKSYLKGSRAKHN
Target Bioclass
Enzyme
Family
D-glucuronyl C5-epimerase family
Subcellular location
Golgi apparatus membrane
Function
Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.
Uniprot ID
O94923
Ensemble ID
ENST00000261858.7
HGNC ID
HGNC:17855

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C256(3.02)  LDD3427  [1]
BTD
 Probe Info  		C3(1.05)  LDD2092  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
TFBX
 Probe Info  		N.A.  LDD0027  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C3(1.55)  LDD2142  [2]
 LDCM0259  AC14 HEK-293T C109(1.03); C256(0.88)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C109(1.10)  LDD1513  [5]
 LDCM0281  AC21 HEK-293T C109(1.01)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C109(1.03); C256(0.97)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C109(0.93)  LDD1522  [5]
 LDCM0289  AC29 HEK-293T C109(1.01)  LDD1528  [5]
 LDCM0291  AC30 HEK-293T C109(1.02); C256(1.04)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C109(0.99)  LDD1531  [5]
 LDCM0298  AC37 HEK-293T C109(1.09)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C109(0.95); C256(1.03)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C109(1.06)  LDD1539  [5]
 LDCM0307  AC45 HEK-293T C109(1.00)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C109(1.01); C256(0.99)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C109(1.02)  LDD1548  [5]
 LDCM0312  AC5 HEK-293T C109(1.13)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C109(0.93)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C109(1.02); C256(1.05)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C109(1.12)  LDD1557  [5]
 LDCM0323  AC6 HEK-293T C109(0.99); C256(1.07)  LDD1562  [5]
 LDCM0325  AC61 HEK-293T C109(1.09)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C109(0.99); C256(1.05)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C109(1.02)  LDD1566  [5]
 LDCM0334  AC7 HEK-293T C109(1.12)  LDD1568  [5]
 LDCM0248  AKOS034007472 HEK-293T C109(1.03)  LDD1511  [5]
 LDCM0368  CL10 HEK-293T C109(0.96); C256(1.12)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C109(1.18)  LDD1573  [5]
 LDCM0372  CL103 HEK-293T C109(1.18)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C109(1.10)  LDD1577  [5]
 LDCM0376  CL107 HEK-293T C109(0.98)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C109(1.19)  LDD1581  [5]
 LDCM0379  CL11 HEK-293T C109(1.08)  LDD1583  [5]
 LDCM0381  CL111 HEK-293T C109(1.01)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C109(1.01)  LDD1586  [5]
 LDCM0385  CL115 HEK-293T C109(1.11)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C109(0.98)  LDD1590  [5]
 LDCM0389  CL119 HEK-293T C109(1.17)  LDD1593  [5]
 LDCM0391  CL120 HEK-293T C109(1.04)  LDD1595  [5]
 LDCM0394  CL123 HEK-293T C109(1.05)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C109(1.07)  LDD1599  [5]
 LDCM0398  CL127 HEK-293T C109(1.06)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C109(1.01)  LDD1603  [5]
 LDCM0402  CL15 HEK-293T C109(1.03)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C109(1.20)  LDD1607  [5]
 LDCM0409  CL21 HEK-293T C109(1.28)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C109(1.01); C256(0.95)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C109(1.11)  LDD1615  [5]
 LDCM0415  CL27 HEK-293T C109(1.09)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C109(1.08)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C109(1.24)  LDD1622  [5]
 LDCM0422  CL33 HEK-293T C109(1.14)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C109(0.99); C256(0.95)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C109(0.89)  LDD1628  [5]
 LDCM0428  CL39 HEK-293T C109(1.12)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C109(1.14)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C109(1.18)  LDD1634  [5]
 LDCM0435  CL45 HEK-293T C109(1.03)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C109(1.04); C256(0.86)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C109(0.81)  LDD1641  [5]
 LDCM0443  CL52 HEK-293T C109(1.08)  LDD1646  [5]
 LDCM0448  CL57 HEK-293T C109(1.06)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C109(1.05); C256(1.00)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C109(0.88)  LDD1653  [5]
 LDCM0455  CL63 HEK-293T C109(1.04)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C109(1.01)  LDD1659  [5]
 LDCM0461  CL69 HEK-293T C109(0.94)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C109(1.05); C256(0.81)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C109(1.04)  LDD1667  [5]
 LDCM0469  CL76 HEK-293T C109(1.09)  LDD1672  [5]
 LDCM0475  CL81 HEK-293T C109(1.07)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C109(0.99); C256(0.93)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C109(0.93)  LDD1680  [5]
 LDCM0481  CL87 HEK-293T C109(1.09)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C109(1.12)  LDD1685  [5]
 LDCM0484  CL9 HEK-293T C109(1.15)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C109(1.19)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C109(1.01); C256(1.01)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C109(0.87)  LDD1693  [5]
 LDCM0494  CL99 HEK-293T C109(1.06)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C109(1.13)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C109(1.11)  LDD1671  [5]
 LDCM0022  KB02 BRX394 C256(3.02)  LDD2274  [1]
 LDCM0023  KB03 BRX29 C256(4.57)  LDD2687  [1]
 LDCM0024  KB05 SK-MEL-28 C256(3.02)  LDD3427  [1]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C3(1.05)  LDD2092  [2]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C3(1.24)  LDD2094  [2]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C3(4.88)  LDD2098  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C3(1.37)  LDD2105  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402