Details of the Target

General Information of Target

Target ID LDTP01341
Target Name Cytosolic 10-formyltetrahydrofolate dehydrogenase (ALDH1L1)
Gene Name ALDH1L1
Gene ID 10840
Synonyms
FTHFD; Cytosolic 10-formyltetrahydrofolate dehydrogenase; 10-FTHFDH; FDH; EC 1.5.1.6; Aldehyde dehydrogenase family 1 member L1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWR
AKGQALPDVVAKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAIN
WTLIHGDKKGGFSIFWADDGLDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAV
RLIAEGKAPRLPQPEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQ
KLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILFGNDDKMLLVKNIQLEDGKMI
LASNFFKGAASSVLELTEAELVTAEAVRSVWQRILPKVLEVEDSTDFFKSGAASVDVVRL
VEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGDDEEGECSIDYVEMAVNKRTVRM
PHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKI
SARDRGRLMYRLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKI
QGSTIPINQARPNRNLTLTRKEPVGVCGIIIPWNYPLMMLSWKTAACLAAGNTVVIKPAQ
VTPLTALKFAELTLKAGIPKGVVNVLPGSGSLVGQRLSDHPDVRKIGFTGSTEVGKHIMK
SCAISNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVFFNKGENCIAAGRLFVEDSIH
DEFVRRVVEEVRKMKVGNPLDRDTDHGPQNHHAHLVKLMEYCQHGVKEGATLVCGGNQVP
RPGFFFEPTVFTDVEDHMFIAKEESFGPVMIISRFADGDLDAVLSRANATEFGLASGVFT
RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTF
EY
Target Bioclass
Enzyme
Family
GART family; Aldehyde dehydrogenase family, ALDH1L subfamily
Subcellular location
Cytoplasm, cytosol
Function
Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde.
Uniprot ID
O75891
Ensemble ID
ENST00000273450.7
HGNC ID
HGNC:3978

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.D377V .
CAL78 SNV: p.N861S .
LS180 SNV: p.A116V DBIA    Probe Info 
MCC13 SNV: p.P780S .
MEWO SNV: p.E653K DBIA    Probe Info 
NCIH1650 SNV: p.D346N .
OVK18 SNV: p.A137V; p.P679S; p.G856D .
RKO Deletion: p.E429GfsTer60
SNV: p.E429Q		 .
RL952 SNV: p.R894W .
SG231 SNV: p.R328L .
SNU5 SNV: p.G747R .
SW480 SNV: p.A753S .
TC71 SNV: p.S133F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C707(2.78); C662(3.21)  LDD1699  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
DBIA
 Probe Info  		C248(1.92); C96(1.33)  LDD3319  [3]
WYneC
 Probe Info  		N.A.  LDD0014  [4]
WYneN
 Probe Info  		N.A.  LDD0021  [4]
ENE
 Probe Info  		N.A.  LDD0006  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C662(1.22)  LDD2142  [1]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C662(0.91)  LDD2112  [1]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C662(0.83)  LDD2130  [1]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C662(0.59)  LDD2131  [1]
 LDCM0545  Acetamide MDA-MB-231 C662(0.96); C707(0.65)  LDD2138  [1]
 LDCM0520  AKOS000195272 MDA-MB-231 C662(0.86)  LDD2113  [1]
 LDCM0498  BS-3668 MDA-MB-231 C662(1.03); C707(0.90)  LDD2091  [1]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C662(1.17)  LDD1702  [1]
 LDCM0022  KB02 A101D C248(1.23)  LDD2250  [3]
 LDCM0023  KB03 MDA-MB-231 C662(6.32); C707(1.28)  LDD1701  [1]
 LDCM0024  KB05 MEWO C248(1.92); C96(1.33)  LDD3319  [3]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C662(1.36)  LDD2102  [1]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C707(0.83)  LDD2121  [1]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C662(0.96); C707(0.87)  LDD2089  [1]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C662(1.17)  LDD2092  [1]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C662(2.39)  LDD2094  [1]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C662(1.18)  LDD2098  [1]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C662(0.49)  LDD2100  [1]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C662(0.41)  LDD2101  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C662(0.63)  LDD2104  [1]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C662(1.83)  LDD2105  [1]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C662(0.74)  LDD2106  [1]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C662(0.75)  LDD2108  [1]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C662(0.61)  LDD2114  [1]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C662(0.82)  LDD2124  [1]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C662(1.05)  LDD2126  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C707(0.59)  LDD2141  [1]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C662(0.62); C707(0.97)  LDD2145  [1]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C662(0.72)  LDD2150  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid protein-binding protein 2 (APPBP2) . Q92624

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Tetrahydrofolic Acid Small molecular drug DB00116

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764